Effect of electrical stimulation on the viability of the hippocampal slice preparation

Schurr, Avital; Reid, Kenneth H.; Tseng, Michael T.; Edmonds, H. L.; West, Catherine A.; Rigor, Benjamin M.

doi:10.1016/0361-9230(86)90045-6

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…Consequently, the PS amplitude measured during the exposure to Glut quickly falls to 0 mV, only to fully recover after 30 min of Glut washout under control conditions (see samples of PS traces in Figure 1). In contrast, high frequency electrical stimulation had a much less dramatic effect on the PS amplitude, as we have shown previously (Schurr et al, 1986). In each paradigm, the effect of blocking lactate transport with the monocarboxylate transporter (MCT) inhibitor α-cyano-4-hydroxycinnamate (4-CIN, Halestrap and Denton, 1975) was assessed.…”

Section: Resultssupporting

confidence: 78%

Aerobic production and utilization of lactate satisfy increased energy demands upon neuronal activation in hippocampal slices and provide neuroprotection against oxidative stress

Schurr

Gozal

2011

fphar

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Ever since it was shown for the first time that lactate can support neuronal function in vitro as a sole oxidative energy substrate, investigators in the field of neuroenergetics have been debating the role, if any, of this glycolytic product in cerebral energy metabolism. Our experiments employed the rat hippocampal slice preparation with electrophysiological and biochemical methodologies. The data generated by these experiments (a) support the hypothesis that lactate, not pyruvate, is the end-product of cerebral aerobic glycolysis; (b) indicate that lactate plays a major and crucial role in affording neural tissue to respond adequately to glutamate excitation and to recover unscathed post-excitation; (c) suggest that neural tissue activation is accompanied by aerobic lactate and NADH production, the latter being produced when the former is converted to pyruvate by mitochondrial lactate dehydrogenase (mLDH); (d) imply that NADH can be utilized as an endogenous scavenger of reactive oxygen species (ROS) to provide neuroprotection against ROS-induced neuronal damage.

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Section: Resultssupporting

confidence: 78%

Aerobic production and utilization of lactate satisfy increased energy demands upon neuronal activation in hippocampal slices and provide neuroprotection against oxidative stress

Schurr

Gozal

2011

fphar

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“…Also importantly, test stimulation activates NMDA receptors and the molecular cascades linked to it . Minimizing the impact of test stimulation is in the interest of recording stability (Schurr et al, 1986) as well as reproducibility of the data from experiment to experiment. Data (fEPSP slope) was normalized to a baseline calculated over 60 min before the first tetanization.…”

Section: Methodsmentioning

confidence: 99%

Synaptic Tagging and Capture: Differential Role of Distinct Calcium/Calmodulin Kinases in Protein Synthesis-Dependent Long-Term Potentiation

Redondo¹,

Okuno²,

Spooner³

et al. 2010

J. Neurosci.

168

140

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Weakly tetanized synapses in area CA1 of the hippocampus that ordinarily display long-term potentiation lasting ϳ3 h (called early-LTP) will maintain a longer-lasting change in efficacy (late-LTP) if the weak tetanization occurs shortly before or after strong tetanization of an independent, but convergent, set of synapses in CA1. The synaptic tagging and capture hypothesis explains this heterosynaptic influence on persistence in terms of a distinction between local mechanisms of synaptic tagging and cell-wide mechanisms responsible for the synthesis, distribution, and capture of plasticity-related proteins (PRPs). We now present evidence that distinct CaM kinase (CaMK) pathways serve a dissociable role in these mechanisms. Using a hippocampal brain-slice preparation that permits stable long-term recordings in vitro for Ͼ10 h and using hippocampal cultures to validate the differential drug effects on distinct CaMK pathways, we show that tag setting is blocked by the CaMK inhibitor KN-93 (2-[N-(2-hydroxyethyl)]-N-(4-methoxybenzenesulfonyl)amino-N-(4-chlorocinnamyl)-N-methylbenzylamine) that, at low concentration, is more selective for CaMKII. In contrast, the CaMK kinase inhibitor STO-609 [7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylic acid] specifically limits the synthesis and/or availability of PRPs. Analytically powerful three-pathway protocols using sequential strong and weak tetanization in varying orders and test stimulation over long periods of time after LTP induction enable a pharmacological dissociation of these distinct roles of the CaMK pathways in late-LTP and so provide a novel framework for the molecular mechanisms by which synaptic potentiation, and possibly memories, become stabilized.

