Effect of egg yolk levels and equilibration periods on freezability of Jamunapari buck semen

Ranjan, Ravi; Goel, Anjana; Ramachandran, N.; Kharche, S. D.; Jindal, Shalini

doi:10.5958/0973-9718.2015.00027.6

Cited by 23 publications

(21 citation statements)

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“…The mean post-thaw motility (PTM) and acrosomal integrity was significantly (p<0.01) higher in Group II. The result of PTM of both groups was almost similar to the findings of Tharasanit and Techakumphu [11] in Black Bengal goat but the PTM of semen in Group II bucks was lower than those reported in semi-intensively managed Jamunpari bucks [12]. Although it was higher in Group I than reported by Daskin et al [13] in intensively managed Angora bucks.…”

Section: Resultssupporting

confidence: 86%

Effect of management system and season on semen freezability in Jakhrana bucks

Kumar¹,

Rai²,

Bhat³

et al. 2016

Vet World

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Aim: The objective of the study was to determine the effect of the management system (intensive and semi-intensive) and season (autumn and winter) on semen freezability in Jakhrana bucks. Materials and Methods:A total of 24 Jakhrana bucks of same body weight and age (BW=30 kg, age=1 year) were randomly allotted into two groups, viz., Group I (intensive system, 12 bucks) and Group II (semi-intensive system, 12 bucks). These two groups were statistically tested for their homogeneity with respect to age and BW. Semen was collected twice weekly using an artificial vagina during two seasons: autumn (September-November) and winter (December-February). A total of 240 semen samples (120 from each group and season) were evaluated for post-thaw motility (PTM), viability, abnormality, functional membrane integrity (hypo-osmotic swelling [HOS]) response and acrosomal integrity. Results:The mean values of PTM and acrosomal integrity of spermatozoa were significantly (p<0.01) higher in Group II as compared to Group I. The mean values of viability and abnormality were also differed significant (p<0.05) between groups. However, the mean values of HOS response were found non-significant (p>0.05) between groups. The season showed a significant effect on all parameters except viability and HOS response. The PTM and acrosomal integrity of spermatozoa were significantly (p<0.01) higher in winter as compared to autumn season. Abnormality of spermatozoa was significantly (p<0.05) lower in winter season. Conclusions:This study indicates that both management system and season influence semen freezability. The semen collected from bucks reared under the semi-intensive system and winter season showed better semen freezability characteristics.

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Section: Resultssupporting

confidence: 86%

Effect of management system and season on semen freezability in Jakhrana bucks

Kumar¹,

Rai²,

Bhat³

et al. 2016

Vet World

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“…Average post thaw viability observed in present study (Table 2) was higher than observations in Boer bucks (27.33±0.85), Yodmingkwan et al, (2016); Jamunapari bucks (41.01±3.02), Ramachandran et al, (2015); Gaddi bucks (45.26±1.32), Sharma, (2018) and less than Black Bengal bucks (50.48±0.65), Singh et al,(2016); Jakhrana bucks (53.4±0.80), Priyadharshini et al, (2011); Alpine Sannen and Beetal crosses (55.25-65.75%), Narwade et al, (2017); Chegu bucks (65.20±1.53), Thakur et al, (2005b). Similarly, HOST reactive sperms in present study (Table 2) were higher than observations in Boer bucks (8.04±1.02), Yodmingkwan et al, (2016); Jamunapari bucks (41.01± 3.02), Ramachandran et al, (2015); lower than Black Bengal bucks (47.02±0.58), Singh et al, (2016), Jakhrana bucks (46.45-47.31%), Kumar et al (2016); Jamunapari bucks (51.83±1.9), Ranjan et al,(2015); Gaddi bucks (52.48±1.43), Sharma, (2018) and Alpine Sannen and Beetal crosses (61.0-64.63%), Narwade et al, (2017), respectively.…”

