Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice

Lee, Se-Eun; Lim, Chi-Yeon; Lim, Seong Jun; Lee, Byoungho; Cho, Su-In

doi:10.1080/13880209.2019.1666883

Cited by 14 publications

(9 citation statements)

References 35 publications

(45 reference statements)

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“…These results, as well as the elevated body weight and organ index, and the changes of the blood lipid levels, generally matched with the results reported for HFD-induced hyperlipidemic mice ( Park et al, 2006 ; Gallou-Kabani et al, 2007 ; Lee et al, 2015 ; Yang et al, 2016 ; Zhong et al, 2018 ; Lee et al, 2019 ), showing the model was successfully developed. All the data for Figs.…”

Section: Resultssupporting

confidence: 87%

Differential proteomic analysis of mouse cerebrums with high-fat diet (HFD)-induced hyperlipidemia

Chen

Wen

Wang

et al. 2022

PeerJ

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Hyperlipidemia is a chronic disease characterized by elevated blood cholesterol and triglycerides and there is accumulated evidence that the disease might affect brain functions. Here we report on a proteomic analysis of the brain proteins in hyperlipidemic mice. Hyperlipidemia was successfully induced in mice by a 20 week high-fat diet (HFD) feeding (model group). A control group with a normal diet and a treatment group with HFD-fed mice treated with a lipid-lowering drug simvastatin (SIM) were established accordingly. The proteins were extracted from the left and right cerebrum hemispheres of the mice in the three groups and subjected to shotgun proteomic analysis. A total of 4,422 proteins were detected in at least half of the samples, among which 324 proteins showed significant difference (fold change >1.5 or <0.67, p < 0.05) in at least one of the four types of comparisons (left cerebrum hemispheres of the model group versus the control group, right cerebrums of model versus control, left cerebrums of SIM versus model, right cerebrums of SIM versus model). Biological process analysis revealed many of these proteins were enriched in the processes correlated with lipid metabolism, neurological disorders, synaptic events and nervous system development. For the first time, it has been reported that some of the proteins have been altered in the brain under the conditions of HFD feeding, obesity or hyperlipidemia. Further, 22 brain processes-related proteins showed different expression in the two cerebrum hemispheres, suggesting changes of the brain proteins caused by hyperlipidemia might also be asymmetric. We hope this work will provide useful information to understand the effects of HFD and hyperlipidemia on brain proteins.

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Section: Resultssupporting

confidence: 87%

Differential proteomic analysis of mouse cerebrums with high-fat diet (HFD)-induced hyperlipidemia

Chen

Wen

Wang

et al. 2022

PeerJ

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“…Metabolomics combined with network pharmacology clarified that Astragalus atropilosulus regulated arachidonic acid metabolism and ether lipid metabolism (Wang et al, 2019). Platycodon grandiflorum and Crataegus pinnatifida ethanol extracts, as well as Ephedra sinica methanol extracts may also improve lipid metabolism (Lee et al, 2017;Kim et al, 2019b;Lee et al, 2019). Remarkably, ephedrine, astragaloside IV, calycosin and icariin, which were detected and identified in present study, may be linked to lipids alterations.…”

Section: Discussionsupporting

confidence: 53%

Huanglong Antitussive Granule Relieves Acute Asthma Through Regulating Pulmonary Lipid Homeostasis

