Domates Genotiplerinin Kuraklık Stresine Tolerans Açısından Taranmasında In Vitro ve In Vivo Testlerin Etkinliği

Çoban, Gökçe Aydöner; Altunlu, Hakan; Gül, Ayşe

doi:10.20289/zfdergi.835798

Cited by 2 publications

(1 citation statement)

References 21 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even the outliers displayed a much lower LWP when submitted to drought stress. Despite the wide range of methodologies proposed to induce drought stress in tomatoes [ 13 , 27 , 52 , 53 ], multiple studies, from the pioneering work of Wudiri [ 25 ] to the recent experiments of Cui [ 26 ], suggest that the flowering and fruit-setting stage is when tomato plants are the most sensitive to drought. Therefore, promoting severe water deprivation (soil almost reaches wilting point) during this stage, as we did in our study, proved to be an accurate method of screening and selecting plants exhibiting drought tolerance.…”

Section: Discussionmentioning

confidence: 99%

Uncovering tomato candidate genes associated with drought tolerance using Solanum pennellii introgression lines

et al. 2023

View full text Add to dashboard Cite

Tomato plants are sensitive to drought stress throughout their growth cycle. To be considered drought-tolerant, a cultivar should display tolerance at all developmental stages. This study aimed to evaluate whether Solanum pennellii introgression lines (ILs) previously selected as drought-tolerant during germination/seedling growth maintained this tolerance in the vegetative/reproductive stage. We then investigated these ILs to uncover candidate genes. The plants were subjected to two different environmental conditions: well-watered and drought-stressed (water withheld for ≤ 20 d after flowering). Phenotyping for morphological, physiological, fruit quality, and yield-related traits was performed, and the data was analyzed using a mixed-model approach. Using a multi-trait index that relies on factor analysis and genotype-ideotype distance (FAI-BLUP index), the genotypes were ordered based on how far they were from the drought-tolerant ideotype. Afterward, the tomato IL population map furnished by the SOL Genomics Network was utilized to identify introgressed segments of significance for the identification of candidate genes. Significant genotypic differences were found in the yield, water content, mean weight, length, and width of the fruit, the percentage of fruits displaying blossom-end rot, and titratable acidity. The drought-tolerance ideotype was built considering the maximum values for the fruit water content, number of fruits, mean fruit weight, and yield, minimum values for blossom-end rot, and mean values for titratable acidity. IL 1-4-18, IL 7-4-1, IL 7–1, IL 7-5-5, and IL 1–2 were ranked above M-82 and therefore considered drought-tolerant during the vegetative/reproductive stage. IL 1-4-18 and IL1-2 sustained drought tolerance displayed during germination/seedling growth into the vegetative/reproductive stage. The following candidate genes associated with drought tolerance were identified: AHG2, At1g55840, PRXIIF, SAP5, REF4-RELATED 1, PRXQ, CFS1, LCD, CCD1, and SCS. Because they are already associated with genetic markers, they can be transferred to elite tomato cultivars through marker-assisted technology after validation.

show abstract

Section: Discussionmentioning

confidence: 99%

Uncovering tomato candidate genes associated with drought tolerance using Solanum pennellii introgression lines

et al. 2023

View full text Add to dashboard Cite

show abstract

<i>In Vitro</i> Germination and Early Vegetative Growth of Five Tomato (<i>Solanum lycopersicum</i> L.) Varieties under Water Stress Conditions

Sané¹,

Diallo²,

Kane³

et al. 2021

AJPS

View full text Add to dashboard Cite

Water is the main limiting factor in the cultivation of tomato (Solanum lycopersicum L.) in Senegal. Thus, the selection of varieties tolerant to water stress would be an alternative solution for their production. In vitro germination, growth, total chlorophyll and proline levels were studied in five varieties of tomato subjected to increasing osmotic pressures (0, 5, 10 and 15 kPa) thanks to the PEG-8000 incorporated in an MS/2 medium for 30 days. A strong sensitivity to water stress for in vitro seed germination in the Rodeo variety (41%) is recorded at 5 kPa and maintained at 15 kPa (20.83%) while it was only noticed at 15 kPa in the other tomato varieties. The Xewel and Lady Nema varieties obtained the smallest reductions in the number of leaves of vitroplants, with 30.79% and 27.97% at 15 kPa, respectively, and the Rodeo variety recorded a reduction of 35.97%. From 5 kPa, the varieties record reductions in the number of secondary roots of more than 15%. The effect of osmotic pressures on decreasing the taproot height and length is not significant. The Xewel variety had the highest average fresh (0.483 g) and dry (0.082 g) weights of the aerial part at 15 kPa and the Rodeo variety had the lowest ones (0.308 g and 0.0501 g). The Lady Nema variety had the highest average fresh (0.171 g) and dry (0.039 g) root weights and the Rodeo variety had the lowest ones (0.086 and 0.020 g). The vitroplants of Rodeo variety recorded the highest decreases in total chlorophyll contents at all osmotic pressures and the lowest increase in proline content (53.37%) at 15 kPa. A contrario, the Xewel variety recorded the greatest increase in proline content (116.

show abstract

Domates Genotiplerinin Kuraklık Stresine Tolerans Açısından Taranmasında In Vitro ve In Vivo Testlerin Etkinliği

Cited by 2 publications

References 21 publications

Uncovering tomato candidate genes associated with drought tolerance using Solanum pennellii introgression lines

Uncovering tomato candidate genes associated with drought tolerance using Solanum pennellii introgression lines

<i>In Vitro</i> Germination and Early Vegetative Growth of Five Tomato (<i>Solanum lycopersicum</i> L.) Varieties under Water Stress Conditions

Contact Info

Product

Resources

About

Domates Genotiplerinin Kuraklık Stresine Tolerans Açısından Taranmasında In Vitro ve In Vivo Testlerin Etkinliği

Cited by 2 publications

References 21 publications

Uncovering tomato candidate genes associated with drought tolerance using Solanum pennellii introgression lines

Uncovering tomato candidate genes associated with drought tolerance using Solanum pennellii introgression lines

&lt;i&gt;In Vitro&lt;/i&gt; Germination and Early Vegetative Growth of Five Tomato (&lt;i&gt;Solanum lycopersicum&lt;/i&gt; L.) Varieties under Water Stress Conditions

Contact Info

Product

Resources

About

<i>In Vitro</i> Germination and Early Vegetative Growth of Five Tomato (<i>Solanum lycopersicum</i> L.) Varieties under Water Stress Conditions