Effect of electrode geometry on dielectrophoretic separation of cells

Wakizaka, Yoshikazu; Hakoda, Masaru; Shiragami, Naohiro

doi:10.1016/j.bej.2004.03.002

Cited by 35 publications

(13 citation statements)

References 13 publications

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“…A bioparticles' DEP behavior can be explored by employing either direct current (DC) or alternating current (AC) or both signals simultaneously to generate non-uniform electric fields [11][12][13][14][15][16][17]. Classical DEP utilizes embedded metal electrodes and AC electrical signals to create electric field non-uniformities [1,18,19]. The use of embedded metallic electrodes can catalyze electrochemical reactions [20] potentially altering the local medium to be harmful to the bioparticles.…”

Section: Introductionmentioning

confidence: 99%

Direct current insulator‐based dielectrophoretic characterization of erythrocytes: ABO‐Rh human blood typing

Srivastava¹,

Artemiou²,

Minerick³

2011

Electrophoresis

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A microfluidic platform developed for quantifying the dependence of erythrocyte (red blood cell, RBC) responses by ABO-Rh blood type via direct current insulator dielectrophoresis (DC-iDEP) is presented. The PDMS DC-iDEP device utilized a 400 x 170 μm² rectangular insulating obstacle embedded in a 1.46-cm long, 200-μm wide inlet channel to create spatial non-uniformities in direct current (DC) electric field density realized by separation into four outlet channels. The DC-iDEP flow behaviors were investigated for all eight blood types (A+, A-, B+, B-, AB+, AB-, O+, O-) in the human ABO-Rh blood typing system. Three independent donors of each blood type, same donor reproducibility, different conductivity buffers (0.52-9.1 mS/cm), and DC electric fields (17.1-68.5 V/cm) were tested to investigate separation dependencies. The data analysis was conducted from image intensity profiles across inlet and outlet channels in the device. Individual channel fractions suggest that the dielectrophoretic force experienced by the cells is dependent on erythrocyte antigen expression. Two different statistical analysis methods were conducted to determine how distinguishable a single blood type was from the others. Results indicate that channel fraction distributions differ by ABO-Rh blood types suggesting that antigens present on the erythrocyte membrane polarize differently in DC-iDEP fields. Under optimized conductivity and field conditions, certain blind blood samples could be sorted with low misclassification rates.

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Section: Introductionmentioning

confidence: 99%

Direct current insulator‐based dielectrophoretic characterization of erythrocytes: ABO‐Rh human blood typing

Srivastava¹,

Artemiou²,

Minerick³

2011

Electrophoresis

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“…DEP velocity of cells is derived at the equilibrium of forces. In a horizontal plane, the velocity component of cells moving in laminar medium with low Reynolds number is derived at the equilibrium of DEP force and drag forces [45].…”

Section: Theorymentioning

confidence: 99%

Analytical solutions and validation of electric field and dielectrophoretic force in a bio‐microfluidic channel

et al. 2012

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In a microbiological device, cell or particle manipulation and characterization require the use of electric field on different electrodes in several configurations and shapes. To efficiently design microelectrodes within a microfluidic channel for dielectrophoresis focusing, manipulation and characterization of cells, the designer will seek the exact distribution of the electric potential, electric field and hence dielectrophoresis force exerted on the cell within the microdevice. In this paper we describe the approach attaining the analytical solution of the dielectrophoretic force expression within a microchannel with parallel facing same size electrodes present on the two faces of channel substrates, with opposite voltages on the pair electrodes. Simple Fourier series mathematical expressions are derived for electric potential, electric field and dielectric force between two distant finite-size electrodes. Excellent agreement is found by comparing the analytical results calculated using MATLAB™ with numerical ones obtained by Comsol. This analytical result can help the designer to perform simple design parametric analysis. Bio-microdevices are also designed and fabricated to illustrate the theoretical solution results with the experimental data. Experiments with red blood cells show the dielectrophoretic force contour plots of the analytical data matched to the experimental results.

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“…Channel side wall is slanted to facilitate particles to move towards the focusing area. Due to fabrication constraints the channel side wall angle is kept under 15 degrees (Wakizaka et al 2004). Dielectric structure is a four sided polygon extruded along the length of the channel.…”

Section: Geometry Improvementmentioning

confidence: 99%

“…Due to these constraints the device is difficult to manufacture and sometimes cannot be used commercially due to its cost effectiveness. Dielectrophoresis (DEP) provides an attractive alternative to conventional methods because of its ability to concentrate and separate microorganisms in a selective, rapid, and reversible manner (Pohl 1951(Pohl , 1978Jones 1995;Cummings and Singh 2003;Doh and Cho 2005). Concentrating an electric field at the center of the channel by means of dielectric separated by the electrodes induces the non-uniform electric field gradient across the cross-section of the channel.…”

Section: Introductionmentioning

confidence: 99%

Numerical study of particle focusing through improved Lab-on-a-Chip device by positive dielectrophoresis

et al. 2009

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In this paper an improved Lab-on-a-Chip device has been proposed for focusing the particle at the center of the channel to separate the desired particles from the main stream at the exit. The design methodology is kept simple and a two fluidic port device with geometric description is proposed. The induced dielectric force responsible for focusing particle is accomplished by means of two electrodes separated by dielectric material. Compared to the previous particle focusing devices; this device provides simple and effective mean of particle focusing with numerical study highlighting the importance of geometric improvements and efficiency.

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Effect of electrode geometry on dielectrophoretic separation of cells

Cited by 35 publications

References 13 publications

Direct current insulator‐based dielectrophoretic characterization of erythrocytes: ABO‐Rh human blood typing

Direct current insulator‐based dielectrophoretic characterization of erythrocytes: ABO‐Rh human blood typing

Analytical solutions and validation of electric field and dielectrophoretic force in a bio‐microfluidic channel

Numerical study of particle focusing through improved Lab-on-a-Chip device by positive dielectrophoresis

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