Effect of EACA, PAMBA, AMCA and AMBOCA on Fibrinolysis Induced by Streptokinase, Urokinase and Tissue Activator

Abstract

A study is presented in which the inhibiting effects of EACA, PAMBA, AMCA and AMBOCA on fibrinolysis induced by three activators have been compared by a clot lysis method. The order of inhibitory potency was AMBOCA > AMCA > PAMBA > EACA. The activity ratios obtained were found to be influenced by the level of inhibition. At low levels of inhibition the differences between the inhibitory activities are usually more pronounced than at high levels. The degree of inhibition is dependent on the fibrinolytic activator used. The order of sensitivity to the inhibitors was, within the analytical conditions used, found to be: tissue activator > streptokinase > urokinase. The results are discussed in comparison to the different activity ratios reported by others.

Effects of Antibiotics on Fibrinolytic Activity

“…LMOX and CBPC showed no effect on the fibrin lysis time, but it was increased significantly by DMD at 1000 ug/ml and CTX at 3000 acg/ml. EACA prolonged the fibrin clot lysis time at 100 /-cg/ml and AMCHA prolonged it at 30 iig/ml, con firming the report of Westlund et al (9).…”

ε-Aminocaproic Acid (EACA)

“…The ion bonds are strengthened by hydrophobic interactions between middle part of EACA with LBS. The more efficient antifibrinolytic activity of EACA analogues with the higher hydrophobicity of middle part of the structure as p-aminomethylbenzoic acid (PAMBA) and t-4-aminomethylcyclohexanocarboxylic acid (AMCA) confirm the role of these interactions (4,13). It is illustrated by the data presented in tab.…”

“…In the concentration 0.015 mg/ml, it inhibits plasminogen activation by tissue plasminogen activator (t-PA), urokinase plasminogen activator (u-PA), streptokinase (SK) and staphylokinase (SFK) (4) (fig. 1).…”

“…The purpose of this procedure is a removal of plasminogen, plasminogen activators, inhibitors of activators and antiplasmin. TCA is removed by the ether extraction and HClO 4 is precipitated with the use of sodium hydroxide. Antifibrinolytic activity of EACA is determined by the measurement of the time of fibrinolysis using fibrinogen, plasmin and thrombin.…”

Medical interventions for traumatic hyphema

“…Tranexamic acid also binds to fibrin and is believed to act through a similar mechanism. The action of aminobenzoic acid involves inhibition of fibrinolysis, and estrogens decrease antithrombin activity, both of which result in delays of clot resorption (Westlund 1982). In addition to inhibition of fibrinolysis, corticosteroids are also believed to stabilize the blood-ocular barrier and reduce inflammation.…”