Effect of drying treatments on the contents of lutein and zeaxanthin in orange- and yellow-cultivars of marigold flower and its application for lutein ester encapsulation

Kurniawan, Jovine Marcella; Yusuf, Melisa Megawati; Azmi, Sherly Salsabila; Salim, Katarina Purnomo; Prihastyanti, Monika Nur Utami; Indrawati, Renny; Heriyanto, Heriyanto; Shioi, Yuzo; Limantara, Leenawaty; Brotosudarmo, Tatas Hardo Panintingjati

doi:10.1088/1757-899x/509/1/012060

Cited by 17 publications

(15 citation statements)

References 37 publications

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“…Literature data for a Tagetes erecta cultivar harvested on Bali and called Mega Orange show lutein and zeaxanthin range from 8950 to 14550 mg•kg -1 of dry matter. The content we determined was lower as well as that of another cultivar called Mega Gold also from Bali (2560-3730 mg•kg -1 of dry matter) (Kurniawan et al, 2019). The determined xanthophyll content was also expressed on fresh matter for comparison with the published literature data.…”

Section: Resultsmentioning

confidence: 57%

Determination of carotenoids in flowers and food supplements by HPLC-DAD

Pavelková

Krmela

Schulzová

2020

Acta Chimica Slovaca

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Marigold flowers (Tagetes patula and Calendula officinalis) were chosen for analysis because they are the most often used source of lutein and its isomer zeaxanthin for the production of food supplements on the Czech market. Direct extraction and extraction with alkaline hydrolysis were compared to detect free or bound carotenoids. For carotenoid separation, C18 and C30 columns were used. A new method for determination of carotenoid content in food supplements in form of capsules has been developed and validated. All matrices were analysed by high-performance liquid chromatography with diode array detection (HPLC-DAD). It has been found that alkaline hydrolysis is required for both Marigold flowers and food supplements to release lutein from ester bonds to fatty acids. In Calendula officinalis lutein in the concentration of 807—1472 mg·kg−1 of dry matter was detected. Tagetes patula has been identified as a better lutein source with the content of 5906—8677 mg·kg−1 of dry matter. It has been found that the content of lutein and zeaxanthin in commercial food supplements (Lutein Complex Premium and Occutein Brillant) is consistent with the declared quantity. Linearity of the HPLC-DAD method ranged from 0.1—20 μg·mL−1 with the limit of quantification (LOQ) of 1.7 mg·kg−1 for lutein in Marigold flowers and 200 mg·kg−1 in food supplements. Repeatability was 2.3 % for lutein in all tested matrices.

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Section: Resultsmentioning

confidence: 57%

Determination of carotenoids in flowers and food supplements by HPLC-DAD

Pavelková

Krmela

Schulzová

2020

Acta Chimica Slovaca

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“…n-hexane, water, MTBE, and methanol to extract the astaxanthin in organic phase and then the organic phase was dried using a N2 gas. The dried carotenoid extract was dissolved in acteone and at least 20 μL extract was injected to the HPLC according to the method of Kurniawan et al [25] with a slight modification. The total percentage peak area of freeastaxanthin peaks in the SK extract were determined from the HPLC chromatogram detected at 475 nm.…”

Section: Determination Of Carotenoid Content In the Sk Extract And Thmentioning

confidence: 99%

Mie Krill: Incorporation of Indigenous Udang Saih (Sumatran Krill) Extract Containing Astaxanthin and Essential Fatty Acids into an Instant Noodle Product Made from Composite Flour

Gunawan

Kurniawan

Sudarsono

et al. 2021

Indones. J. Nat. Pigm.

