Effect of electricity on microbial community of microbial fuel cell simultaneously treating sulfide and nitrate

Cai, Jing; Zheng, Ping; Xing, Yajuan; Mahmood, Qaisar

doi:10.1016/j.jpowsour.2015.01.165

Cited by 29 publications

(13 citation statements)

References 35 publications

(33 reference statements)

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“…Its abundance was less than 2% in all other samples. Strictly anaerobic and nitrate-reducing species of Vulcanibacillus genus were previously reported in electroactive biofilm [46]. In this study, its presence was higher in SC cathodic biofilm (3.4%) than in SM terracotta biofilm (1.9%).…”

Section: Figuresupporting

confidence: 47%

A study of microbial communities on terracotta separator and on biocathode of air breathing microbial fuel cells

Rago¹,

Zecchin

Marzorati³

et al. 2018

Bioelectrochemistry

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Recently, terracotta has attracted interest as low-cost and biocompatible material to build separators in microbial fuel cells (MFCs). However, the influence of a non-conductive material like terracotta on electroactive microbiological communities remains substantially unexplored. This study aims at describing the microbial pools developed from two different seed inocula (bovine and swine sewage) in terracotta-based air-breathing MFC. A statistical approach on microbiological data confirmed different community enrichment in the MFCs, depending mainly on the inoculum. Terracotta separators impeded the growth of electroactive communities in contact with cathodes (biocathodes), while a thick biofilm was observed on the surface (anolyte-side) of the terracotta separator. Terracotta-free MFCs, set as control experiments, showed a well-developed biocathode, Biocathode-MFCs resulted in 4 to 6-fold higher power densities. All biofilms were analyzed by high-throughput Illumina sequencing applied to 16S rRNA gene. The results showed more abundant (3- to 5-fold) electroactive genera (mainly Geobacter, Pseudomonas, Desulfuromonas and Clostridia MBA03) in terracotta-free biocathodes. Nevertheless, terracotta separators induced only slight changes in anodic microbial communities.

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Section: Figuresupporting

confidence: 47%

A study of microbial communities on terracotta separator and on biocathode of air breathing microbial fuel cells

Rago¹,

Zecchin

Marzorati³

et al. 2018

Bioelectrochemistry

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“…Geobacter and Rhodopseudomonas genera and the Rhodocyclaceae family (close to Azoarcus and Thauera genera) were the most numerically abundant bacteria in RNA library, along with ∼10% population of Azospirillum and Aeromonas genera. The literature has reported isolation of or genomic dominance by Geobacter , Rhodopseudomonas , Azoarcus , Thauera , Azospirillum , and Aeromonas genera in biofilm anodes. − Except for the Geobacter genus, these ARB produce low to moderate ranges of current density, from 0.3 to 5 A/m 2 in acetate-fed MxCs ,,,− or MxCs run with simple organics (C1 to C3). ,, Thus, our biofilm anode was dominated by known ARB, but Geobacter was only 27% (DNA) or 21% (RNA). We cannot completely exclude that a small amount of O 2 may have intruded into the anode chamber of the MxC, perhaps through the feeding tubing.…”

Section: Resultsmentioning

confidence: 78%

The Roles of Biofilm Conductivity and Donor Substrate Kinetics in a Mixed-Culture Biofilm Anode

Lee

Dhar

et al. 2016

Environ. Sci. Technol.

