Effect of Drought on Seed Germination and Early Seedling of Tomato Genotypes using Polyethylene Glycol 6000

Esan, Vincent Ishola; Ayanbamiji, Taiwo Ayanniyin; Adeyemo, J; Oluwafemi, Sunday

doi:10.18483/ijsci.1533

Cited by 6 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Drought that occurs causes impacts and problems for the world of agriculture, especially on the rate of productivity related to meeting the water needs of a plant (Sujinah and Jamil, 2016) (Venkatesh et al, 2018). Tomato (Solanum lycopersicum) is one of the horticultural crops whose productivity is threatened during the dry season (Esan et al, 2018). Tomato has various benefits and is in great demand by most people (Zhou et al, 2020), especially residents in Indonesia.…”

Section: Introductionmentioning

confidence: 99%

Drought tolerance selection of several tomato varieties by using polyethylene glycol

Avivi,

Sitompul,

Siswoyo

et al. 2023

ipas

View full text Add to dashboard Cite

A prolonged dry season can cause drought stress and have an unfavorable impact on the growth of tomato plants, resulting in crop failure. Therefore, research is needed on the resistance of tomato varieties to drought stress. The recommended technique for drought resistance screening in tomatoes is in vitro cultivation using Polyethylene Glycol (PEG). The research aimed to obtain new varieties that could be used as promising lines for varieties tolerant to drought stress. The research method used included using ingredients in the form of 5 varieties of tomatoes (Ratna, Intan, Chung, Palupi, and Amelia) and 5 concentrations of PEG chemicals (0%, 5%, 10%, 15%, and 20%). Each seed of each tomato variety that had been sterilized using hypochlorite was germinated on MS0 media, then the germinated seeds with a size of ± 0.3 cm were transferred to MS0 + PEG treatment medium with each treatment concentration. The seeds that had been planted on the PEG-treated medium were then observed for growth for ± 4 weeks. The research data were analyzed using analysis of variance (ANOVA) and followed with DMRT at the 5% significance level. Based on the results, Palupi variety is the most tolerant variety, and Ratna variety is the most intolerant variety to drought stress. The higher the concentration of PEG used, the more it inhibits plant height, root length, and fresh weight of the roots.

show abstract

Section: Introductionmentioning

confidence: 99%

Drought tolerance selection of several tomato varieties by using polyethylene glycol

Avivi,

Sitompul,

Siswoyo

et al. 2023

ipas

View full text Add to dashboard Cite

show abstract

“…PEG 6000 is a natural polymer, which is nontoxic and nonionic. PEG 6000 induces osmotic stress in plants by reducing water potential as it is observed during the shortage of rainfall [15,16].…”

Section: Introductionmentioning

confidence: 99%

Root System Architecture and Physiological Characteristics of Soybean (Glycine max L.) Seedlings in Response to PEG6000-Simulated Drought Stress

Esan

Obisesan

Ogunbode

2023

International Journal of Agronomy

View full text Add to dashboard Cite

The production of soybean is restricted in sub-Saharan Africa by several stress conditions, including drought because its production is exclusively rain-fed. Identifying drought resistant varieties is of paramount importance. Thus, the objectives of this work were to (i) evaluate the effect of polyethylene glycol 6000 (PEG6000) on soybean at the seedling stage, (ii) determine the root system architecture and physiological characters to water deficit stress, and (iii) establish the correlation among the quantitative variables responsible for drought tolerance in soybean varieties. Twenty soybean accessions (G1 to G20) were subjected to 10% PEG6000 concentration at seedling stages under a controlled environment using a randomized complete block design with 3 replicates. Vegetative growth data were collected. Highly significant differences P < 0.0001 of proline, carotenoid, chlorophyll a, and chlorophyll b contents were recorded among the 20 accessions in response to PEG application. G16 and G19 had the highest carotenoid, highest chlorophyll a, and chlorophyll b. The highest dry weight was observed in G16 and G10, while the number of leaves was recorded in G19 and G17. G4, G9, G10, and G13 demonstrated the highest dry weight. The PEG-simulated drought stress reduced the average root diameters and the number of lateral roots of all 20 accession plants. G1, G3, G4, G8, G9, and G15 had the longest roots than the control plants as a mechanism to withstand drought stress by seeking water in the deep. Number of leaves was significantly and positively correlated with shoot dry weight, root dry weight, and root diameter but was significantly and negatively correlated with canopy wilting. Proline content was significantly and positively correlated with carotenoid, chlorophyll content, chlorophyll a, and chlorophyll b. G10, G19, G9, G6, G16, G17, G20, G16, and G18 are the tolerant cultivars to drought stress on the basis of growth, physiological, and root system architecture.

