Effect of endophytic fungi on the host plant growth, expression of expansin gene and flavonoid content in Tetrastigma hemsleyanum Diels &amp; Gilg ex Diels

Song, Yuping; Wu, Pan; Li, Yafei; Tong, Xiaxiu; Yong, Zheng; Chen, Zhehao; Wang, Lilin; Tu, Xiang

doi:10.1007/s11104-017-3266-1

Cited by 28 publications

(12 citation statements)

References 34 publications

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“…australis USA OTU54 KT923222 — — — — Clay et al. (2016) Tetrastigma hemsleyanum China TH11 KY607743 — — — — Song et al. (2017) Fuscosclera lignicola Dead wood Spain CBS:142287T NR_164252 — — — — Hernández-Restrepo et al.…”

Section: Methodsmentioning

confidence: 99%

Mollisiaceae: An overlooked lineage of diverse endophytes

Tanney

Seifert

2020

Studies in Mycology

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Mollisia is a taxonomically neglected discomycete genus ( Helotiales, Leotiomycetes ) of commonly encountered saprotrophs on decaying plant tissues throughout temperate regions. The combination of indistinct morphological characters, more than 700 names in the literature, and lack of reference DNA sequences presents a major challenge when working with Mollisia . Unidentified endophytes, including strains that produced antifungal or antiinsectan secondary metabolites, were isolated from conifer needles in New Brunswick and placed with uncertainty in Phialocephala and Mollisia , necessitating a more comprehensive treatment of these genera. In this study, morphology and multigene phylogenetic analyses were used to explore the taxonomy of Mollisiaceae , including Mollisia , Phialocephala , and related genera, using new field collections, herbarium specimens, and accessioned cultures and sequences. The phylogeny of Mollisiaceae was reconstructed and compared using the nuc internal transcribed spacer rDNA (ITS) barcode and partial sequences of the 28S nuc rDNA (LSU) gene, largest subunit of RNA polymerase II ( RPB1 ), DNA topoisomerase I ( TOP1 ), and the hypothetical protein Lipin/Ned1/Smp2 ( LNS2 ). The results show that endophytism is common throughout the Mollisiaceae lineage in a diverse range of hosts but is infrequently attributed to Mollisia because of a paucity of reference sequences. Generic boundaries within Mollisiaceae are poorly resolved and based on phylogenetic evidence the family included species placed in Acephala , Acidomelania , Barrenia , Bispora , Cheirospora , Cystodendron , Fuscosclera , Hysteronaevia, Loramyces, Mollisia, Neopyrenopeziza , Obtectodiscus, Ombrophila, Patellariopsis , Phialocephala , Pulvinata , Tapesia (= Mollisia ), and Trimmatostroma. Taxonomic novelties included the description of five novel Mollisia species and five novel Phialocephala species and the synonymy of Fuscosclera with Phialocephala , Acidomelania with Mollisia , and Loramycetaceae with Mollisiaceae .

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Section: Methodsmentioning

confidence: 99%

Mollisiaceae: An overlooked lineage of diverse endophytes

Tanney

Seifert

2020

Studies in Mycology

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“…The complete genomic sequence of TDG has been reported to enrich TDG plant information ( Li et al., 2016 ). Song et al. (2017) isolate 31 endophytic fungi belonging to 10 genera and add fermented broth of endophyte strains (named TH09, TH12, TH14, TH15, TH17, and TH26 respectively) to MS medium to culture axenic seedlings.…”

Section: Quality Controlmentioning

confidence: 99%

The Phytochemistry, Pharmacology, and Quality Control of Tetrastigma hemsleyanum Diels & Gilg in China: A Review

Zhu

Ying

et al. 2020

Front. Pharmacol.

