Effect of endothelin‐1 and endothelin receptor blockade on the release of microparticles

Jung, Christian; Lichtenauer, Michael; Wernly, Bernhard; Franz, Marcus; Goebel, Bjoern; Rafnsson, Arnar; Figulla, Hans‐Reiner; Pernow, John

doi:10.1111/eci.12652

Cited by 8 publications

(7 citation statements)

References 30 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because activated endothelial cells increase expression of cell adhesion molecules, the reductions in shear may have increased uptake of endothelial MPs (Jansen et al., 2015). ET‐1 binding contributes to endothelial MP release in vitro (Jung et al., 2016), and has been proposed as a contributor to the sitting‐induced vasculopathy (Padilla & Fadel, 2017). However, we observed no changes in ET‐1 after sitting, with or without calf raises, which conflicts with previous reports showing consistent rises during prolonged sitting (Ballard et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

Sitting decreases endothelial microparticles but not circulating angiogenic cells irrespective of lower leg exercises: a randomized cross‐over trial

Evans

Hanson

Shill

et al. 2020

Experimental Physiology

View full text Add to dashboard Cite

Repeated exposure to prolonged sitting increases the risk for cardiovascular disease. However, the cellular links by which repeated exposure to prolonged sitting lead to increased cardiovascular risk have not been fully elucidated, with markers of vascular damage and repair such as microparticles (MPs) and circulating angiogenic cell (CACs) being promising targets. The objective of the study was to examine the effects of 3 h of sitting with or without intermittent calf raises on annexin V + /CD34 + , annexin V + /CD62E + , and annexin V + /CD31 + /42b − MP populations linked to CAC paracrine activity, endothelial activation and apoptosis, respectively, as well as CD14 + /31 + , CD3 + /31 + , and CD34 + CACs, which are linked to endothelial repair. In a random order, 20 sedentary participants (14 females, 22 ± 3 years) remained seated for 180 min with or without performing 10 calf raises every 10 min. Blood samples were obtained after 20 min of quiet rest in the supine position before and after sitting. Overall, sitting decreased annexin V + /CD34 + MPs (−12 ± 5 events µl −1 , P < 0.01), annexin V + /CD62E + MPs (−17 ± 4 events µl −1 , P < 0.001), and annexin V + /CD31 + /42b − MPs (−22 ± 6 events µl −1 , P < 0.001) regardless of condition. There were no differences in endothelin-1 plasma concentration, CD14 + /31 + , CD34 + or CD3 + /31 + CAC frequencies. Sitting did not alter CAC number, but decreased MPs linked to endothelial activation, apoptosis and CAC paracrine activity in a manner that was independent of muscle contraction. These findings support changes in markers of endothelial activation and apoptosis with sedentary behaviour and provide new insights into altered intercellular communication with physical inactivity such as prolonged sitting.

show abstract

Section: Discussionmentioning

confidence: 99%

Sitting decreases endothelial microparticles but not circulating angiogenic cells irrespective of lower leg exercises: a randomized cross‐over trial

Evans

Hanson

Shill

et al. 2020

Experimental Physiology

View full text Add to dashboard Cite

show abstract

“…However, in patients with diabetes, bosentan did not affect EMP levels. 85 Recently, we have determined that staying at 2978 m above sea level resulted in a significant decrease of EMP levels. Still, the additional intake of the endothelin receptor blocker Macitentan did not influence EMP concentrations nor cardiovascular biomarkers.…”

Section: Effect Of Endothelin-1 and Endothelin Receptor Blockade Onmentioning

confidence: 99%

Research update for articles published in EJCI in 2016

Adlbrecht

Blanco-Verea

Bouzas-Mosquera

et al. 2018

Eur J Clin Investigation

Self Cite

View full text Add to dashboard Cite

“…Microparticles are defined as small phospholipid vesicles without a nucleus that are released from different cells, platelets, erythrocytes, leukocytes and endothelial cells [17–19]. Endothelial cell microparticles (EMP) can be identified by antigens expressed by mature endothelial cells such as CD31 [20].…”

Section: Introductionmentioning

confidence: 99%

Microparticles in patients undergoing transcatheter aortic valve implantation (TAVI)

et al. 2016

Self Cite

View full text Add to dashboard Cite

Degenerative aortic stenosis (AS) is the most frequent form of acquired valvular heart disease. AS is known to entail endothelial dysfunction caused by increased mechanical shear stress leading to elevated circulatory levels of microparticles. Endothelial and platelet microparticles (EMP and PMP) are small vesicles that originate from activated cells and thrombocytes. We sought to evaluate whether transcatheter aortic valve implantation (TAVI) procedure would elicit effects on circulating EMP and PMP. 92 patients undergoing TAVI procedure for severe AS were included in this study. Samples were obtained at each visit before TAVI, 1 week post-procedure and at 1, 3 and after 6 months after TAVI and were evaluated using flow cytometry. A 12 month clinical follow-up was also performed. CD62E+ EMP concentration before TAVI was 21.11 % (±6.6 % SD) and declined to 20.99 % (±6.8 % SD) after 1 week, to 16.63 % (±5.4 % SD, p < 0.0001) after 1 month, to 17.08 % (±4.6 % SD, p < 0.0001) after 3 months and to 15.94 % (±5.4 % SD, p < 0.0001) after 6 months. CD31+/CD42b−, CD31+/Annexin+/− EMP remained unchanged. CD31+/CD41b+ PMP evidenced a slight, but statistically significant increase after TAVI and remained elevated during the entire follow-up. Apart from a procedure-related improvement in echocardiographic parameters, TAVI procedure led also to a decline in CD62E+ EMP. The reduction in pressure gradients with less hemodynamic shear stress seems also to have beneficially affected endothelial homeostasis.Electronic supplementary materialThe online version of this article (doi:10.1007/s00380-016-0885-z) contains supplementary material, which is available to authorized users.

show abstract

Effect of endothelin‐1 and endothelin receptor blockade on the release of microparticles

Cited by 8 publications

References 30 publications

Sitting decreases endothelial microparticles but not circulating angiogenic cells irrespective of lower leg exercises: a randomized cross‐over trial

Sitting decreases endothelial microparticles but not circulating angiogenic cells irrespective of lower leg exercises: a randomized cross‐over trial

Research update for articles published in EJCI in 2016

Microparticles in patients undergoing transcatheter aortic valve implantation (TAVI)

Contact Info

Product

Resources

About