Effect of Early Statin Therapy on Circulating Endothelial Progenitor Cells During the Acute Phase in Patients With Acute Myocardial Infarction

Choi, Jun‐Ho; Oh, Seok Kyu; Yun, Kyeong Ho; Shin, Ik Sang; Kim, Seunghwan; Lee, An Saeng; Park, Eun Mi; Rhee, Sang Hoon; Yoo, Nam Jin; Lee, Eun Mi; Kim, Nam-Ho; Jeong, Jaehoon

doi:10.4070/kcj.2008.38.8.411

Cited by 1 publication

(2 citation statements)

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“…In all participants, the total EPC count was assessed by using an in vitro assay, as described previously 8). In brief, mononuclear cells (MNCs) were obtained from peripheral blood samples (100 µL), and the EPCs were identified by flow cytometry (BD Biosciences, San Jose, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…In all participants, the total EPC count was assessed by using an in vitro assay, as described previously. 8) In brief, mononuclear cells (MNCs) were obtained from peripheral blood samples (100 µL), and the EPCs were identified by flow cytometry (BD Biosciences, San Jose, CA, USA). Their phenotype was determined by immunohistochemistry after staining with 20 µL fluorescein PE-Cy5-conjugated anti-CD45 monoclonal antibody (Dynona, Korea), 20 µL of fluorescein isothiocyanate (FITC)-conjugated anti-CD34 monoclonal antibody (Dynona, Korea) and 10 µL of PE-conjugated anti-vascular endothelial growth factor receptor 2 (VEGFR2) monoclonal antibody (R&D, Minneapolis, MN, USA), and further incubated in a dark room for 1 hour.…”

Section: Methodsmentioning

confidence: 99%

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The Number of Endothelial Progenitor Cells is Decreased in Patients With Non-Dipper Hypertension

Kim

et al. 2012

Korean Circ J

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Background and ObjectivesCirculating endothelial progenitor cells (EPCs) play a key role in the maintenance of endothelial homeostasis and promote vascular repair. A reduced number of EPCs and the functional activity have been associated with several cardiovascular risk factors. However, the relationship between the number of EPCs and circadian rhythm of the blood pressure (BP) remains unclear. The purpose of the present study was to evaluate the relationship between the circadian rhythm of the BP and EPCs in patients with essential hypertension.Subjects and MethodsA total of 45 patients with essential hypertension who were newly identified by outpatient BP measurements, underwent 24-hour ambulatory BP monitoring. Among the 45 patients with essential hypertension, 20 were classified as dippers (12 men and 8 women; mean age 48±14 years) and 25 as non-dippers (14 men and 11 women; mean age 52±18 years). The EPC count was isolated from the peripheral bloodstream and quantified by flow cytometry.ResultsThe baseline clinical characteristics were similar between the dipper and non-dipper hypertensive patients. The circulating EPCs were statistically reduced in the non-dipper patients as compared to the dippers (104±60 vs. 66±47 EPCs per 106 mononuclear cells, p=0.027). The circulating EPC level correlated positively with the circadian changes in the systolic and diastolic BP (r=0.435, p=0.003, and r=0.310, p=0.038, respectively).ConclusionThe present study demonstrated that the EPC count was reduced in the peripheral bloodstream in non-dipper hypertensive patients.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%