Effect of downy mildew disease on photosynthesis and chlorophyll fluorescence in Plantago ovata Forsk

Mandal, Kunal; Saravanan, R.; Maiti, Satyabrata; Kothari, I. L.

doi:10.1007/bf03356305

Cited by 50 publications

(36 citation statements)

References 16 publications

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“…Chou et al (2000) showed a similar decrease in chlorophyll content during infection of Arabidopsis thaliana leaves with Albugo candida (white blister rust). The decrease in chlorophyll content evoked by pathogenic fungi was also observed by other authors (Rasmussen and Sheffer 1988;Moriondo et al 2005;Mandal et al 2009a;Zhao et al 2011). Table 4 Changes in the content ([mg g -1 (DW)]) of chlorophyll a (Chla), chlorophyll b (Chlb), total chlorophyll (Chla+b) and carotenoids (Crts) observed 7 and 14 days after the inoculation (I) with L. maculans spores in the leaves of studied cultivars Apart from ROS, other significant predictors of hostpathogen interaction are pathogenesis related (PR) proteins (Scherer et al 2005).…”

Section: Discussionsupporting

confidence: 78%

Carbohydrate, phenolic and antioxidant level in relation to chlorophyll a content in oilseed winter rape (Brassica napus L.) inoculated with Leptosphaeria maculans

Hura

Dziurka

et al. 2015

Eur J Plant Pathol

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The relationships between the level of chlorophyll a, and the content of soluble carbohydrates, phenolics and low molecular antioxidants in the leaves of three oilseed winter rape varieties with different resistance to Leptosphaeria maculans were determined. During pathogenesis, an increase in the content of chlorophyll a in the resistant and medium-sensitive rape varieties was observed. In these varieties, the level of chlorophyll a significantly correlated with the content of primary metabolites in the form of soluble sugars, as well as with the level of free and cell wall-bound phenolics, and lowmolecular antioxidants. In contrast, a decrease in soluble carbohydrates, observed during the pathogenesis in the susceptible variety, was accompanied by lower level of chlorophyll a and with high activity of reactive oxygen species. Significant correlations were also confirmed for the cell wall-bound phenolics and water content in the leaves of the resistant and medium-sensitive variety.

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Section: Discussionsupporting

confidence: 78%

Carbohydrate, phenolic and antioxidant level in relation to chlorophyll a content in oilseed winter rape (Brassica napus L.) inoculated with Leptosphaeria maculans

Hura

Dziurka

et al. 2015

Eur J Plant Pathol

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“…Even though the decrease in F v /F m values due to the pathogen attack is now well documented [32,33], only few studies developed approaches for the quantification of the diseased area on leaves. Most of the studies using the F v /F m parameter in plant pathology are based on the mean F v /F m value over the whole image [23,33,35-37,47]. The mean F v /F m value may qualitatively discriminate between healthy and diseased leaves, but does not quantify the amount of diseased tissues [23,33,35-37,47].…”

Section: Discussionmentioning

confidence: 99%

“…Most of the studies using the F v /F m parameter in plant pathology are based on the mean F v /F m value over the whole image [23,33,35-37,47]. The mean F v /F m value may qualitatively discriminate between healthy and diseased leaves, but does not quantify the amount of diseased tissues [23,33,35-37,47]. Only few studies attempted to analyze the pixel-wise F v /F m -distribution to discriminate between healthy and diseased organs using a threshold of F v /F m .…”

Section: Discussionmentioning

confidence: 99%

High throughput quantitative phenotyping of plant resistance using chlorophyll fluorescence image analysis

et al. 2013

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BackgroundIn order to select for quantitative plant resistance to pathogens, high throughput approaches that can precisely quantify disease severity are needed. Automation and use of calibrated image analysis should provide more accurate, objective and faster analyses than visual assessments. In contrast to conventional visible imaging, chlorophyll fluorescence imaging is not sensitive to environmental light variations and provides single-channel images prone to a segmentation analysis by simple thresholding approaches. Among the various parameters used in chlorophyll fluorescence imaging, the maximum quantum yield of photosystem II photochemistry (Fv/Fm) is well adapted to phenotyping disease severity. Fv/Fm is an indicator of plant stress that displays a robust contrast between infected and healthy tissues. In the present paper, we aimed at the segmentation of Fv/Fm images to quantify disease severity.ResultsBased on the Fv/Fm values of each pixel of the image, a thresholding approach was developed to delimit diseased areas. A first step consisted in setting up thresholds to reproduce visual observations by trained raters of symptoms caused by Xanthomonas fuscans subsp. fuscans (Xff) CFBP4834-R on Phaseolus vulgaris cv. Flavert. In order to develop a thresholding approach valuable on any cultivars or species, a second step was based on modeling pixel-wise Fv/Fm-distributions as mixtures of Gaussian distributions. Such a modeling may discriminate various stages of the symptom development but over-weights artifacts that can occur on mock-inoculated samples. Therefore, we developed a thresholding approach based on the probability of misclassification of a healthy pixel. Then, a clustering step is performed on the diseased areas to discriminate between various stages of alteration of plant tissues. Notably, the use of chlorophyll fluorescence imaging could detect pre-symptomatic area. The interest of this image analysis procedure for assessing the levels of quantitative resistance is illustrated with the quantitation of disease severity on five commercial varieties of bean inoculated with Xff CFBP4834-R.ConclusionsIn this paper, we describe an image analysis procedure for quantifying the leaf area impacted by the pathogen. In a perspective of high throughput phenotyping, the procedure was automated with the software R downloadable at http://www.r-project.org/. The R script is available at http://lisa.univ-angers.fr/PHENOTIC/telechargements.html.

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“…In addition, the source-sink relationship may change due to the deprivation of carbohydrates by the pathogen and an increased nutrient remobilisation in infected leaves may occur in parallel with decreased photosynthetic activity (Prokopová et al, 2010a;Rojas et al, 2014). In Plantago leaves, downy mildew infection may lead to lower contents of soluble sugars and higher starch contents (Mandal et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The latter indicates the degradation of chlorophyll in impacted cells of the infected tissues (Mandal et al, 2009). However, severity of symptoms can be highly variable.…”

Section: Introductionmentioning

confidence: 99%

ФPSII and NPQ to evaluate Bremia lactucae-infection in susceptible and resistant lettuce cultivars

Brabandt

Bauriegel

Gärber

et al. 2014

Scientia Horticulturae

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Effect of downy mildew disease on photosynthesis and chlorophyll fluorescence in Plantago ovata Forsk

Cited by 50 publications

References 16 publications

Carbohydrate, phenolic and antioxidant level in relation to chlorophyll a content in oilseed winter rape (Brassica napus L.) inoculated with Leptosphaeria maculans

Carbohydrate, phenolic and antioxidant level in relation to chlorophyll a content in oilseed winter rape (Brassica napus L.) inoculated with Leptosphaeria maculans

High throughput quantitative phenotyping of plant resistance using chlorophyll fluorescence image analysis

ФPSII and NPQ to evaluate Bremia lactucae-infection in susceptible and resistant lettuce cultivars

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