Effect of endoxylanase and α-l-arabinofuranosidase supplementation on the enzymatic hydrolysis of steam exploded wheat straw

Alvira, Pablo; Negro, M.J.; Ballesteros, Mercedes

doi:10.1016/j.biortech.2010.12.112

Cited by 115 publications

(85 citation statements)

References 29 publications

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“…The enzymes used in this experiment have been studied and reported on in other research works [39] [40] [41] [42]. The enzyme activities determined in this experiment are in the same range as those reported previously, as shown in Table 4.…”

Section: Enzyme Activitysupporting

confidence: 60%

Response Surface Optimization of Enzymatic Hydrolysis of Sugar Beet Leaves into Fermentable Sugars for Bioethanol Production

Aramrueang¹,

Zicari²,

Zhang³

2017

ABB

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Sugar beet leaves are the major crop waste from sugar beet production, while the unused leaves contain a high number of sugars and polysaccharides. The effects of different enzyme products (cellulase, Cellic CTec2; xylanase, Cellic HTec2; and pectinase, Pectinex Ultra SPL) were determined during high-solids enzymatic hydrolysis of sugar beet leaves at 10% total solids (TS) content. Response surface methodology was used to study the effects of enzyme loadings during the hydrolysis of sugar beet leaves for producing fermentable sugars. It was found that both cellulases and pectinases are important enzymes for the hydrolysis of sugar beet leaves. Enzyme loading and reaction time were important factors. Based on the amount of sugars released, a maximum sugar conversion of 82% was achieved after 72 h of hydrolysis using 30 filter paper unit (FPU) g −1 glucan for cellulase and 150 polygalacturonase unit (PGU) g −1 polygalacturonic acid for pectinase, or 37 FPU g −1 glucan for cellulase and 100 PGU g −1 polygalacturonic acid for pectinase. The corresponding sugar yield and sugar concentration were 0.35 g•g −1 TS, and 35 g•l −1 , respectively. Sugar conversion ranged from 59% -70%, 68% -80%, and 74% -82% after 24 h, 48 h, and 72 h of hydrolysis depending on the design conditions.

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Section: Enzyme Activitysupporting

confidence: 60%

Response Surface Optimization of Enzymatic Hydrolysis of Sugar Beet Leaves into Fermentable Sugars for Bioethanol Production

Aramrueang¹,

Zicari²,

Zhang³

2017

ABB

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“…Lignocellulosic wastes are released in large amounts by many industries. Plant cell wall is generally composed of cellulose (35% to 50%), hemicellulose (20% to 35%), and lignin (15% to 20%) ( Figure 1) [20]. Cellulose is the dominant part of lignocellulose and consist of a linear chain of D-glucose linked by β (1-4)-glycosidic bonds to each other.…”

Section: Lignocellulosementioning

confidence: 99%

Lignin Degradation by Fungal Pretreatment: A Review

Madadi¹,

Abbas²

2017

J Plant Pathol Microbiol

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Lignin is regarded as the most plentiful aromatic polymer contains both non-phenolic and phenolic structures. It makes the integral part of secondary wall and plays a significant role in water conduction in vascular plants. Many fungi, bacteria and insects have ability to decrease this lignin by producing enzymes. Among these fungi are the major player in degradation of lignin. These fungi produce enzymes such as lignin peroxidases and laccases. The well-known fungi which degrades lignin are white, brown, soft-rot fungi, and deuteromycetes. Currently these fungi are utilized to reduce lignin to produce ecofriendly bioenergy. The primary aim of this paper is to describe the lignin degradation by biological pre-treatment using fungi (white-, brown-soft-rot fungi and molds), as well as biochemical application. Besides, Molecular methods and enzymes regulation engaged in fungal pre-treatment and some of the factors affecting pretreatment will also be briefly discussed.

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“…Conditions of high temperature and pressure, at 320 °C and 25 MPa respectively, conditions are needed to turn this rigid crystalline structure into an amorphous structure in water. These requirements are higher than for starchy raw materials (60)(61)(62)(63)(64)(65)(66)(67)(68)(69)(70) …”

Section: Cellulosementioning

confidence: 99%

“…The use of hemicellulases, such as endoxylanases, xylosidases, exoxylanases and other accessory enzymes, such as esterases and arabinosidases, improves the efficiency of enzymatic hydrolysis of lignocellulosic biomass (corn stover, herbaceous biomass, barley straw), contributes to the reduction of enzyme loading and lowers costs. The interaction of cellulases with hemicellulases and accessory enzymes makes the entire process of enzymatic hydrolysis effective (69). Various strains of yeasts and bacteria are being investigated with the goal of developing, a consolidated process of hydrolysis and co-fermentation of glucose and xylose, without the need for adding exogenous cellulases (31).…”

Section: Enzymes Used In Industrial Applications Are Produced Mainly mentioning

confidence: 99%

Review of Second-Generation Bioethanol Production from Residual Biomass

Robak

Balcerek

2018

Food Technol. Biotechnol.

467

183

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SummaryIn the context of climate change and the depletion of fossil fuels, there is a great need for alternatives to petroleum in the transport sector. This review provides an overview of the production of second-generation bioethanol, which is distinguished from first-generation and subsequent generations of biofuels by its use of lignocellulosic biomass as raw material. The structural components of the lignocellulosic biomass such as cellulose, hemicellulose and lignin, are presented along with technological unit steps including pre-treatment, enzymatic hydrolysis, fermentation distillation and dehydration. The purpose of the pre-treatment step is to increase the surface area of carbohydrate available for enzymatic saccharification, while minimizing inhibitors. Performing the enzymatic hydrolysis releases fermentable sugars, which are converted by microbial catalysts into ethanol. The hydrolysates obtained after pre-treatment and enzymatic hydrolysis contain a wide spectrum of sugars, predominantly glucose and xylose.Genetically engineered microorganisms are therefore needed to carry out co-fermentation. The excess of harmful inhibitors in the hydrolysate, such as weak organic acids, furan derivatives and phenol components, can be removed by detoxification before fermentation. Effective saccharification further requires using co-acting exogenous hemicellulases and cellulolytic www.ftb.com.hrPlease note that this is an unedited version of the manuscript that has been accepted for publication. This version will undergo copyediting and typesetting before its final form for publication. We are providing this version as a service to our readers. The published version will differ from this one as a result of linguistic and technical corrections and layout editing.enzymes. Conventional species of distillers' yeast are unable to ferment pentoses into ethanol, and only a very few natural microorganisms, including yeast species like Candida shehatae, Pichia stipites, Scheffersomyces and Pachysolen tannophilus, metabolize xylose to ethanol.Enzymatic hydrolysis and fermentation can be performed in a number of ways, by separate saccharification and fermentation, simultaneous saccharification and fermentation or consolidated bioprocessing. Pentose-fermenting microorganisms can be obtained through genetic engineering, by introducing xylose-encoding genes into metabolism of a selected microorganism to optimize its use of xylose accumulated in the hydrolysate.

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Effect of endoxylanase and α-l-arabinofuranosidase supplementation on the enzymatic hydrolysis of steam exploded wheat straw

Cited by 115 publications

References 29 publications

Response Surface Optimization of Enzymatic Hydrolysis of Sugar Beet Leaves into Fermentable Sugars for Bioethanol Production

Response Surface Optimization of Enzymatic Hydrolysis of Sugar Beet Leaves into Fermentable Sugars for Bioethanol Production

Lignin Degradation by Fungal Pretreatment: A Review

Review of Second-Generation Bioethanol Production from Residual Biomass

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