Section: Resultscontrasting

confidence: 53%

Cryopreservation and fertility of frozen thawed Chegu goat semen

Sharma

Sood

2019

IJAR

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Goats are important livestock species of India. Chegu is a pashmina producing goat native to the cold arid region of Himachal Pradesh (H.P.), India. Semen cryopreservation from six Chegu elite males (aged 2.05±0.40 years; weighed 29.16±2.02 kg) was practiced using Tris Citrate Egg Yolk extender containing 10% EY and 6% Glycerol. Gross semen parameters includes volume (0.80.85±0.07 ml), color (Creamy white to yellowish), concentration (2238.5±231.0 x106 spermatozoa/ml) and mass motility (3.92±0.03).The significant changes (P less than 0.01) in post thaw seminal parameters (75.48±0.69 v/s 37.38±0.90; progressive motility), viability (75.79±0.95 v/s 48.25±1.78), morphological abnormalities (5.64±0.29 v/s 7.02±0.32) and HOST reactive spermatozoa (64.07±1.75 v/s 43.35±1.79) were observed in present study. Artificial insemination using frozen thawed semen having concentration (150 x106 spermatozoa/straw) from three different bucks was practiced in 40 synchronized goats with conception rate of 42.5 per cent. Non-significant variations amongst different bucks were observed with birth of 1.12 kids per doe and twinning rate of 11.8 per cent. It was concluded that semen cryopreservation along with artificial insemination can be practiced in Chegu goats to improve the population of this endangered species.

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“…Both these interactions stimulate cryocapacitation, acrosomal damage, loss of motility and viability of sperm (Upreti et al 1999). Therefore, to reduce these interactive losses, protocols that include sperm washing (Roca et al 1997) and use of low or high egg yolk concentration (Bispo et al 2011;Naing et al 2011;Ranjan et al 2015) with variable sperm count have been proposed. Sperm washing removes the protective proteins and antioxidative enzymes while the concentration of egg yolk affects the cryoprotective capacity of the extender (Ferreiral et al 2014), indicating a cumulative effect of different extrinsic and intrinsic factors in regulating the semen quality at different steps during cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

Effect of egg yolk concentration and washing on sperm quality following cryopreservation in Barbari buck semen

Anand

Baghel

Yadav

2016

Journal of Applied Animal Research

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The present study was conducted to evaluate the effect of egg yolk concentration and washing on sperm quality in cryopreserved Barbari buck semen at higher dilution (100 × 10 6 cells/ml). Five healthy Barbari bucks of similar age and weight were selected as semen donor. Six ejaculates were collected from each buck during the experiment. Collected semen samples were pooled and diluted with Tris-based semen extender containing 6% glycerol and egg yolk according to the treatments -T-1, that is, 3% egg yolk, T-2, that is, 20% egg yolk and T-3, that is, 20% egg yolk after washing and cryopreserved using the slow freezing technique. Semen was evaluated at three different steps, namely, after dilution, after equilibration and after freeze-thawing. Significantly (p ≤ .01) higher values for motility and viability were observed in T-3 just after dilution while significantly (p ≤ .01) higher values for acrosomal integrity and pattern F (uncapacitated sperm) was observed in T-2. Significantly (p ≤ .01) higher values were observed in T-2 after equilibration and thawing. It may be concluded that incorporation of 20% egg yolk compared to 3% egg yolk or 20% egg yolk after washing in extender confer better cryoprotection to Barbari buck spermatozoa as reflected in semen quality parameters studied. ARTICLE HISTORY

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Effect of egg yolk levels and equilibration periods on freezability of Jamunapari buck semen

Cited by 23 publications

References 0 publications

Effect of management system and season on semen freezability in Jakhrana bucks

Effect of management system and season on semen freezability in Jakhrana bucks

Cryopreservation and fertility of frozen thawed Chegu goat semen

Effect of egg yolk concentration and washing on sperm quality following cryopreservation in Barbari buck semen

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