show abstract

“…PEG molecules are too large to be absorbed by plant roots, so the increased PEG concentration in the surrounding environment causes the water to not be absorbed by the stem cells and thus the plant is exposed to water stress (Mohammadkhani et al, 2008;Hamayun et al, 2010). The drought stress created by PEG can also be used for in vitro screening tests, so a large number of genetic resources can be accurately screened with less effort (Kulkarni and Deshpande, 2007;Basha et al, 2015;Esan et al, 2018;Özkaynak and Şimşek, 2018). It has been reported that PEG concentrations ranging from 4% to 12.5% are suitable for screening of tomato genotypes against drought tolerance during germination and seedling stage (Ghebremariam et al, 2013;Jokanovic and Zdravkovic, 2015).…”

Section: Introductionmentioning

confidence: 99%

Domates Genotiplerinin Kuraklık Stresine Tolerans Açısından Taranmasında In Vitro ve In Vivo Testlerin Etkinliği

Çoban

Altunlu

Gül

2020

Ege Üniversitesi Ziraat Fakültesi Dergisi

View full text Add to dashboard Cite

Objective: The aim of this study was to investigate possibilities of fast screening of local tomato genetic material against drought. Material and Methods: In this study including in vitro and in vivo tests, seeds of 92 tomato genotypes were used and drought stress was induced by polyethylene glycol 6000 (PEG) at 4%. Firstly, seed germination test was made; and 5 genotypes with higher and 5 genotypes with lower performances were selected according to the evaluation made at 14 days. These genotypes were tested by water culture. Full drought dose was Ψs= -1.0 MPa and 48 hours after the full dose application, morphological and physiological properties of the plants were determined. The genotypes tested were classified by weighted ranking method, based on the changes in the PEG treatment compared to the control. Results:The correlation coefficient (r: 0.824) for the relationship between the variation (%) of in vitro vigour index and the total score of weighted ranking in water culture was significant. As a result of this study; 97:TR70707, 68:TR69163 and 60: TR68515 in the genetic pool tested, were determined as the most tolerant genotypes against drought. Conclusion:It was concluded that in vitro seed germination test can be used for prescreening of large numbers of genotypes in response to drought stress. ÖZAmaç: Bu çalışma yerel domates genetik materyalinin kuraklığa karşı hızlı bir şekilde tarama olanaklarını araştırmak amacıyla yürütülmüştür. Materyal ve Metot:In vitro ve in vivo testleri kapsayan çalışmada, 92 domates genotipinin tohumları kullanılmış ve kuraklık stresi %4'lük polietilen glikol (PEG) 6000 ile yaratılmıştır. İlk aşamada, tohum çimlendirme testi yapılmış; 14. günde yapılan değerlendirmeye göre daha yüksek performans gösteren 5 genotip ve daha düşük performans gösteren 5 genotip in vivo test için seçilmiştir. Bu genotipler su kültürü tekniği kullanılarak test edilmiştir. Tam kuraklık dozu olarak Ψ s = -1.0 MPa kullanılmış ve tam doz uygulamasından 48 saat sonra bitkilerin morfolojik ve fizyolojik özellikleri belirlenmiştir. PEG uygulamasında kontrole kıyasla meydana gelen değişim değerleri dikkate alınarak, genotipler "tartılı derecelendirme" yöntemine göre sınıflandırılmıştır. Bulgular: In vitro vigor indeksindeki % değişim değerleri ile in vivo tartılı derecelendirme toplam puanları arasındaki korelasyon katsayısı (r: 0.824) önemli bulunmuştur. Sonuç olarak, test edilen genetik havuz içerisinde 97:TR70707, 68:TR69163 ve 60: TR68515 numaralı genotiplerin kurağa toleransının yüksek olduğu saptanmıştır. Sonuç: In vitro tohum çimlendirme testinin fazla sayıda genotipin kurağa tolerans bakımından ön değerlendirmesine uygun olduğu düşünülmektedir.

show abstract

Effect of Drought on Seed Germination and Early Seedling of Tomato Genotypes using Polyethylene Glycol 6000

Cited by 6 publications

References 8 publications

Drought tolerance selection of several tomato varieties by using polyethylene glycol

Drought tolerance selection of several tomato varieties by using polyethylene glycol

Root System Architecture and Physiological Characteristics of Soybean (Glycine max L.) Seedlings in Response to PEG6000-Simulated Drought Stress

Domates Genotiplerinin Kuraklık Stresine Tolerans Açısından Taranmasında In Vitro ve In Vivo Testlerin Etkinliği

Contact Info

Product

Resources

About