Effect of environmental conditions on various enzyme activities and triacylglycerol contents in cultures of the freshwater diatom, Asterionella formosa (Bacillariophyceae)

Mekhalfi, Malika; Amara, Sawsan; Robert, S.; Carrière, Frédéric; Gontero, Brigitte

doi:10.1016/j.biochi.2013.12.004

Cited by 26 publications

(15 citation statements)

References 66 publications

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“…This increase in GAPDH activity correlates with the increased lipid productivity observed in these two species. GAPDH activity and lipid accumulation are also shown to increase in A. formosa cultured in the presence of plant hormones and high bicarbonate concentrations [13]. This is consistent with GAPDH producing trioses-phosphate that are required building blocks for lipid biosynthesis.…”

Section: Discussionsupporting

confidence: 67%

“…In fact, although we observed a high increased growth of A. formosa grown at high CO 2 , the OD 750 reached a plateau of about 0.08, compared to 0.8-1.0 in the other diatoms analyzed. There is no data in the literature about lipid accumulation in A. formosa grown at high CO 2 , however an increase in both growth and lipid content has been reported in this species grown in bicarbonate (5 mM, compared to 0.15 mM in control) [13]. This difference suggests that A. formosa performance highly depends on the nature of the carbon source supplied to the culture medium and the mechanism used by this diatom to uptake carbon from the environment.…”

Section: Discussionmentioning

confidence: 87%

“…Lipid accumulation in some microalgae species can reach up to 73% of their dry weight and show higher productivities compared to crop plants [9,10]. Within microalgae species, diatoms have emerged as a good source of lipids because of their exceptional photosynthetic efficiency and their ability to accumulate high amounts of lipids in many different culture conditions [6,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Jensen

Yangüez

Carrière

et al. 2019

Biology

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Accumulation of reserve compounds (i.e., lipids and chrysolaminarin) in diatoms depends on the environmental conditions, and is often triggered by stress conditions, such as nutrient limitation. Manipulation of CO2 supply can also be used to improve both lipids and carbohydrates accumulation. Given the high diversity among diatoms, we studied the two marine model diatoms—Thalassiosira pseudonana and Phaeodactylum tricornutum, a freshwater diatom, Asterionella formosa, and Navicula pelliculosa—found in fresh- and sea-water environments. We measured the accumulation of reserve compounds and the activity of enzymes involved in carbon metabolism in these diatoms grown at high and atmospheric CO2. We observed that biomass and lipid accumulation in cells grown at high CO2 differ among the diatoms. Lipid accumulation increased only in P. tricornutum and N. pelliculosa grown in seawater in response to elevated CO2. Moreover, accumulation of lipids was also accompanied by an increased activity of the enzymes tested. However, lipid accumulation and enzyme activity decreased in N. pelliculosa cultured in fresh water. Chrysolaminarin accumulation was also affected by CO2 concentration; however, there was no clear relation with lipids accumulation. Our results are relevant to understand better the ecological role of the environment in the diatom adaptation to CO2 and the mechanisms underpinning the production of storage compounds considering diatom diversity.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Jensen

Yangüez

Carrière

et al. 2019

Biology

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“…Gibberellic acid treatment showed stimulatory effect on lipid content when compared to zeatin; however, the lipid content in gibberellic treated Chlorella was lower than those treated with ABA and IAA. Addition of phytohormones like gibberellic acid resulted in a 2-fold increase in biomass and a 3-fold increase in neutral lipid of a freshwater diatom [30]. In the present study, gibberellic acid treatment resulted in 12% increase in the lipid content of Chlorella.…”