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Indonesia is an archipelagic country. One can be found as the indigenous specimen alongside the coastal part of the country is the Sumatran Krill (SK), commonly known as ‘Udang Saih’ which can be found in Padang, West Sumatra. While the local people use it to make traditional condiment (like shrimp paste), the krill itself could be extracted to create more valuable products since it contains various important unsaturated fatty acids such as omega-3, omega-6, omega-7, and omega-9 and the powerful antioxidant carotenoid named astaxanthin. Astaxanthin as the main carotenoid in SK extract offers several health benefits such as anti-inflammatory properties, support immune system modulation, as well as maintaining brain, cardiovascular, and eye health. In this study, we aim to incorporate the SK extract into an instant noodle product. In addition, we used the composite flours consisted of high-protein wheat flour (WF) and yellow sweet potato flour (YSPF) which are rich in β-Carotene as a vitamin A precursor, to further enhance its eye health property. The instant noodle prototype (M2) was made from a mixture of YSPF (40 g), WF (40 g), super-concentrated astaxanthin in sunflower oil (8 mL), and super-concentrated astaxanthin (2.4 mL) had the highest score of sensory attributes. This instant noodle prototype was claimed to contain astaxanthin, essential fatty acids, and provitamin A carotenoid as well as low in gluten content. Therefore, this instant noodle prototype is believed to increase the health benefits and can be well accepted in society.

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“…Twenty μL of each sample was used for the determination of the carotenoid content, following the method of Kurniawan et al [29], using separation with a Shimadzu highperformance liquid chromatograph (HPLC) on a YMC carotenoid C- International Journal of Food Science (α-car), and β-carotene (β-car), were obtained by using the standard pigment manufactured by NATChrom (Malang, Indonesia). The linear equations were used to determine the concentrations of the lutein (y = 305:8x -0:6335, R 2 = 0:9999), α-car (y = 232:04x + 47:906, R 2 = 0:995), and β-car (y = 206:57x + 74:953, R 2 = 0:9982) (where y is the peak area and x is the concentration of the determined pigment) in μg/mL, at their maximum absorption wavelengths (λ max ) of 445, 445, and 450 nm, respectively.…”

Section: Carotenoid Extraction and Hplc Analysismentioning

confidence: 99%

Evaluating Provitamin A Carotenoids and Polar Metabolite Compositions during the Ripening Stages of the Agung Semeru Banana (Musa paradisiaca L. AAB)

Chandra

Siswanti

Prihastyanti

et al. 2020

International Journal of Food Science

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Banana cultivars that are rich in provitamin A carotenoids and other nutrients may offer a potential food source to help alleviate vitamin A deficiencies, particularly in developing countries. The local plantain type banana, Agung Semeru (Musa paradisiaca L.), was investigated, in order to analyse the changes in the compositions of the provitamin A carotenoids and metabolite compounds, including the amino acids, organic acids, and sugars, during the ripening stage as this banana is widely processed for food products in either the unripe, ripe, or overripe stages. The bananas that had reached the desired ripening stages were subjected to high-performance liquid chromatography (HPLC) analysis, and the results indicated that the total provitamin A carotenoid concentrations ranged between 4748.83 μg/100 g dry weight (dw) and 7330.40 μg/100 g dw, with the highest level of vitamin A activity at 457.33±5.18 μg retinol activity equivalents (RAE)/100 g dw. Compared to the Cavendish variety, which is consumed worldwide, the Agung Semeru banana had vitamin A activity that was 40 to 90 times higher, dependent on the stage of ripening. The breakdown of the starch during the ripening stages resulted in an increase of its sugar compounds, such as sucrose, fructose, and glucose, as well as its dominant organic acids, such as malic acid, oxalic acid, and citric acid, which were observed using gas chromatography-mass spectrometry (GC-MS) during the ripening stages. The findings of this study show that the Agung Semeru banana is a promising fruit that could be widely produced as a nutritional and energy food resource, due to its high levels of vitamin A activity and sugars.

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Effect of drying treatments on the contents of lutein and zeaxanthin in orange- and yellow-cultivars of marigold flower and its application for lutein ester encapsulation

Cited by 17 publications

References 37 publications

Determination of carotenoids in flowers and food supplements by HPLC-DAD

Determination of carotenoids in flowers and food supplements by HPLC-DAD

Mie Krill: Incorporation of Indigenous Udang Saih (Sumatran Krill) Extract Containing Astaxanthin and Essential Fatty Acids into an Instant Noodle Product Made from Composite Flour

Evaluating Provitamin A Carotenoids and Polar Metabolite Compositions during the Ripening Stages of the Agung Semeru Banana (Musa paradisiaca L. AAB)

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