Effect of environmental factors on Fusarium population and associated trichothecenes in wheat grain grown in Jiangsu province, China

Dong, Fei; Qiu, Jianbo; Xu, Jianhong; Yu, Miaojie; Wang, Shufang; Sun, Yi; Zhang, Gufeng; Shi, Jianrong

doi:10.1016/j.ijfoodmicro.2016.04.020

Cited by 53 publications

(24 citation statements)

References 28 publications

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“…Types of crops in crop rotation directly or indirectly promote the infection of cereal crops by Fusarium as the Fusarium ‐contaminated debris from the earlier crop can act as a reservoir for subsequent infection. Previous studies associated with FHB in China have shown that the 15ADON chemotype of F. graminearum was dominant in the northeast and Huanghuaihai regions for wheat–maize rotation, the F. asiaticum ‐3ADON was predominant in the Yangtze River region for wheat–rice rotation, and the NIV chemotype of F. asiaticum was the major chemotype in the southwest region for wheat–rice and rice–ratoon rice rotations (Zhang et al , ; Zhang et al , ; Qiu and Shi, ; Dong et al , ; Hao et al , ; Yang et al , ). F. asiaticum with the 3ADON chemotype was the dominant species in wheat, paddy, and weed samples in Jiangsu Province, but the frequencies of NIV producers in weed and paddy samples were approximately three times higher than in wheat in Jiangsu Province (Zhang et al , ; Qiu and Shi, ; Dong et al , ; Yang et al , ).…”

Section: Discussionmentioning

confidence: 94%

“…F. asiaticum with the 3ADON chemotype was the dominant species in wheat, paddy, and weed samples in Jiangsu Province, but the frequencies of NIV producers in weed and paddy samples were approximately three times higher than in wheat in Jiangsu Province (Zhang et al, 2012;Qiu and Shi, 2014;Dong et al, 2016;Yang et al, 2018).…”

Section: Ta B L E 3 Natural Occurrence Ofmentioning

confidence: 89%

“…Fusarium mycotoxins in gramineous weeds rice-ratoon rice rotations (Zhang et al, 2007;Zhang et al, 2012;Qiu and Shi, 2014;Dong et al, 2016;Hao et al, 2017;Yang et al, 2018).…”

Section: Ta B L E 3 Natural Occurrence Ofmentioning

confidence: 99%

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Gramineous weeds near paddy fields are alternative hosts for the Fusarium graminearum species complex that causes fusarium head blight in rice

Dong

Zhang

et al. 2020

Plant Pathology

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The objective of this study was to evaluate the potential role of gramineous weeds present near paddy fields as alternative hosts for the Fusarium graminearum species complex (FGSC) that causes fusarium head blight (FHB) in rice. A total of 142 weed samples were collected from 10 gramineous weed species near paddy fields from August to October 2018 in Jiangsu Province, China. Of the 145 isolates of seven Fusarium species isolated from the weed samples, F. asiaticum was the most abundant (86.9%), followed by F. fujikuroi (5.5%), F. proliferatum (2.8%), F. graminearum (2.1%), F. tricinctum (1.4%), F. acuminatum (0.7%), and F. sporotrichioides (0.7%). Genotype and mycotoxin analyses confirmed that 72.2% of F. asiaticum isolates were producers of deoxynivalenol (DON) with 3‐acetyl deoxynivalenol (3ADON), and the remainder were nivalenol (NIV) producers. Pathogenicity assays showed that both 3ADON and NIV chemotypes of F. asiaticum could cause FHB in rice, but NIV chemotypes were significantly (p < .05) more aggressive than 3ADON chemotypes. Three Fusarium mycotoxins, DON, NIV, and zearalenone, occurred naturally at low concentrations in the weed samples. Taken together, this study provides insight into the mycotoxin production and aggressiveness of F. asiaticum isolates from gramineous weeds in China.

