Effect of enzyme preparation containing xylanase and ß-glucanase on performance of laying hens fed wheat/barley- or maize/soybean meal-based diets

Abstract

1. A commercial enzyme preparation (Quatrazyme HP) containing xylanase and beta-glucanase was examined in two laying hen experiments with wheat/barley- or maize-based diets. The activities of other enzymes were measured also. Starch, cell wall contents and effects of Quatrazyme HP on in vitro viscosity of wheat, barley, maize and soybean meal were determined. 2. In the first experiment, 90 ISA Brown laying hens at 28 weeks of age were given a wheat/barley basal diet with or without 20mg of Quatrazyme HP, which provided 560 and 2,800 IU of xylanase and beta-glucanase/kg diet. In the second experiment, 66 ISA Brown laying hens at 45 weeks of age were given a maize/soybean meal basal diet with or without 20 mg of Quatrazyme HP/kg diet for 9 weeks. Egg production, egg weight, egg mass, feed conversion ratio and change in body weight were recorded as response criteria. 3. There was a significant improvement in feed conversion ratio with enzyme supplementation. Birds given an enzyme-supplemented diet gained 86 g while those fed on the unsupplemented diet lost 103 g of their body weight by the end of the experiment. 4. The enzyme preparation did not affect either egg production, egg weight or egg mass of birds fed on the maize/soybean meal diet. However, a significant improvement in feed conversion ratio was detected. Birds on either the supplemented or unsupplemented diet exhibited an increase in their body weight at the end of the experiment. 5. Addition of xylanase and beta-glucanase decreased in vitro viscosity of wheat, barley, maize and soybean meal. This effect was greater for wheat and barley than for maize and soybean meal. 6. It was concluded that the beneficial effect of using an enzyme preparation containing xylanase and beta-glucanase is not limited to wheat/barley-based diets but also occurs with maize/soybean meal-based diets.

Metabolizable energy values of diets supplemented with xylanase determined with laying hens

“…In an experiment with broilers, Mathlouthi et al (2003) observed better digestibility of nutrients and of apparent metabolizable energy in diets composed of corn, rice meal and barley, supplemented with xylanase + β-glucanase. Cowieson et al (2006), working with reduction in the level of dietary energy and addition of enzymatic complex (xylanase + protease + amylase + phytase) in diets for broilers observed intake values of digestible fractions (dry matter, nitrogen, energy) greater than or similar to those resulting from supply of diets formulated with adequate nutritional levels without inclusion of enzyme.…”

“…From the results of laboratory analysis information of dry matter intake, metabolizability coefficients of dry matter, crude protein, ether extract, gross energy, value of apparent metabolizable energy and apparent metabolizable energy corrected for nitrogen retention of the feed and nitrogen retention per day were obtained. To calculate the apparent metabolizable energy and apparent metabolizable energy corrected for nitrogen retention and metabolizability coefficients, the formulas described by Matterson et al (1965) apud Sakomura & Rostagno (2007) were used. The data were subjected to analysis of variance by the procedure PROC GLM of SAS (Statistical Analysis System, version 9.1), according to statistical model: Yijk = µ + Ei+ Zj+ (E x Z)ij + eijk in which Yijk = observation of the characteristic in birds of the k experimental unit, of the energy level i and of the form of enzymatic supplementation j; µ = constant common to all experimental units; Ei = effect of the i-th level of dietary energy (i = 1.2); Zj = effect of the j-th form of enzymatic supplementation (j=1.2); EZij = effect of the interaction of the i-th energy level and j-th form of enzymatic supplementation; eijk = error associated with the observation Yijk; eijk ~ N (0, σ 2 ).…”

Effects of wheat cultivar, metabolizable energy level, and xylanase supplementation to laying hens diet on performance, egg quality traits, and selected blood parameters

“…The experimental period could be considered among the factors responsible for differences obtained in the present study. Mathlouthi et al (2003) reported no significant improvement in egg production, egg weight or egg mass when a commercial enzyme preparation containing xylanase and β-glucanase were added to a cornsoybean meal diet fed to 45-wk-old laying hens for 9 wk (from 45 to 54 wk of age). They concluded that enzyme supplementation might be beneficial during production peak because laying hens need high levels of nutrients to maintain body growth and high egg production.…”

Expression of Rumen Microbial Fibrolytic Enzyme Genes in Probiotic Lactobacillus reuteri

“…These antinutritive carbohydrates are undesirable, as they reduce digestion and absorption of all nutrients in the diet, especially fat and protein (8). It has been demonstrated that addition of specific enzymes such as xylanase or ␤-glucanase into wheat-or barley-based diets reduces the antinutritional effect of NSP and leads to better performance of nonruminants (23).…”

“…This reduces the rate of passage, decreases diffusion of digestive enzymes, promotes endogenous losses, and stimulates bacterial proliferation (3). Therefore, it is not surprising that addition of specific enzymes such as xylanase or ␤-glucanase into wheat-or barley-based diets for nonruminant animals decreases viscosity and consequently reduces the antinutritional effect of NSP, leading to better production performance (23). However, enzyme supplementation substantially increases the cost of feed and is only used for a short-term solution in enhancing digestion of cereals.…”