Effect of Empagliflozin on Sphingolipid Catabolism in Diabetic and Hypertensive Rats

Pérez-Villavicencio, Roxana; Flores‐Estrada, Javier; Franco, Martha; Escalante, Bruno; Pérez-Méndez, Óscar; Mercado, Adriana; Bautista-Pérez, Rocı́o

doi:10.3390/ijms23052883

Cited by 12 publications

(15 citation statements)

References 68 publications

(76 reference statements)

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“…Male Wistar rats (300–350 g) were divided into two groups (n = 10 each): (1) control and (2) streptozotocin (STZ)-induced diabetic rats. The animals with blood glucose of 300 mg/dl (18 mmol/L) were used as diabetic rats and maintained for 30 days, as we previously reported [ 71 ].…”

Section: Methodsmentioning

confidence: 99%

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SARS-CoV-2 Spike Protein Enhances Carboxypeptidase Activity of Angiotensin-Converting Enzyme 2

Mendiola-Salazar,

Munguía-Laguna,

Franco

et al. 2024

IJMS

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In this study, we investigated whether severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein may modify angiotensin-converting enzyme 2 (ACE2) activity in the plasma, heart, kidney, liver, lung, and six brain regions (amygdala, brain stem, cortex, hippocampus, hypothalamus, and striatum) of diabetic and hypertensive rats. We determine ACE2 activity in the plasma and lysates of heart, kidney, liver, lung, and six brain regions. MLN-4760 inhibits ACE2 activity in the plasma and all organs. On the other hand, soluble ACE2 (sACE2) activity increased in the plasma of diabetic rats, and there was no change in the plasma of hypertensive rats. ACE2 activity was augmented in the liver, brain stem, and striatum, while it decreased in the kidney, amygdala, cortex, and hippocampus of diabetic rats. ACE2 activity increased in the kidney, liver, and lung, while it decreased in the heart, amygdala, cortex, and hypothalamus of hypertensive rats. We measured the ACE2 content via enzyme-linked immunosorbent assay and found that ACE2 protein levels increased in the heart, while it decreased in the plasma, kidney, brain stem, cortex, hippocampus, hypothalamus, and striatum of diabetic rats. ACE2 protein levels decreased in the brain stem, cortex, hippocampus, and hypothalamus of hypertensive rats. Our data showed that the spike protein enhanced ACE2 activity in the liver and lungs of diabetic rats, as well as in the heart and three of the brain regions (cortex, hypothalamus, and striatum) of hypertensive rats.

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Section: Methodsmentioning

confidence: 99%

“…Male Wistar rats (350–360 g) were divided into two groups (n = 10 each): (1) control and (2) hypertensive rats (435 ng/kg/min of Ang II, which was infused through osmotic minipumps for 14 days (Alzet 2002; Alza, Palo Alto, CA, USA), as we previously reported [ 71 ].…”

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 Spike Protein Enhances Carboxypeptidase Activity of Angiotensin-Converting Enzyme 2

Mendiola-Salazar,

Munguía-Laguna,

Franco

et al. 2024

IJMS

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“…A single study has shown that ceramide-induced vasodilation is partially endothelium-dependent via activation of NOS3 and subsequent NO generation, as aforementioned, by impairing endothelium function ( 79 ). Angiotensin II (ANGII) may indirectly increase ceramide production, thereby activating SMases in the peripheral vasculature to control vascular function ( 80 ).…”

Section: Ceramides and Vesselsmentioning

confidence: 99%

Role of ceramides in diabetic foot ulcers (Review)

et al. 2023

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diabetes mellitus (dM) is a metabolic disorder, which if not managed properly, can lead to serious health problems over time and impose significant financial burden on the patient, their family and society as a whole. The study of this disease and the underlying biological mechanism is gaining momentum. Multiple pieces of conclusive evidence show that ceramides are involved in the occurrence and development of diabetes. The present review focuses on the function of ceramides, a type of sphingolipid signaling molecule, to provide a brief description of ceramides and their metabolism, discuss the significant roles of ceramides in the healthy skin barrier, and speculate on the potential involvement of ceramides in the pathogenesis and development of diabetic foot ulcers (dFUs). Understanding these aspects of this disease more thoroughly is crucial to establish how ceramides contribute to the etiology of diabetic foot infections and identify possible therapeutic targets for the treatment of dFUs. Contents1. Introduction 2. Biosynthesis and degradation of ceramides 3. Ceramides in the skin 4. ceramides and dFUs 5. ceramides and vessels 6. ceramides and diabetes 7. ceramides and atherosclerosis 8. Mechanism of ceramides in diabetes 9. Mechanism of ceramides in atherosclerosis 10. Shared mechanisms between diabetic complications and dFUs 11. conclusions

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“…Estas permiten identificar y cuantificar algunos lípidos mediante el uso de anticuerpos monoclonales o de colorantes fluorescentes [100][101][102] . Recientemente reportamos que con la técnica de ELISA (Enzyme-Linked-Immunosorbent Assay) es posible determinar la concentración de ceramida, esfingosina y esfingosina-1-fosfato en plasma y tejidos 103 , lo cual permitirá en un futuro aplicarlo en la clínica.…”

Section: Métodos Para El Estudio De Los Esfingolípidos Y Sus Metabolitosunclassified

Metabolismo de los esfingolípidos y su relación con las enfermedades cardiovasculares, renales y metabólicas

Hernández-Bello¹,

Franco²,

Pérez-Méndez³

et al. 2023

ACM

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Los esfingolípidos (esfingomielina, glucolípidos y gangliósidos) se localizan en las membranas celulares, el plasma y las lipoproteínas. En pacientes con enfermedades cardiovasculares, renales y metabólicas, el perfil de los esfingolípidos y sus metabolitos (ceramida, esfingosina y esfingosina-1-fosfato) se modifica, y estos cambios pueden explicar las alteraciones en algunas respuestas celulares, como la apoptosis. Además, se ha sugerido que la esfingosina y la esfingosina-1-fosfato previenen la COVID-19. En esta revisión también se mencionan brevemente las técnicas que permiten el estudio de los esfingolípidos y sus metabolitos.

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Effect of Empagliflozin on Sphingolipid Catabolism in Diabetic and Hypertensive Rats

Cited by 12 publications

References 68 publications

SARS-CoV-2 Spike Protein Enhances Carboxypeptidase Activity of Angiotensin-Converting Enzyme 2

SARS-CoV-2 Spike Protein Enhances Carboxypeptidase Activity of Angiotensin-Converting Enzyme 2

Role of ceramides in diabetic foot ulcers (Review)

Metabolismo de los esfingolípidos y su relación con las enfermedades cardiovasculares, renales y metabólicas

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