Effect of soil matric water potentials on germination of ascospores of Monosporascus cannonballus and colonization of melon roots by zoospores of Olpidium bornovanus

Stanghellini, M. E.; Mohammadi, Mojtaba; Adaskaveg, J. E.

doi:10.1007/s10658-014-0395-8

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…Recent studies have reported that the germination of M. cannonballus ascospores is straightly mediated by Olpidium bornovanus (Sahtiy.) Karling, an obligate root pathogen, zoosporic (Stanghellini and Misaghi, 2011;Stanghellini et al, 2014;Aleandri et al, 2017), and vector of the Melon necrotic spot virus -MNSV, also associated with the MRRVD in melon (Herrera-Vásquez et al, 2010). Correspondingly, it is important to emphasize that melon and watermelon areas, especially those with high incidence of MRRVD and low productivity, are utilized for cultivation of other crops in order to reduce the fungal inoculum and maintain the activity in these fields.…”

Section: Resultsmentioning

confidence: 99%

Soils of Tropical Dry Forest and with Different Crops Presenting Ascospores of Monosporascus cannonballus

Júnior

Senhor

Medeiros

et al. 2018

Not Bot Horti Agrobo

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The vine decline caused by Monosporascus cannonballus is a limiting factor in different crops in several countries. The objective of this study was to quantify the M. cannonballus ascospores in soils covered with tropical dry forest and areas cultivated with pineapple, cotton, coconut, corn, mango, melon, papaya, sorghum and watermelon. Five areas were sampled in tropical dry forest and every crop. The M. cannonballus ascospores were extracted using the flotation method of sucrose. Ascospores of M. cannonballus were detected in all soil samples from Rio Grande do Norte and Ceará states, including tropical dry forest. There were significant differences among the ascospores densities of M. cannonballus, which varied from 0.55 to 2.21 ascospores g-1 soil. The lower densities were found in areas with cotton, coconut, mango, pineapple, and melon within the first and fifth years of cultivation, in addition to uncultivated areas of tropical dry forest. The highest ascospores density was found in papaya areas. Up to date, there is no study to prove that this crop is considered host of this phytopathogen. Cultivated areas with cucurbitaceous with more years of cultivation presented higher densities of M. cannonballus ascospores in soils from Brazilian semiarid. However, there is no direct relationship between M. cannonballus population density in the soil and the susceptibility of the host being cultivated in the soil at the time of sampling.

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Section: Resultsmentioning

confidence: 99%

Soils of Tropical Dry Forest and with Different Crops Presenting Ascospores of Monosporascus cannonballus

Júnior

Senhor

Medeiros

et al. 2018

Not Bot Horti Agrobo

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“…It has been reported that the obligate, host‐specific, root‐infecting zoosporic fungus, Olpidium bornovanus , is directly involved in the induction of germination of ascospores of M. cannonballus in commercial melon fields in Arizona and California (Stanghellini and Misaghi ; Stanghellini et al. ). Similarly, we have recently assessed the presence of O. bornovanus and O. virulentus in the soil where the present trial was carried out, and we have ascertained their ability to colonize the roots of melon (Aleandri et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of Preconditioning Through Mycorrhizal Inoculation on the Control of Melon Root Rot and Vine Decline Caused by Monosporascus cannonballus

Aleandri

Martignoni

Reda

et al. 2015

Journal of Phytopathology

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The use of inoculum of arbuscular mycorrhizal fungi (AMF) in nursery represents a promising field in horticulture because of its known benefits in terms of plant growth and bioprotection. The present work was undertaken to determine the effect of mycorrhizal inoculation with Rhizophagus irregularis in a nursery medium on the containment of melon root rot and vine decline (MRRVD) caused by the soil-borne pathogen Monosporascus cannonballus. The percentage of mycorrhization, biomass and yield following mycorrhizal inoculation were also evaluated. Biocontrol activity was assessed in greenhouse pot experiments upon artificial inoculation of M. cannonballus and in a two-season field experiment under production conditions in an unheated greenhouse with a history of MRRVD. On the basis of the mycorrhization parameters, the interaction appeared to be established within 30 days after inoculation. The total shoot growth in the mycorrhized plants was significantly higher when compared to the control, while the root growth was unaffected. Upon artificial inoculation of M. cannonballus, mycorrhization provided complete protection against the pathogen. Greenhouse experiments under production conditions during spring cropping season showed that pretransplanting inoculation with R. irregularis significantly decreased the severity of the disease. Also, the average fruit weight of mycorrhized plants was significantly higher than the untreated control. Nevertheless, in summer crop, the bioprotection activity of AMF failed. Present results indicate that the use of AMF in a nursery setting can contribute to the prevention of the onset of this problematic soil-borne disease within a sustainable and integrated soil-borne disease management.

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“…Host selection is relatively specific, with species representing only part of the Cucurbitaceae family acting as hosts [10]. The virus can cause necrosis of roots, leaves and fruits, from which the virus is transmitted mainly by the chytrid fungus Olpidiumbornovanus in nature [11][12][13][14]. In addition, MNSV can also be spread through mechanical inoculation [7] and propagation via infected seeds [10,15].…”

Section: Introductionmentioning

confidence: 99%

Detection of melon necrotic spot virus by one-step reverse transcription loop-mediated isothermal amplification assay

Qiao¹,

Dai²,

Liu³

et al. 2020

PLoS ONE

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Melon necrotic spot virus (MNSV) can cause significant economic losses due to decreased quality in cucurbit crops. The current study is the first to use reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of MNSV. A set of four LAMP primers was designed based on the coat protein gene sequence of MNSV, and a RT-LAMP reaction was successfully performed for 1 h at 62˚C. The results of RT-LAMP showed high specificity for MNSV and no cross-reaction with other viruses. Compared to traditional reverse transcription-PCR (RT-PCR), the RT-LAMP assay was 10 3-fold more sensitive in detecting MNSV. Due to its sensitivity, speed and visual assessment, RT-LAMP is appropriate for detecting MNSV in the laboratory.

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Effect of soil matric water potentials on germination of ascospores of Monosporascus cannonballus and colonization of melon roots by zoospores of Olpidium bornovanus

Cited by 7 publications

References 11 publications

Soils of Tropical Dry Forest and with Different Crops Presenting Ascospores of Monosporascus cannonballus

Soils of Tropical Dry Forest and with Different Crops Presenting Ascospores of Monosporascus cannonballus

Effects of Preconditioning Through Mycorrhizal Inoculation on the Control of Melon Root Rot and Vine Decline Caused by Monosporascus cannonballus

Detection of melon necrotic spot virus by one-step reverse transcription loop-mediated isothermal amplification assay

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