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Section: Discussionmentioning

confidence: 94%

Section: Ta B L E 3 Natural Occurrence Ofmentioning

confidence: 89%

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Gramineous weeds near paddy fields are alternative hosts for the Fusarium graminearum species complex that causes fusarium head blight in rice

Dong

Zhang

et al. 2020

Plant Pathology

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“…The total amount of ergosterol was extracted from the infected spikelets as described previously (Liu et al, 2013). The DON samples were quantified using LC-MS/MS as described previously (Dong et al, 2016). The experiment was repeated three times.…”

Section: Analyses Of Don Production and Tri Gene Expressionmentioning

confidence: 99%

Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum

Chen

Zheng

et al. 2018

Environmental Microbiology

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Peroxisomal proliferation is highly stimulated during the biosynthesis of mycotoxins and plant infection by Fusarium graminearum. Currently, the functions of the peroxisome in these cellular processes are poorly understood. In this study, we applied genetic, cell biological and biochemical analyses to investigate the functions of the peroxisomes. We constructed targeted deletion of docking machinery components, including FgPex13, FgPex14 and the filamentous fungal specific peroxin FgPex33. Our results indicated that peroxisome dysfunction resulted in a shortage of acetyl-CoA, the precursor of trichothecene biosynthesis, and subsequently decreased deoxynivalenol (DON) production. Deletion mutants of ΔFgPex13, ΔFgPex14 or ΔFgPex33 showed an increased accumulation of endogenous reactive oxygen species (ROS) and reduced phosphorylation of MAP (Mitogen-Activated Protein) kinase FgMgv1. In addition, mutants of the docking peroxin exhibited increased sensitivity toward host oxidative bursts and cell wall integrity stress agents and reduced virulence on host plants. More importantly, we found for the first time that FgPex14 is required for pexophagy in F. graminearum. Overall, our study suggests that peroxisomes play critical roles in DON biosynthesis and virulence in F. graminearum.

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“…After 7 days of incubation, the supernatant was collected for DON quantification. DON samples were quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), as described previously (Dong et al, 2016). The experiment was repeated three times.…”

Section: Plant Infection and Don Production Assaysmentioning

confidence: 99%

The mitochondrial membrane protein FgLetm1 regulates mitochondrial integrity, production of endogenous reactive oxygen species and mycotoxin biosynthesis in Fusarium graminearum

Tang

Zhang

et al. 2018

Molecular Plant Pathology

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Deoxynivalenol (DON) is a mycotoxin produced in cereal crops infected with Fusarium graminearum. DON poses a serious threat to human and animal health, and is a critical virulence factor. Various environmental factors, including reactive oxygen species (ROS), have been shown to interfere with DON biosynthesis in this pathogen. The regulatory mechanisms of how ROS trigger DON production have been investigated extensively in F. graminearum. However, the role of the endogenous ROS-generating system in DON biosynthesis is largely unknown. In this study, we genetically analysed the function of leucine zipper-EF-hand-containing transmembrane 1 (LETM1) superfamily proteins and evaluated the role of the mitochondrial-produced ROS in DON biosynthesis. Our results show that there are two Letm1 orthologues, FgLetm1 and FgLetm2, in F. graminearum. FgLetm1 is localized to the mitochondria and is essential for mitochondrial integrity, whereas FgLetm2 plays a minor role in the maintenance of mitochondrial integrity. The ΔFgLetm1 mutant demonstrated a vegetative growth defect, abnormal conidia and increased sensitivity to various stress agents. More importantly, the ΔFgLetm1 mutant showed significantly reduced levels of endogenous ROS, decreased DON biosynthesis and attenuated virulence in planta. To our knowledge, this is the first report showing that mitochondrial integrity and endogenous ROS production by mitochondria are important for DON production and virulence in Fusarium species.

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Effect of environmental factors on Fusarium population and associated trichothecenes in wheat grain grown in Jiangsu province, China

Cited by 53 publications

References 28 publications

Gramineous weeds near paddy fields are alternative hosts for the Fusarium graminearum species complex that causes fusarium head blight in rice

Gramineous weeds near paddy fields are alternative hosts for the Fusarium graminearum species complex that causes fusarium head blight in rice

Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum

The mitochondrial membrane protein FgLetm1 regulates mitochondrial integrity, production of endogenous reactive oxygen species and mycotoxin biosynthesis in Fusarium graminearum

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