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Tetrastigma hemsleyanum Diels & Gilg (TDG), the family member of Vitaceae, is a traditional herbal medicine in China. The root of TDG can be immediately used after cleaning the muddy soil, and can be dehydrated for dry use. TDG is able to be collected all year round, which is commonly used in the treatment of hepatitis, infantile high fever, snake bite, etc. Based on phytochemistry, the chemical components of TDG are divided into flavonoids, phenolic acids, terpenes, steroids, polysaccharide, and other compounds, showing many pharmacological effects which include anti-tumor, antioxidation, anti-inflammatory, antipyretic, analgesic, and immunomodulatory activity, as well as other activities. Currently, TDG involves some problems of the reduction of wild resources, the backward processing methods, and storage difficulties as well as the imperfection of detection methods. Therefore, this review summarizes the literature of the past 20 years, and the purpose of this review is to summarize the recent researches on the phytochemistry, pharmacology, quality control, and clinical application of TDG. The above discussions provide new insights for the future research on TDG.

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“…Referring to the method of Du et al (2015) and Song et al (2017) [2,26], the gourd-shaped roots with a diameter of about 1.5 cm were selected, cut and dried at 70°C for 6 h to form powder. Powder (0.1 g) was weighted and put into a 5 mL centrifuge tube and 1.5 mL of 50% ethanol was added.…”

Section: Determination Of Total Flavonoids In Root Of T Hemsleyanummentioning

confidence: 99%

The effect of rhizosphere soil on the flavonoid metabolism in the roots of Tetrastigma hemsleyanum

Tu¹,

Tong²,

Li³

et al. 2019

Preprint

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Background: Tetrastigma hemsleyanum (T. hemsleyanum) is a precious and rare traditional Chinese medicinal plant. Flavonoid is its main medicinal ingredient. Wild T. hemsleyanum (W-TH) growing in Zhejiang Province, China is recognized as a medicinal material of “San Ye Qing” Dao-di herbs. The different origins and thus the contents of medicinal ingredients are the key criteria used to determine whether the medicinal materials are authentic Dao-di herbs. However, it is less known how the eco-environments of its specific producing area, especially microbial community in rhizosphere soil, affect the content of medicinal ingredients in “San Ye Qing”. In the present study, we determined the content of total flavonoids and the enzymatic activity of phenylalanine ammonia-lyase (PAL) in the roots of W-TH and artificially cultivated T. hemsleyanum (C-TH), as well as the nutrient compositions and metagenome in rhizosphere soil. The effects of the rhizosphere soil on the flavonoid metabolism in T. hemsleyanum were evaluated. Results: The contents of total flavonoids and the PAL activity were higher in W-TH root than in C-TH root. The contents of both available phosphorus and available potassium were higher in the rhizosphere soil of W-TH than in that of C-TH, while the contents of nitrogen and organic matter in the rhizosphere root of C-TH were higher than that of W-TH. Compared with the rhizosphere of C-TH, the abundances of genera Enterobacter, Serratia, Raoultella, Kluyvera, Comamonas, Acinetobacte, and Arthrobacter, and the pathways related to nitrogen metabolism, inositol phosphate metabolism, phosphinate metabolism, and phosphotransferase system in the rhizosphere of W-TH were significantly different. There existed differences in phenylpropanoid biosynthesis and phenylalanine metabolism in the rhizospheres of W-TH and C-TH. Conclusions: The contents of nitrogen and available phosphorus in the rhizosphere soil affect diversity abundance, nitrogen metabolism, phosphorus metabolism, and phenylalanine (Phe) anabolism of rhizosphere microorganisms. They may further affect Phe content and PAL activity for the synthesis of flavonoids in the root of T. hemsleyanum.

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Effect of endophytic fungi on the host plant growth, expression of expansin gene and flavonoid content in Tetrastigma hemsleyanum Diels & Gilg ex Diels

Cited by 28 publications

References 34 publications

Mollisiaceae: An overlooked lineage of diverse endophytes

Mollisiaceae: An overlooked lineage of diverse endophytes

The Phytochemistry, Pharmacology, and Quality Control of Tetrastigma hemsleyanum Diels & Gilg in China: A Review

The effect of rhizosphere soil on the flavonoid metabolism in the roots of Tetrastigma hemsleyanum

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