Effect of Encapsulation on Viability of Pediococcus pentosaceus OZF During Its Passage Through the Gastrointestinal Tract Model

Abstract

The main goal of this study was to develop an improved oral delivery system for Pediococcus pentosaceus OZF, a promising probiotic bacterium, and to assess its viability under simulated gastrointestinal (GI) tract model by comparing the efficiency of microbiological and molecular approaches. Encapsulation was carried out using extrusion method and as a result, encapsulation system including 0.75 % lactulose, 1.8 % sodium alginate, 0.1 M CaCl2, and 5 min gelling time was shown to have a significantly protective effect against pH 2.0 acid stress over 3 h. However, completely loss of viability was exhibited by free OZF cells under similar conditions. To provide an additional barrier for capsules, coating process was investigated using different biopolymers, and the survival rates of free and encapsulated OZF cells upon expose to simulated GI conditions were detected by conventional culture techniques and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR) method. No significant differences between the biopolymers were detected, except the chitosan which leads totally 85 % protection and extra 25 % improvement in the survival of OZF cells compared to uncoated capsules. In conclusion, our findings indicated that chitosan-coated capsules provided an important protective effect on the viability of OZF cells against the GI system conditions encountered during the transit of food. In addition, this study was found successful in monitoring the viable OZF cells in capsules using PMA-qPCR method.

Control of Listeria monocytogenes biofilm on industrial surfaces by cell ‐ free extracts of Lactobacillus plantarum

“…After biofilm formation and co‐incubation assays, each of the coupons was removed from the well and washed by sterile PBS three times to remove the medium and nonadhered cells. Following the fixation of the coupons at room temperature in 4% formaldehyde for 3 hr, the washing step was repeated and the coupons were dehydrated in serial dilutions of 30, 50, 60, 70, and 90% ethanol for 5 min each, followed by three rinses for 10 min in 100% ethanol (Kiran et al., 2015). Finally, each of the coupons was air‐dried and immediately sputter coated with gold (WD = 50 mm, 90 s, 30 mA), then examined using Scanning Electron Microscope (Quanta 400F Field Emission, SEM ) at Central Laboratory of Middle East Technical University (Ankara, TURKEY).…”

“…After biofilm formation and co-incubation assays, each of the coupons was removed from the well and washed by sterile PBS three times to remove the medium and nonadhered cells. Following the fixation of the coupons at room temperature in 4% formaldehyde for 3 hr, the washing step was repeated and the coupons were dehydrated in serial dilutions of 30, 50, 60, 70, and 90% ethanol for 5 min each, followed by three rinses for 10 min in 100% ethanol (Kiran et al, 2015).…”

Selection and characterization of broad‐spectrum antibacterial substance‐producing Lactobacillus curvatus PA40 as a potential probiotic for feed additives

“…Although in vitro testing followed by clinical experiments is often employed to evaluate beneficial properties of LAB, current experimental methods used to test resistance to gastric and bile acids, as well as those used to select potential probiotic strains, often fail to mimic in situ conditions (the gastrointestinal environment) (Baik, ). In order to overcome this limitation, an in silico method of testing (conducted before clinical testing and which involves the development of a model similar to the gastrointestinal tract) has recently gained attention (Kiran, Mokrani, & Osmanagaoglu, ). Human gastrointestinal models, such as the stomach and intestinal model developed by TNO Nutrition and Food Research in the Netherlands, Belgium's simulator of the human intestinal microbial ecosystem (SHIME), and DePaola's digestive system in the USA, have been developed and utilized in a wide variety of fields, including LAB‐related research and testing of the bioavailability of bioactive substances (Gardiner, Stanton, Lynch, & Collins, ).…”

Isolation and Probiotic Potential of Lactobacillus Salivarius and Pediococcus Pentosaceus in Specific Pathogen Free Chickens

“…Increasing evidence indicates that Lactobacillus and Pediococcus strains exert beneficial effects by a variety of complementary mechanisms, including an ability to modulate host immune function, high adhesion capacity, cancerpreventing attributes, reduction of serum cholesterol levels, high antimicrobial activity against pathogens, and normalization of the microbiota composition (Okamoto et al, 2007;Noujaim et al, 2009; Isolation and Probiotic Potential of Lactobacillus salivarius and Pediococcus pentosaceus in Specific Pathogen Free Chickens Meng et al, 2010;Zhang et al, 2011;Kiran et al, 2015;Xie et al, 2015;Zhang et al, 2015). Promising probiotic strains should have some superior biological properties, especially improving growth performance, intestinal microbiota balance, and meat quality, and decreasing noxious gas emission (Meng et al, 2010;Fan et al, 2013;Jeong et al, 2014;Peng et al, 2016).…”

“…Pediococcus pentosaceus and Lactobacillus salivarius are most frequently isolated from the duodenal mucosa of piglets (Zhang et al, 2011;Zhang et al, 2015) and human breast milk (Osmanagaoglu et al, 2010;Kiran et al, 2015), but little information is available about the isolated strains from animals and their bioavailability. Therefore, the objectives of this study were to isolate and identify P. pentosaceus and L. salivarius strains from cecal content and investigate their potential probiotic effects on growth, meat quality and microenvironment in specific pathogen free (SPF) chickens, and ammonia treatment in vitro.…”