Effect of management system and season on semen freezability in Jakhrana bucks

Abstract

Aim: The objective of the study was to determine the effect of the management system (intensive and semi-intensive) and season (autumn and winter) on semen freezability in Jakhrana bucks. Materials and Methods:A total of 24 Jakhrana bucks of same body weight and age (BW=30 kg, age=1 year) were randomly allotted into two groups, viz., Group I (intensive system, 12 bucks) and Group II (semi-intensive system, 12 bucks). These two groups were statistically tested for their homogeneity with respect to age and BW. Semen was collected twice weekly using an artificial vagina during two seasons: autumn (September-November) and winter (December-February). A total of 240 semen samples (120 from each group and season) were evaluated for post-thaw motility (PTM), viability, abnormality, functional membrane integrity (hypo-osmotic swelling [HOS]) response and acrosomal integrity. Results:The mean values of PTM and acrosomal integrity of spermatozoa were significantly (p<0.01) higher in Group II as compared to Group I. The mean values of viability and abnormality were also differed significant (p<0.05) between groups. However, the mean values of HOS response were found non-significant (p>0.05) between groups. The season showed a significant effect on all parameters except viability and HOS response. The PTM and acrosomal integrity of spermatozoa were significantly (p<0.01) higher in winter as compared to autumn season. Abnormality of spermatozoa was significantly (p<0.05) lower in winter season. Conclusions:This study indicates that both management system and season influence semen freezability. The semen collected from bucks reared under the semi-intensive system and winter season showed better semen freezability characteristics.

Cryopreservation and fertility of frozen thawed Chegu goat semen

“…The average morphological abnormalities in post thaw semen of present study (Table 2) corroborated with the findings of Elsheikh and Elhammali, (2015), 4.9-11% during different seasons in Sannen bucks; were lower than those of Sharma, (2018), 7.93±0.28%, Gaddi bucks; Kumar et al, (2016) 12.18-13.73%, Jakhrana bucks; Singh et al, (2016) 13.37-16.81%, Black Bengal bucks; Thakur et al,(2005b) 23.3% Chegu bucks and much higher than those of Yodmingkwan et al, (2016) 1.54-1.71%, Boer bucks; Ramachandran et al, (2015) 2.84±0.49 Jamunapari buck, respectively. The most probable reason for morphological abnormalities seems to be the physical and chemical environments to which a spermatozoa is exposed during the preservation.…”

“…Average post thaw viability observed in present study (Table 2) was higher than observations in Boer bucks (27.33±0.85), Yodmingkwan et al, (2016); Jamunapari bucks (41.01±3.02), Ramachandran et al, (2015); Gaddi bucks (45.26±1.32), Sharma, (2018) and less than Black Bengal bucks (50.48±0.65), Singh et al,(2016); Jakhrana bucks (53.4±0.80), Priyadharshini et al, (2011); Alpine Sannen and Beetal crosses (55.25-65.75%), Narwade et al, (2017); Chegu bucks (65.20±1.53), Thakur et al, (2005b). Similarly, HOST reactive sperms in present study (Table 2) were higher than observations in Boer bucks (8.04±1.02), Yodmingkwan et al, (2016); Jamunapari bucks (41.01± 3.02), Ramachandran et al, (2015); lower than Black Bengal bucks (47.02±0.58), Singh et al, (2016), Jakhrana bucks (46.45-47.31%), Kumar et al (2016); Jamunapari bucks (51.83±1.9), Ranjan et al,(2015); Gaddi bucks (52.48±1.43), Sharma, (2018) and Alpine Sannen and Beetal crosses (61.0-64.63%), Narwade et al, (2017), respectively.…”

“…Average post thaw progressive motility observed in present study (Table 2) was higher than observations in Jamunapari bucks (28.82±1.99), Ramachandran et al, (2015); Blanca de Rasquera bucks (18.2-24.5%); Tabarez et al, (2017); Boer bucks (34.89±0.68), Yodmingkwan et al, (2016); Gaddi bucks (35.18±0.87), Sharma, (2018); Jakhrana bucks (35.2±2.40), Priyadharshini et al,(2011) and less than Jakhrana bucks (40.17-42.79%) Kumar et al, (2016); Black Bengal bucks (40.89±0.65), Singh et al, (2016); Alpine Sannen and Beetal crosses (45-56%), Narwade et al,(2017); Chegu bucks (53.43±1.19), Thakur et al, (2005b); Sannen bucks (47.50-55.63%), Niyazi et al, (2014). Variation among present and other studies on post thaw motility could be due to subjective assessment of progressive motility, in present study only the progressively motile sperms with vigorous linear movement were included.…”

Semen Characteristics of NARI Suwarna Rams during Breeding (Winter) and Non-breeding (Summer) Seasons

“…Further, in complete contrast to the present study, Azawi and Ismaeel (2012) reported seasonal variations with lower sperm concentration and viability with higher acrosomal damages during winter in Awassi rams. Similarly, Kumar et al, (2016) in Jakhrana bucks observed similar post-thaw HOST percentages between the seasons but significantly higher post-thaw acrosome integrity during the winter season. Further, D'Alessandro and Martemucci ( 2003) also observed significantly lower acrosomal damage during the breeding season in boars.…”

Evaluation of lipidomic change in goat sperm after cryopreservation

“…In origin, and making genetic selection of males as an important factor for successful sperm freezing ( 42 ). In previous studies, it attempted to elucidate differences in sperm cryotolerance through investigating marker prediction, management system and cryopreservation technology processes ( 43 , 44 ). Thus, it is the first time to characterize the lipidomic profiles of post-thawed sperm with high and low freezing resistance, and investigate the relationship between the lipids of the sperm and the post-thawing sperm characteristics of goats.…”