Analytical solutions and validation of electric field and dielectrophoretic force in a bio‐microfluidic channel

Nerguizian, Vahé; Alazzam, Anas; Roman, D.; Stiharu, Ion; Burnier, Miguel N

doi:10.1002/elps.201100325

Cited by 19 publications

(18 citation statements)

References 49 publications

(31 reference statements)

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“…AC‐based DEP requires nonuniform electric field which, in general, is created using conductive electrodes . The DEP force on a spherical polarizable microentity under nonuniform electric field while immersed in a conductive medium is defined by :

F_{DEP} = 2 π ε_{normalm} r^{3} Re [f_{cm}] \nabla (E_{rms}^{2})

where ε m is the permittivity of the medium, r is the radius of the microentity, E is the electric field, and Re[ f CM ] is the real part of Clausius–Mossotti factor that depends on the complex permittivities of the microentity and the suspended medium and is defined as:

f_{cm} = \frac{ε_{p}^{*} - ε_{m}^{*}}{ε_{p}^{*} + 2 ε_{m}^{*}}

where ε * is the complex permittivity that is a function of the dielectric constant ε , the angular frequency ω , and the real conductivity σ and is given by:

ε^{*} = ε - j \frac{σ}{ω}

where j is the imaginary unit,

j = \sqrt{- 1}

…”

Section: Methodsmentioning

confidence: 99%

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

2017

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We describe the design, microfabrication, and testing of a microfluidic device for the separation of cancer cells based on dielectrophoresis. Cancer cells, specifically green fluorescent protein-labeled MDA-MB-231, are successfully separated from a heterogeneous mixture of the same and normal blood cells. MDA-MB-231 cancer cells are separated with an accuracy that enables precise detection and counting of circulating tumor cells present among normal blood cells. The separation is performed using a set of planar interdigitated transducer electrodes that are deposited on the surface of a glass wafer and slightly protrude into the separation microchannel at one side. The device includes two parts, namely, a glass wafer and polydimethylsiloxane element. The device is fabricated using standard microfabrication techniques. All experiments are conducted with low conductivity sucrose-dextrose isotonic medium. The variation in response between MDA-MB-231 cancer cells and normal cells to a certain band of alternating-current frequencies is used for continuous separation of cells. The fabrication of the microfluidic device, preparation of cells and medium, and flow conditions are detailed. The proposed microdevice can be used to detect and separate malignant cells from heterogeneous mixture of cells for the purpose of early screening for cancer.

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F_{DEP} = 2 π ε_{normalm} r^{3} Re [f_{cm}] \nabla (E_{rms}^{2})

f_{cm} = \frac{ε_{p}^{*} - ε_{m}^{*}}{ε_{p}^{*} + 2 ε_{m}^{*}}

where ε * is the complex permittivity that is a function of the dielectric constant ε , the angular frequency ω , and the real conductivity σ and is given by:

ε^{*} = ε - j \frac{σ}{ω}

where j is the imaginary unit,

j = \sqrt{- 1}

…”

Section: Methodsmentioning

confidence: 99%

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

2017

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“…The electric potential inside the microchannel is described using the Laplace equation, Eq. , and is based on the assumption that there are no electric charges inside the microchannel .

Δ V = 0 where V (x, z) = f (V_{normalo}, D_{ch}, d_{normale}, g_{normale})

where V ( V ) is the electric potential, V o ( V ) is the actuation voltage, D ch (m or μm) is the microchannel depth, d e (m or μm) is the electrode length, and g e (m or μm) is the electrode gap length.…”

Section: Theoretical Modelmentioning

confidence: 99%

Model‐based analysis of a dielectrophoretic microfluidic device for field‐flow fractionation

Mathew

Alazzam

Abutayeh

et al. 2016

J of Separation Science

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We present the development of a dynamic model for predicting the trajectory of microparticles in microfluidic devices, employing dielectrophoresis, for Hyperlayer field-flow fractionation. The electrode configuration is such that multiple finite-sized electrodes are located on the top and bottom walls of the microchannel; the electrodes on the walls are aligned with each other. The electric potential inside the microchannel is described using the Laplace equation while the microparticles' trajectory is described using equations based on Newton's second law. All equations are solved using finite difference method. The equations of motion account for forces including inertia, buoyancy, drag, gravity, virtual mass, and dielectrophoresis. The model is used for parametric study; the geometric parameters analyzed include microparticle radius, microchannel depth, and electrode/spacing lengths while volumetric flow rate and actuation voltage are the two operating parameters considered in the study. The trajectory of microparticles is composed of transient and steady state phases; the trajectory is influenced by all parameters. Microparticle radius and volumetric flow rate, above the threshold, do not influence the steady state levitation height; microparticle levitation is not possible below the threshold of the volumetric flow rate. Microchannel depth, electrode/spacing lengths, and actuation voltage influence the steady-state levitation height.

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“…As the force due to DEP depends on the electric field, the electrode configurations that generate the electric field need to be given significant consideration while designing DEP-FFF microdevices. To this extend researchers have envisioned and analyzed several electrode configurations (Alazzam et al, 2010;Nerguizian et al, 2012;Nieuwenhuis and Vellekoop, 2004). The force due to DEP associated with each electrode configuration is unique and consequently the trajectory of microparticles in a DEP-FFF microdevice would also be unique.…”

Section: Introductionmentioning

confidence: 99%

Modeling the trajectory of microparticles subjected to dielectrophoresis in a microfluidic device for field flow fractionation

Mathew

Alazzam

Abutayeh

et al. 2015

Chemical Engineering Science

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Analytical solutions and validation of electric field and dielectrophoretic force in a bio‐microfluidic channel

Cited by 19 publications

References 49 publications

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

Model‐based analysis of a dielectrophoretic microfluidic device for field‐flow fractionation

Modeling the trajectory of microparticles subjected to dielectrophoresis in a microfluidic device for field flow fractionation

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