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“…All studies were approved by our Institutional Animal Care and Use Committee. The homemade hypoxic device (referring to Vannucci’s and other methods[ 4 ]) consisted of a noninvasive vascular folder of 8% nitrogen-oxygen mixed gas (containing 8% oxygen and 92% nitrogen, provided by the Nanjing Flextronics Gas Co., Ltd.). Hypoxia equipment consisted of a high pressure tank filled with nitrogen-oxygen mixed gas, a 5-L sealed hypoxic disposal tank, an outflow of gas, water bottle, mask, oxygen valve, an oxygen flow metre, and connection tubes.…”

Section: Methodsmentioning

confidence: 99%

“…Non-lethal hypoxic preconditioning (HP) can increase tolerance to I/R injury and is effective in reducing damage to a variety of organs[ 4 ], including the liver and kidney[ 5 ]. For tumour cells, research has found that HIF-1α plays an important role in hypoxia conditioning, and HIF-1α is also the critical transcription factor that mediates cell hypoxia reactions[ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia preconditioning protects Ca²⁺-ATPase activation of intestinal mucosal cells against R/I injury in a rat liver transplantation model

Shi

Zhang

et al. 2018

WJG

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AIMTo investigate the effect of ischaemia and reperfusion (I/R) injury on the Ca2+-ATPase activation in the intestinal tissue of a rat autologous orthotopic liver transplantation model and to determine if hypoxia preconditioning (HP) therapy induces HIF-1α to protect rat intestinal tissue against I/R injury.METHODSRats received non-lethal hypoxic preconditioning therapy to induce HIF-1α expression. We used an autologous orthotopic liver transplantation model to imitate the I/R injury in intestinal tissue. Then, we detected the microstructure changes in small intestinal tissues, Ca2+-ATPase activity, apoptosis, and inflammation within 48 h postoperatively.RESULTSHIF-1α expression was significantly increased in intestinal tissue at 12 h postoperatively in rats that were exposed to a hypoxic environment for 90 min compared with a non-HP group (HP vs AT, P = 0.0177). Pathological analysis was performed on the intestinal mucosa cells, and the cells in the HP group appeared healthier than the cells in the AT group. The Ca2+-ATPase activity in the small intestinal cells in the AT group was significantly lower after the operation, and the Ca2+-ATPase activity in the HP group recovered faster than that in the AT group at 6 h postoperatively (HP vs AT, P = 0.0106). BCL-2 expression in the HP group was significantly higher than that in the AT group at 12 h postoperatively (HP vs AT P = 0.0010). The expression of the inflammatory factors NO, SOD, IL-6, and TNF-α was significantly lower in the HP group than in the AT group.CONCLUSIONHypoxia-induced HIF-1α could protect intestinal mucosal cells against mitochondrial damage after I/R injury. HP could improve hypoxia tolerance in small intestinal mucosal cells and increase Ca2+-ATPase activity to reduce the apoptosis of and pathological damage to intestinal cells. HP could be a useful way to promote the earlier recovery of intestinal function after graft procedure.

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Effect of electrical stimulation on the viability of the hippocampal slice preparation

Cited by 10 publications

References 12 publications

Aerobic production and utilization of lactate satisfy increased energy demands upon neuronal activation in hippocampal slices and provide neuroprotection against oxidative stress

Aerobic production and utilization of lactate satisfy increased energy demands upon neuronal activation in hippocampal slices and provide neuroprotection against oxidative stress

Synaptic Tagging and Capture: Differential Role of Distinct Calcium/Calmodulin Kinases in Protein Synthesis-Dependent Long-Term Potentiation

Hypoxia preconditioning protects Ca²⁺-ATPase activation of intestinal mucosal cells against R/I injury in a rat liver transplantation model

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Effect of electrical stimulation on the viability of the hippocampal slice preparation

Cited by 10 publications

References 12 publications

Aerobic production and utilization of lactate satisfy increased energy demands upon neuronal activation in hippocampal slices and provide neuroprotection against oxidative stress

Aerobic production and utilization of lactate satisfy increased energy demands upon neuronal activation in hippocampal slices and provide neuroprotection against oxidative stress

Synaptic Tagging and Capture: Differential Role of Distinct Calcium/Calmodulin Kinases in Protein Synthesis-Dependent Long-Term Potentiation

Hypoxia preconditioning protects Ca2+-ATPase activation of intestinal mucosal cells against R/I injury in a rat liver transplantation model

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Hypoxia preconditioning protects Ca²⁺-ATPase activation of intestinal mucosal cells against R/I injury in a rat liver transplantation model