Effect of enzymatic and NaOCl treatments on acrylic roughness and on biofilm accumulation

Abstract

As denture cleansers should be able to reduce biofilm accumulation without changing the acrylic resin surface structure, this study evaluated the effect of denture cleansers on surface roughness of acrylic resin and on biofilm accumulation. It was conducted a crossover study of three phases of 4 days each and 13 volunteers wore palatal appliances containing four specimens of acrylic resin of known surface roughness which were extra-orally submitted once a day to three groups of separated treatments: (i) negative control, (ii) enzymatic commercial solution (Ortoform) or (iii) 0.5% sodium hypochlorite (NaOCl). The appliances were immersed eight times a day in a 20% sucrose solution to enhance biofilm formation. On morning of the fifth day of each phase, the amount of biofilm formed on the specimens was estimated by the amount of protein extracted with alkali and the surface roughness of the specimens was again measured. New palatal appliances with new specimens were worn by the volunteers in the following phases, but the treatments were changed. The roughness of the resin increased after the treatments (P < 0.05) but the difference among the cleansers was not statistically significant (P = 0.85). The lowest amount of biofilm formed on acrylic resin specimens was found for the treatment with NaOCl (P < 0.05) but the enzymatic product did not differ from the negative control group (P > 0.05). The data suggest that the roughness of acrylic resin was not changed by the cleansers, but the ability to reduce biofilm accumulation depended on the product used.

Effect of denture cleansers on metal ion release and surface roughness of denture base materials

“…According to Azevedo et al (24), no alterations in the surface roughness of acrylic resin were found after 7 days of immersion in 1% NaOCl. Also, the results of Lima et al (21) showed no alterations after immersion in 0.5% NaOCl. In the present study, significantly higher surface roughness of the resin was obtained in the specimens treated with Cepacol.…”

“…Acrylic resin plasticizers leach out when they are in contact with chlorine-containing solutions (21). The specimens in this study were immersed in distilled water to eliminate residual monomers (14).…”

Effects of various denture cleansers on surface roughness of hard permanent reline resins

“…Peracini et al 18) reported that alkaline peroxide effervescent denture cleansers significantly increased the surface roughness of heat-polymerized acrylic resin and they should be used with caution. In their crossover in vivo study, Lima et al 19) verified the increased roughness of microwave polymerized base acrylic resins after treatment with enzymatic and sodium hypochlorite solutions.…”

Bactericidal efficacy of glycine-type amphoteric surfactant as a denture cleaner and its influence on properties of denture base resins

“…In particular, poor denture hygiene results in the accumulation of debris and bacterial plaque on the surface of prostheses, causing malodor and inflammatory changes to the adjacent mucosa 3) . For elderly patients who are susceptible to infection, a poorly cleaned oral cavity or prosthesis may induce fatal infections such as aspiration pneumonia and endocarditis 4) .…”