et al. 2021

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Background: Asthma is a respiratory disease with chronic airway inflammatory, and individuals with asthma exacerbations is one of the most frequent causes of hospitalization. Huanglong antitussive granule (HL Granule), a Chinese proprietary herbal medicine, has been proved to be effective in the clinical treatment of pulmonary disease. This study is devoted to the pharmacodynamics of HL Granule in acute asthma and the possible mechanism from the perspective of lipidomics.Methods: Mice were divided into four groups, control group, acute asthma model group, HL Granule treatment and montelukast sodium treatment group. Acute asthma was induced by ovalbumin (OVA). Histopathology, pulmonary function and enzyme linked immunosorbent assay (ELISA) were used to validated model and effect of HL Granule. Lipids were detected by ultra-high-performance liquid chromatography coupled to hybrid Quadrupole-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap MS) and identified by MS-DAIL and built-in Lipidblast database. Differentially expressed lipids recalled in HL Granule treatment group were extracted for heatmap, enrichment analysis and correlation analysis.Results: HL Granule was effective in decreasing airway hyperresponsiveness (AHR), airway inflammatory and the levels of IL-4 and IL-5. A total of 304 and 167 lipids were identified in positive and negative ion mode, respectively. Among these, 104 and 73 lipids were reserved in HL Granule group (FDR < 0.05), including acylcarnitine (ACar), fatty acid (FA), lysophosphatidylcholine (LPC), phosphatidylcholine (PC), lysophosphatidylethanolamine (LPE), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylserine (PS), diglyceride (DG), triglyceride (TG), sphingomyelin (SM) and ceramide (Cer). Furthermore, 118 and 273 correlations among 47 and 96 lipids in the positive and negative were observed, with ether-linked phosphatidylethanolamine (PEe) and phosphatidylcholine (PCe) (FDR < 0.001, Spearman correlation coefficient r2 > 0.75).Conclusion: HL Granule might improve pulmonary lipid homeostasis and could be used as an alternative or supplementary therapy in clinical for the treatment of asthma.

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“…Xin et al pipetted methanol containing 0.1% formic acid onto the tip surface for DI-MS (direct ionization-mass spectrometry)-based metabolomics analysis of three species of medicinal Ephedrae Herba [ 23 ]. Lee et al tested the aqueous and methanol extracts of EH, and found methanol extract shown to be effective and safe to hyperlipidaemic mice [ 24 ]; Lv et al extracted Ephedra sinica with methanol/water (80:20, v/v) for 30 min at room temperature for component analysis [ 25 ]. The Chinese Pharmacopoeia also presented a standard method for several alkaloids quantification as follows: Ephedra sinica were extracted by 1.44% aqueous phosphate solution, and alkaloids were quantified by HPLC method with a mixture solution of methanol, aqueous phosphate, and other adding reagents as the mobile phase.…”

Section: Resultsmentioning

confidence: 99%

Screening and evaluation of anti-SARS-CoV-2 components from Ephedra sinica by ACE2/CMC-HPLC-IT-TOF-MS approach

Wang

Liang

et al. 2021

Anal Bioanal Chem

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Graphical abstract Traditional Chinese medicines played an important role in the treatment of COVID-19 in 2020. Ephedra sinica , one of the major constituent herbs of multi-component herbal formula, has been widely used to treat COVID-19 in China. However, its active components are still unclear. The objectives of this study are to screen and evaluate active components from the traditional Chinese medicine Ephedra sinica for the treatment of COVID-19. In our study, we established an ACE2/CMC bioaffinity chromatography model, and then developed an ACE2/CMC-HPLC-IT-TOF-MS system for the active compounds screening and identification from Ephedra sinica extract. We performed molecular docking and surface plasmon resonance (SPR) assays to assess the binding characteristics (binding mode and K D value). We used CCK-8 staining to assess the toxicity of screened compounds, and also used SARS-CoV-2 pseudovirus to observe the viropexis effect of screened compounds in ACE2 h cells. In this current work, one fraction was fished out, separated and identified as ephedrine (EP), pseudoephedrine (PEP), and methylephedrine (MEP). Binding assays showed that the three compounds could bind with ACE2 in a special way to some amino acid residues, similar to the way SARS-CoV-2 bound with ACE2. Additionally, the three compounds, especially EP, can inhibit the entrance of SARS-CoV-2 spike pseudovirus into ACE2 h cells because they can reduce the entrance ratio of pseudovirus in the pseudovirus model. Overall, the ACE2/CMC-HPLC-IT-TOF-MS system was established and verified to be suitable for ACE2-targeted bioactive compound screening. EP, PEP, and MEP with ACE2-binding features were screened out from Ephedra sinica , and acted as blockers inhibiting SARS-CoV-2 spike pseudovirus entering ACE2 h cells. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03233-7.

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Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice

Cited by 14 publications

References 35 publications

Differential proteomic analysis of mouse cerebrums with high-fat diet (HFD)-induced hyperlipidemia

Differential proteomic analysis of mouse cerebrums with high-fat diet (HFD)-induced hyperlipidemia

Huanglong Antitussive Granule Relieves Acute Asthma Through Regulating Pulmonary Lipid Homeostasis

Screening and evaluation of anti-SARS-CoV-2 components from Ephedra sinica by ACE2/CMC-HPLC-IT-TOF-MS approach

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