Effect of Endoxylanase and Iron Oxide Nanoparticles on Performance and Histopathological Features in Broilers

Rehman, Hamza; Akram, Muzamal; Kiyani, Mubin Mustafa; Yaseen, Talha; Ghani, Aminuddin Ab; Saggu, Javed Iqbal; Shah, Syed Sajid Hussain; Khalid, Zafar M.; Bokhari, Syed Ali Imran

doi:10.1007/s12011-019-01737-z

Cited by 11 publications

(11 citation statements)

References 31 publications

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“…CuO and FeO NWs were used to investigate their effect upon α-amylase activity to find out any denaturation of α-amylase. For this purpose, α-amylase enzyme was incubated with different parts per million of CuO and FeO NWs (10-70 ppm) to analyze any positive or negative effects on partially purified α-amylase activity as described by Rehman et al (2019). Starch degradation was also studied by subjecting starch substrate to different concentrations of CuO and FeO NWs in the absence of α-amylase as reported by Ernest et al (2012).…”

Section: Partial Purification and Characterization Of α-Amylase Enzymementioning

confidence: 99%

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Ahmad

Khan²,

Yasin

et al. 2021

Appl Nanosci

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α-amylase is the key digestive enzyme that has been used widely in food, paper, detergent and textile industries for starch degradation. This study was conducted for the optimization and characterization of α-amylase production from Aspergillus niger SAIB-4. The study further assessed the effect of metal nanowires (NWs) on starch hydrolysis by α-amylase enzyme from A. niger. Copper oxide (CuO) and iron oxide (FeO) NWs were fabricated in anodized aluminum oxide (AAO) templates. Scanning Electron Microscopy (SEM) and Energy Dispersive spectroscopy (EDS) confirmed the diameter and composition of NWs. Different culture conditions were optimized for the production of α-amylase , where optimum production was obtained at incubation time of 60 h, 5% inoculum size, 1% of banana peel, 0.5% ammonium chloride, pH 8 and 30 °C temperature. It was observed that CuO NWs significantly enhanced α-amylase activity at 40 ppm whereas inhibitory affect was observed for FeO NWs at all concentrations. Maximum starch hydrolysis (0.54 µg/ml) was noticed for CuO NWs at 10 ppm concentration while Minimum activity (0.19 µg/ml) was observed at 70 ppm for FeO NWs. Further, molecular docking analysis was performed to endorse the interaction of NWs with α-amylase with improved enzymatic activity for starch hydrolysis. The combination of α-amylase and CuO NWs could be used as better and efficient source for starch hydrolysis with extraordinary industrial applications, particularly in the sugar and detergent industries.

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Section: Partial Purification and Characterization Of α-Amylase Enzymementioning

confidence: 99%

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Ahmad

Khan²,

Yasin

et al. 2021

Appl Nanosci

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“…Although some reports on the cytotoxic effects of Fe 3 O 4 -NPs have been reported, most of them focus on the research in mammals (rats, mice or humans) [13][14][15][16]. There are few reports on the application of Fe 3 O 4 -NPs in animal husbandry [17,18]. There are zoonotic infectious diseases caused by bacteria such as Salmonella enteritidis, which has led to large numbers of deaths in humans and caused economic losses in animal husbandry.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity studies of Fe₃O₄nanoparticles in chicken macrophage cells

