Cytotoxicity studies of Fe₃O₄nanoparticles in chicken macrophage cells

Abstract

Magnetic Fe 3 O 4 nanoparticles (Fe 3 O 4 -NPs) have been widely investigated for their biomedical applications. The main purpose of this study was to evaluate the cytotoxic effects of different sizes of Fe 3 O 4 -NPs in chicken macrophage cells (HD11). Experimental groups based on three sizes of Fe 3 O 4 -NPs (60, 120 and 250 nm) were created, and the Fe 3 O 4 -NPs were added to the cells at different doses according to the experimental group. The cell activity, oxidative index (malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS)), apoptosis and pro-inflammatory cytokine secretion level were detected to analyse the cytotoxic effects of Fe 3 O 4 -NPs of different sizes in HD11 cells. The results revealed that the cell viability of the 60 nm Fe 3 O 4 -NPs group was lower than those of the 120 and 250 nm groups when the same concentration of Fe 3 O 4 -NPs was added. No significant difference in MDA was observed among the three Fe 3 O 4 -NP groups. The SOD level and ROS production of the 60 nm group were significantly greater than those of the 120 and 250 nm groups. Furthermore, the highest levels of apoptosis and pro-inflammatory cytokine secretion were caused by the 60 nm Fe 3 O 4 -NPs. In conclusion, the smaller Fe 3 O 4 -NPs produced stronger cytotoxicity in chicken macrophage cells, and the cytotoxic effects may be related to the oxidative stress and apoptosis induced by increased ROS production as well as the increased expression of pro-inflammatory cytokines.

Magnetite Nanoparticles in Magnetic Hyperthermia and Cancer Therapies: Challenges and Perspectives

“…Cytotoxicity of nanomaterials depends on the concentration, proportion, porosity, aggregation tendency, a chemical affinity for biological structures, chemical surface reactivity and even the shape, size of nanoparticles, and environmental conditions [ 183 , 184 ]. So far, many studies have been conducted on the cytotoxicity of magnetite [ 31 , 111 , 138 , 185 , 186 , 187 , 188 ]. First of all, it has been noticed that the previously mentioned particle size influences the cytotoxicity of magnetite.…”

“…First of all, it has been noticed that the previously mentioned particle size influences the cytotoxicity of magnetite. When using chicken macrophages, it was found that smaller magnetite particles (60, 120, and 250 nm were used) were more toxic to these cells [ 188 ]. No significant toxicity of particles with a size of 30 μm was detected on HeLa cells and postnatal human fibroblasts [ 138 ].…”

Cytotoxicity Effect of Iron Oxide (Fe3O4)/Graphene Oxide (GO) Nanosheets in Cultured HBE Cells

“…It is worth noting that cell viability was less than 60% after 48 h, even at low concentrations (20 μg/ml). However, other reports indicated that these nanoparticles had the potential to produce toxic effects in cells, and their toxic effects were related to their size, concentration, time, shape, and the cell type ( Wang et al, 2020 ; Zhang S. et al, 2020 ). Thus, we also verified the cytotoxicity effects of Fe 3 O 4 /GO in BEAS-2B cells, which also belong to human lung epithelial cells.…”

Therapeutic and Toxicological Aspects of Some Metal Nanoparticles on The Central Nervous System: A Review

“…Silver nanoparticles offer remarkable antibacterial properties and have been employed in food services, building materials, the textile sector, medical devices, personal care items, and washing machines. Silver nanoparticles are utilized in laundry detergents, room sprays, deodorants, wall paints, and indoor air purifiers and water detoxifiers [51]. Due to widespread usage and human exposure to silver nanoparticles, it is feasible for Ag nanoparticles to enter the body and build up in many tissues and organs.…”