Effect of Endotoxin on Cells and on Their Response to Infection by Polioviruses

Abstract

The effect of lipopolysaceharide on HeLa-S3,

Effect of Triiodothyronine on Cells and on Their Response to Infection by Polioviruses

“…Cell strains and media. The origin of the strains of HeLa-S3, HeLa-Gey, Chang-liver, and Maben cells was reported previously (Murphy and Wisner, 1962). The growth medium used for the propagation of cells consisted of Eagle medium (80%) and human serum (20%).…”

“…Culture fluids were adjusted with 1.4 % NaHCO3 solution to a pH of 7.4 to 7.6 and maintained at this level by addition of a feeding solution (Murphy and 641 on October 1, 2020 by guest http://jb.asm.org/ Downloaded from MURPHY AND BULLIS Wisner, 1962). The maintenance inedium (Murphy and Wisner, 1962) used to sustain the cells consisted of: Hanks balanced salt solution (BSS), 72.5 ml; 5% lactalbumin hydrolysate, 10 ml; 1 % yeast extract, 10 ml; 4.4% NaHCO3 solution, 2.0 ml; 10% glucose solution, 2.5 ml; 1 ml of a solution containing 0.128 g of sodium pyruvate and 0.226 g of sodium acetate per 100 ml of BSS; and calf serum, 3.0 ml. None of the cell culture additives contained antibiotics.…”

“…Effect of TS on morphology of cells in monolayer culture. Growth medium was decanted from monolayer cultures (Murphy and Wisner, 1962), the cells washed three times with the buffered salt solution (GKN) described by Merchant, Kahn, and Murphy (1960), and graded concentrations of T3 in maintenance medium added. Five replicate culture tubes were used for each concentration of T3: 0.1, 1.0, 10, 20, 30, 40, 50, and 60 /Ag/ml; five control tubes contained maintenance medium free of T3.…”

“…Effect of TS on colonies of cells. The procedure used to plate cells was previously described (Murphy and Wisner, 1962). Cell colonies were washed three times with GKN, and dilutions of T3 in maintenance medium were added.…”

“…After addition of T3, cultures were incubated at 37 C for 8 days. A record was kept of the rate of destruction by T3 of compact and diffuse cell colonies (Murphy and Wisner, 1962).…”