Zhang

Shen

et al. 2020

R. Soc. open sci.

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Magnetic Fe 3 O 4 nanoparticles (Fe 3 O 4 -NPs) have been widely investigated for their biomedical applications. The main purpose of this study was to evaluate the cytotoxic effects of different sizes of Fe 3 O 4 -NPs in chicken macrophage cells (HD11). Experimental groups based on three sizes of Fe 3 O 4 -NPs (60, 120 and 250 nm) were created, and the Fe 3 O 4 -NPs were added to the cells at different doses according to the experimental group. The cell activity, oxidative index (malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS)), apoptosis and pro-inflammatory cytokine secretion level were detected to analyse the cytotoxic effects of Fe 3 O 4 -NPs of different sizes in HD11 cells. The results revealed that the cell viability of the 60 nm Fe 3 O 4 -NPs group was lower than those of the 120 and 250 nm groups when the same concentration of Fe 3 O 4 -NPs was added. No significant difference in MDA was observed among the three Fe 3 O 4 -NP groups. The SOD level and ROS production of the 60 nm group were significantly greater than those of the 120 and 250 nm groups. Furthermore, the highest levels of apoptosis and pro-inflammatory cytokine secretion were caused by the 60 nm Fe 3 O 4 -NPs. In conclusion, the smaller Fe 3 O 4 -NPs produced stronger cytotoxicity in chicken macrophage cells, and the cytotoxic effects may be related to the oxidative stress and apoptosis induced by increased ROS production as well as the increased expression of pro-inflammatory cytokines.

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“…SiO 2 , TiO 2 ZnO and Al 2 O 3 , induce adverse effects in human intestinal cells or experimental animals 28 – 37 . In contrast, gastrointestinal exposure to a variety of iron NPs has not been associated with measurable toxicity 5 , 19 , 26 , 38 – 44 . Short-term feeding of iron deficient rats with diets containing iron NPs 5 , 38 or iron NPs stabilized on β-lactoglobulin fibrils 43 did not cause histopathology or oxidative stress.…”

Section: Discussionmentioning

confidence: 97%

Iron from nanostructured ferric phosphate: absorption and biodistribution in mice and bioavailability in iron deficient anemic women

Baumgartner

Winkler

Zandberg

et al. 2022

Sci Rep

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Food fortification with iron nanoparticles (NPs) could help prevent iron deficiency anemia, but the absorption pathway and biodistribution of iron-NPs and their bioavailability in humans is unclear. Dietary non-heme iron is physiologically absorbed via the divalent metal transporter-1 (DMT1) pathway. Using radio- iron isotope labelling in mice with a partial knockdown of intestine-specific DMT1, we assessed oral absorption and tissue biodistribution of nanostructured ferric phosphate (FePO4-NP; specific surface area [SSA] 98 m2g-1) compared to to ferrous sulfate (FeSO4), the reference compound. We show that absorption of iron from FePO4-NP appears to be largely DMT1 dependent and that its biodistribution after absorption is similar to that from FeSO4, without abnormal deposition of iron in the reticuloendothelial system. Furthermore, we demonstrate high bioavailability from iron NPs in iron deficient anemic women in a randomized, cross-over study using stable-isotope labelling: absorption and subsequent erythrocyte iron utilization from two 57Fe-labeled FePO4-NP with SSAs of 98 m2g−1 and 188 m2g−1 was 2.8-fold and 5.4-fold higher than from bulk FePO4 with an SSA of 25 m2g−1 (P < 0.001) when added to a rice and vegetable meal consumed by iron deficient anemic women. The FePO4-NP 188 m2g-1 achieved 72% relative bioavailability compared to FeSO4. These data suggest FePO4-NPs may be useful for nutritional applications.

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Effect of Endoxylanase and Iron Oxide Nanoparticles on Performance and Histopathological Features in Broilers

Cited by 11 publications

References 31 publications

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Cytotoxicity studies of Fe₃O₄nanoparticles in chicken macrophage cells

Iron from nanostructured ferric phosphate: absorption and biodistribution in mice and bioavailability in iron deficient anemic women

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Effect of Endoxylanase and Iron Oxide Nanoparticles on Performance and Histopathological Features in Broilers

Cited by 11 publications

References 31 publications

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Cytotoxicity studies of Fe3O4nanoparticles in chicken macrophage cells

Iron from nanostructured ferric phosphate: absorption and biodistribution in mice and bioavailability in iron deficient anemic women

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Cytotoxicity studies of Fe₃O₄nanoparticles in chicken macrophage cells