Section: Effect Of Plant Hormones On Lipid Content and Yieldsupporting

confidence: 50%

Plant hormone induced enrichment of Chlorella sp. omega-3 fatty acids

Sivaramakrishnan

Incharoensakdi

2020

Biotechnol Biofuels

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Background: Omega-3 fatty acids have various health benefits in combating against neurological problems, cancers, cardiac problems and hypertriglyceridemia. The main dietary omega-3 fatty acids are obtained from marine fish. Due to the pollution of marine environment, recently microalgae are considered as the promising source for the omega-3 fatty acid production. However, the demand and high production cost associated with microalgal biomass make it necessary to implement novel strategies in improving the biomass and omega-3 fatty acids from microalgae. Results: Four plant hormones zeatin, indole acetic acid (IAA), gibberellic acid (GBA) and abscisic acid (ABA) were investigated for their effect on the production of biomass and lipid in isolated Chlorella sp. The cells showed an increase of the biomass and lipid content after treatments with the plant hormones where the highest stimulatory effect was observed in ABA-treated cells. On the other hand, IAA showed the highest stimulatory effect on the omega-3 fatty acids content, eicosapentaenoic acid (EPA) (23.25%) and docosahexaenoic acid (DHA) (26.06%). On the other hand, cells treated with ABA had highest lipid content suitable for the biodiesel applications. The determination of ROS markers, antioxidant enzymes, and fatty acid biosynthesis genes after plant hormones treatment helped elucidate the mechanism underlying the improvement in biomass, lipid content and omega-3 fatty acids. All four plant hormones upregulated the fatty acid biosynthesis genes, whereas IAA particularly increased omega-3-fatty acids as a result of the upregulation of omega-3 fatty acid desaturase. Conclusions: The contents of omega-3 fatty acids, the clinically important compounds, were considerably improved in IAA-treated cells. The highest lipid content obtained from ABA-treated biomass can be used for biodiesel application according to its biodiesel properties. The EPA and DHA enriched ethyl esters are an approved form of omega-3 fatty acids by US Food and Drug Administration (FDA) which can be utilized as the therapeutic treatment for the severe hypertriglyceridemia.

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“…In the present work, we have established a new, laboratory-based, freshwater model, of intermediate complexity between highly complex natural communities and laboratory pure culture models and have used a combination of cellular, molecular and metagenomics approaches to expand on previous studies. We chose the widespread freshwater pennate diatom Asterionella formosa (Lund, 1949;Happey-Wood and Hughes, 1980;Hayakawa et al, 1994;Mekhalfi et al, 2014aMekhalfi et al, , 2014bVillain et al, 2017) and its associated bacteria as a model system and conducted laboratory investigations on the entire A. formosa-bacterial community, that is, without including intermediate steps of isolation and axenic cultivation of the different partners. This allowed us to study both culturable and unculturable bacteria.…”

Section: Introductionmentioning

confidence: 99%

Exploring the microbiome of the “star” freshwater diatom Asterionella formosa in a laboratory context

Kojadinovic-Sirinelli

Villain

Puppo

et al. 2018

Environmental Microbiology

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Most of our knowledge on the mechanisms underlying diatom-bacterial interactions has been acquired through studies involving isolation of culturable partners. Here, we established a laboratory model of intermediate complexity between complex natural communities and laboratory pure culture models. We investigated the whole community formed by the freshwater diatom Asterionella formosa and its associated bacteria in a laboratory context, including both culturable and unculturable bacteria. Combining cellular and molecular approaches, we showed that in laboratory cultures, A. formosa microbiome was dynamic and comprised of numerous bacterial species (mainly Proteobacteria and Bacteroidetes). Using metagenomics, we explored several metabolic potentials present within the bacterial community. Our analyses suggested that bacteria were heterotrophic although a third of them (Alpha- and Beta-proteobacteria) could also be phototrophic. About 60% of the bacteria, phylogenetically diverse, could metabolize glycolate. The capacity to synthesize molecules such as B vitamins appeared unevenly distributed among bacteria. Altogether, our results brought insights into the bacterial diversity found in diatom-bacterial communities and hinted at metabolic interdependencies within the community that could result in diatom-bacterial and bacterial-bacterial interactions. The present work allowed us to explore the functional architecture of the bacterial community associated with A. formosa in culture and is complementary to field studies.

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Effect of environmental conditions on various enzyme activities and triacylglycerol contents in cultures of the freshwater diatom, Asterionella formosa (Bacillariophyceae)

Cited by 26 publications

References 66 publications

Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Plant hormone induced enrichment of Chlorella sp. omega-3 fatty acids

Exploring the microbiome of the “star” freshwater diatom Asterionella formosa in a laboratory context

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