Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus

Armengol, Josep; Alaniz, Sandra; Civera, Antonio Vicent; Beltrán, Roberto; Abad-Campos, Paloma; Pérez‐Sierra, A.; García‐Jiménez, J.; Salem, Imen Ben; Souli, María; Boughalleb, N.

doi:10.1016/j.funbio.2010.12.007

Cited by 11 publications

(13 citation statements)

References 21 publications

(42 reference statements)

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“…In our study, there wasn't any clear evidence that a decrease in osmotic potential produced a reduction of mycelial growth as previously observed in other fungal species (Imolehin et al, 1980;Lira-Mendez and Mayek-Perez, 2006;Palmero et al, 2008;Armengol et al, 2011). A reduction of the growth of the mitovirus-infected isolates was observed at -0.6 MPa and -1.8 MPa which can be linked to a decrease in the virulence of the isolates.…”

Section: Discussionsupporting

confidence: 60%

“…A reduction of the growth of the mitovirus-infected isolates was observed at -0.6 MPa and -1.8 MPa which can be linked to a decrease in the virulence of the isolates. Changes in behavior were also observed in isolates with and without viral infection when growing at certain osmotic potentials for Monosporascus cannonballus (Armengol et al, 2011).…”

Section: Discussionmentioning

confidence: 88%

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Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

2012

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Mitoviruses have been found in several forest pathogens (i.e. Cryphonectria parasitica, Gremmeniella abietina), and because they have been shown to reduce the virulence of host fungi there is a growing interest in studying their use as a biocontrol. This study was carried out to test the effect of temperature (5°C, 15°C, 25°C and 35°C), pH (4, 5, 7 and 9) and osmotic potential (-0.6, -1.2, -1.8 and -2.4 MPa) on the mycelial growth of seven G. abietina isolates under controlled laboratory conditions. Four of the isolates hosted mitoviruses and three of them did not. During the experiment, mycelial growth was recorded every week for a period of 8 weeks. Results showed no differences in growth behavior between mitovirus infected and non-infected isolates when placed under different pH modifications. However, the mitovirus-infected isolates presented larger mycelial growth than the mitovirus-free ones when at the fungi's optimal growing temperature of 15°C. When growing at certain osmotic potentials (-0.6 and -1.8 MPa) a reduction in growth of the mitovirus-infected isolates was observed. The results of this experiment suggest that mycelial growth among non-infected isolates and isolates naturally infected by mitovirus vary under different culture conditions, thus providing further insight into the effects of mitovirus on Gremmeniella abietina isolates.Key words: mitoviruses; Scleroderris canker; in vitro; biological control; Gremmeniella abietina; dsRNA. Resumen Efecto de posibles mitovirus en el crecimiento in vitro de aislados de Gremmeniella abietina bajo diferentes condiciones de laboratorioLos mitovirus son virus exclusivamente fúngicos que han sido aislados de algunos patógenos forestales (i.e. Cryphonectria parasitica, Gremmeniella abietina) y ya que pueden reducir la virulencia del hongo existe un creciente interés por su posible papel como agentes de control biológico. Se ha llevado a cabo un estudio para evaluar el efecto de la temperatura (5°C, 15°C, 25°C y 35°C), el pH (4, 5, 7 y 9) y el potencial osmótico (-0.6, -1.2, -1.8, -2.4 MPa) en el crecimiento micelial de siete aislados de G. abietina bajo condiciones controladas de laboratorio. Cuatro de los aislados albergaban mitovirus y tres de ellos no. Durante el experimento, el crecimiento micelial fue registrado semanalmente hasta completar 8 mediciones. Los aislados infectados con mitovirus presentaron mayor crecimiento micelial que los no infectados a la temperatura de crecimiento óptimo del hongo de 15°C. No se observaron efectos de la presencia de mitovirus entre los aislados infectados y los no infectados en los tratamientos de modificación del pH. Cuando se modificaron los potenciales osmóticos se observó una reducción del crecimiento micelial de los aislados infectados con mitovirus en los potenciales osmóticos de -0.6 y -1.8 MPa. Los resultados de este experimento sugieren que la presencia de los mitovirus afecta al crecimiento micelial del hongo bajo distintas condiciones de laboratorio. Este estudio proporciona un conocimiento más prof...

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 88%

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

2012

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“…The highest rates of mycelial growth at 28ºC were agreement with previous reports from Israel (Pivonia et al, 2002), Japan (Uematsu & Sekiyama, 1990), Spain (Armengol et al, 2011) and the USA (Stanghellini et al, 2000), where many isolates had optimal growth between 28 and 32ºC. Growth rates of all M. cannonballus isolates at 38ºC were similar to those obtained by Armengol et al (2011), but differed from that found by Pivonia et al (2002), who showed that the hyphal growth of the M. cannonballus included in this study ceased at 39ºC.…”

Section: Discussionsupporting

confidence: 92%

“…This is in agreement with the observations of Martyn and Miller (1996) and with the results of a study conducted with Spanish and Tunisian isolates of this fungus (Armengol et al, 2011). Univariate and multivariate analyses revealed that this variation was mainly due to differences among isolates and there was no effect of production site and/or area.…”

Section: Discussionsupporting

confidence: 92%

“…Growth rates of all M. cannonballus isolates at 38ºC were similar to those obtained by Armengol et al (2011), but differed from that found by Pivonia et al (2002), who showed that the hyphal growth of the M. cannonballus included in this study ceased at 39ºC. Perithecia production was greater at 28ºC, confirming previous observations (Martyn & Miller, 1996;Waugh et al, 2003;Armengol et al, 2011), which revealed that the temperature range between 25 and 30ºC is the most suitable temperature for perithecia production. Moreover, ascospore production was also higher at 28 o C and we did not observe perithecia at 38°C.…”

Section: Discussionsupporting

confidence: 76%

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Fitness components of Monosporascus cannonballus isolates from northeastern Brazilian melon fields

Correia¹,

Silva²,

Câmara³

et al. 2014

Trop. plant pathol.

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Monosporascus root rot and vine decline caused by Monosporascus cannonballus is one of the most important melon yieldlimiting diseases in northeastern Brazil. This study investigated the fitness components of 57 isolates of M. cannonballus obtained from Brazilian melon fields by evaluating: i) their mycelial growth rate (MGR), and perithecia and ascospore production (PP and AP) on potato dextrose agar (PDA); ii) their sensitivity to the fungicide fluazinam; and iii) their virulence to melon seedlings. All M. cannonballus isolates showed variability in their MGR, PP and AP values. They were sensitive to the fungicide fluazinam, showing some degree of mycelial growth inhibition (MGI), and were pathogenic to melon seedlings, with a mean disease severity index (DSI) of 62.1%. By univariate analysis, the formation of groups of similarity amongst the isolates of M. cannonballus within each variable was not limited by the area of origin of each isolate, given that in most situations, different isolates of the same area were distributed into distinct groups of similarity. A multivariate cluster analysis allowed the separation of the 57 M. cannonballus isolates in 18 groups of similarity. The fitness variability among M. cannonballus isolates found in this study should be considered when possible sources of resistance are evaluated in melon breeding programs.

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Effect of soil matric water potentials on germination of ascospores of Monosporascus cannonballus and colonization of melon roots by zoospores of Olpidium bornovanus

2014

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Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus

Cited by 11 publications

References 21 publications

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

Fitness components of Monosporascus cannonballus isolates from northeastern Brazilian melon fields

Effect of soil matric water potentials on germination of ascospores of Monosporascus cannonballus and colonization of melon roots by zoospores of Olpidium bornovanus

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