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We experimentally assessed the kinetics and thermodynamics of electron transfer (ET) from the donor substrate (acetate) to the anode for a mixed-culture biofilm anode. We interpreted the results with a modified biofilm-conduction model consisting of three ET steps in series: (1) intracellular ET, (2) non-Ohmic extracellular ET (EET) from an outer membrane protein to an extracellular cofactor (EC), and (3) ET from the EC to the anode by Ohmic-conduction in the biofilm matrix. The steady-state current density was 0.82 ± 0.03 A/m in a miniature microbial electrochemical cell operated at fixed anode potential of -0.15 V versus the standard hydrogen electrode. Illumina 16S-rDNA and -rRNA sequences showed that the Geobacter genus was less than 30% of the community of the biofilm anode. Biofilm conductivity was high at 2.44 ± 0.42 mS/cm, indicating that the maximum current density could be as high as 270 A/m if only Ohmic-conduction EET was limiting. Due to the high biofilm conductivity, the maximum energy loss for Ohmic-conduction EET was negligible, 0.085 mV. The energy loss in the second ET step also was small, only 20 mV, and the potential for the EC involved in the second ET was -0.15 V, a value documenting that >99% of the EC was in the oxidized state. Monod kinetics for utilization of acetate were relatively slow, and at least 87% of the energy loss was in the intracellular step. Thus, intracellular ET was the main kinetic and thermodynamic bottleneck to ET from donor substrate to the anode for a highly conductive biofilm.

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“…Shannon and PD whole tree indexes contain information about the richness and evenness of bacterial communities. 29 The bacterial diversity evaluated by the Shannon estimator also indicated that MFC 4 had the highest microbial diversity. According to the α diversity analysis, the response of the bacterial communities to diverse medicinal plant EMs is different.…”

Section: ■ Results and Discussionmentioning

confidence: 97%

“…Based on the two indexes above, adding herbal plants into MFC 2 , MFC 3 , MFC 5 , and MFC 6 reduced the richness of the microbial community in the anode biofilm compared with MFC 1 , while the richness of MFC 4 with B. rheum addition was higher than that of the open group and other closed groups. Shannon and PD whole tree indexes contain information about the richness and evenness of bacterial communities . The bacterial diversity evaluated by the Shannon estimator also indicated that MFC 4 had the highest microbial diversity.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Performance of Microbial Fuel Cells with Electron Mediators from Anthraquinone/Polyphenol-Abundant Herbal Plants

Yang

Chen

et al. 2020

ACS Sustainable Chem. Eng.

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The limited amount of electron mediators (EMs) excreted by microorganisms has restricted electricity generation and the related pollutant removal in microbial fuel cells (MFCs). The polyphenolic-rich plants contain simple polyphenols or anthraquinones that have promising electron-shuttling potential, but this has not been well understood. Herein, four herbal plants Polygonum multiflorum (T. fallopia), rhubarb (B. rheum), radix rubiae (L. Rubia) and semen cassiae (Catsia tora Linn) were selected and then studied to produce EMs to stimulate electricity generation in MFCs. B. rheum had the highest redox activity and 2% acid pretreatment contributing the most to the release of electroactive substances. The highest power density (18.67 W/m 3 ) and Coulombic efficiency (29.03%) and the lowest internal resistance (29.02 Ω) were achieved in MFCs with B. rheum addition compared to other herbal plants. The satisfactory COD (93.68%) and NH 4 + -N (39.68%) removal were also obtained with a 0.01 g/L dosage. Confocal laser scanning microscopy and high throughput sequencing analysis showed that B. rheum had the least negative effects on biofilm microstructure and microbial species, corresponding to its outstanding performance. These findings first suggested that B. rheum can be regarded as a promising redox mediator to improve MFC performance. This study provided a new thinking to apply herbal wastewater or pharmaceutical waste in improving the bioelectrochemical system.

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Effect of electricity on microbial community of microbial fuel cell simultaneously treating sulfide and nitrate

Cited by 29 publications

References 35 publications

A study of microbial communities on terracotta separator and on biocathode of air breathing microbial fuel cells

A study of microbial communities on terracotta separator and on biocathode of air breathing microbial fuel cells

The Roles of Biofilm Conductivity and Donor Substrate Kinetics in a Mixed-Culture Biofilm Anode

Enhanced Performance of Microbial Fuel Cells with Electron Mediators from Anthraquinone/Polyphenol-Abundant Herbal Plants

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