Effect of Environmental Stress during Grain Filling on the Soluble Proteome of Wheat (Triticum aestivum) Dough Liquor

Abstract

The influence of genotype and environment on a soluble wheat dough liquor proteome was studied for four cultivars grown under field conditions and under hot/dry and cool/wet regimes by two-dimensional electrophoresis followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry or quadrupole time-of-flight mass spectrometry. Although the four cultivars had similar patterns, differences in the relative abundances of some components were observed. Similarly, some differences were observed between the control samples and the samples grown under cool/wet and hot/dry conditions. These included differences in the abundances of storage proteins belonging to the 7S globulin (vicilin-like) and alpha-globulin families and of protective proteins including members of the serpin, described as allergens, and chitinase families. A number of novel annotations were made as compared to previous work on the dough liquor of cv. Hereward, including two 19 kDa alpha-globulins, precursors of endochitinases A and C, and several polypeptides belonging to the 7S globulin (vicilin-like) family.

New insights into the effects of high temperature, drought and post-anthesis fertilizer on wheat grain development

“…Eight studies examined the effects of temperature (Dupont et al, 2006a,b;Hurkman et al, 2009;Laino et al, 2010;Majoul et al, 2003Majoul et al, , 2004Skylas et al, 2002;Yang et al, 2011), two studies addressed the effects of drought (Hajheidari et al, 2007;Yang et al, 2011), one study looked at the combined effects of temperature and drought (Sancho et al, 2008) and four studies examined the effects of fertilizer Dupont et al, 2006a,b;Grove et al, 2009). A number of different wheat varieties were used in these studies.…”

“…The high temperature treatments adopted by Hurkman et al (2009) were more prolonged and extended from 10 DPA until maturity while the treatments used by Majoul et al (2003Majoul et al ( , 2004 and Dupont et al (2006a,b) were applied from anthesis to maturity. Sancho et al (2008) combined water deficits with elevated temperatures from 15 DPA until maturity. Yang et al (2011) applied a water deficit from anthesis and Hajheidari et al (2007) limited the amount of water in field grown wheat from the booting stage throughout grain development.…”

Proteomics application of crops in the context of climatic changes

“…In wheat, proteomics-based cultivar identification has been developed for flour (Yahata et al, 2005). Variation in protein composition between cultivars was described in regard to the dough-forming properties of bread wheat (Amiour, Merlino, Leroy, & Branlard, 2002;Salt et al, 2006;Sancho et al, 2008). This methodology was also applied for selection of suitable cultivars for pasta making (De Angelis et al, 2008).…”

“…Generation of reactive oxygen species (ROS) is a common feature of many plants exposed to cold temperatures, which interact with several cellular components including proteins, and plants have been shown to exhibit complementary protective responses to cope with the cold stress (Yamaguchi-Shinozaki & Shinozaki, 2006). Proteomics, or the systematic analysis of the proteins expressed by the genome, have successfully been applied to analyze the cold responsive proteins in many plants including the main staple food crop rice (Cui et al, 2005;Hashimoto & Komatsu, 2007;Hashimoto, Toorchi, Matsushita, Iwasaki, & Komatsu, 2009;Imin, Kerim, Rolfe, & Weinman, 2004;Komatsu, Yamada, & Furukawa, 2009;Lee, Ahsan, Lee, Kang, Lee, et al, 2007b;Lee et al, 2009;Sancho et al, 2008;Yan, Zhang, Tang, Su, & Sun, 2006) (Table 1). Comprehensive analyses of rice leaf proteome in response to cold stress revealed that photosynthetic proteins are largely affected under cold stress (Yan et al, 2006), which might a possible reason for decreasing of net photosynthetic rate and ultimately reduced the crop yield.…”

Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains

“…In our trials, we have observed a downaccumulation of full-length globulins and up-accumulation of those forms corresponding to endo-proteolytic events. Globulin expression is well known to be environmentally dependent in some wheat cultivars (Altenbach and Kothari 2004;Sancho et al 2008;Laino et al 2010;Pompa et al 2013). This class of proteins is multifunctional, especially in seeds.…”