Carbohydrate, phenolic and antioxidant level in relation to chlorophyll a content in oilseed winter rape (Brassica napus L.) inoculated with Leptosphaeria maculans

Hura, Katarzyna; Hura, Tomasz; Dziurka, Kinga; Dziurka, Michał

doi:10.1007/s10658-015-0680-1

Cited by 10 publications

(7 citation statements)

References 59 publications

(57 reference statements)

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“…Chlorophyll content decreased in tolerant and susceptible B. napus and B. rapa cultivars to A. brassicicola infection with much stronger degradation in susceptible cultivars [49]. Lowered chl a level accompanied by an extensive production of ROS has been also described in susceptible B. napus infected with Leptosphaeria maculans, which induces yellowish necrotic spots [50]. The appearance of spreading chlorosis followed by a decrease in chlorophyll level is also characteristic for other pathosystems of brassicas with pathogenic fungi, such as Sclerotinia sclerotiorum or the oomycete Albugo candida [22].…”

Section: Day Length-dependent Negative Regulation Of Photosynthesismentioning

confidence: 87%

“…In tolerant and susceptible B. napus and B. rapa cultivars, a decrease in the carotenoid levels in response to A. brassicicola has been noted [49]. However, an increase in carotenoid content has been described in resistant B. napus cultivars infected with L. maculans, while in susceptible oilseed cultivars the carotenoid levels decreased [50]. Maintaining the content of carotenoids in infected B. juncea leaves (regardless of daylength regimes), probably, to some extent, balanced the degradation of chlorophylls at the investigated time points (Table S1).…”

Section: Day Length-dependent Negative Regulation Of Photosynthesismentioning

confidence: 99%

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The Effect of Photoperiod on Necrosis Development, Photosynthetic Efficiency and ‘Green Islands’ Formation in Brassica juncea Infected with Alternaria brassicicola

Macioszek

Sobczak

Skoczowski

et al. 2021

IJMS

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The main goal of growing plants under various photoperiods is to optimize photosynthesis for using the effect of day length that often acts on plants in combination with biotic and/or abiotic stresses. In this study, Brassica juncea plants were grown under four different day-length regimes, namely., 8 h day/16 h night, 12 h day/12 h night, 16 h day/8 h night, and continuous light, and were infected with a necrotrophic fungus Alternaria brassicicola. The development of necroses on B. juncea leaves was strongly influenced by leaf position and day length. The largest necroses were formed on plants grown under a 16 h day/8 h night photoperiod at 72 h post-inoculation (hpi). The implemented day-length regimes had a great impact on leaf morphology in response to A. brassicicola infection. They also influenced the chlorophyll and carotenoid contents and photosynthesis efficiency. Both the 1st (the oldest) and 3rd infected leaves showed significantly higher minimal fluorescence (F0) compared to the control leaves. Significantly lower values of other investigated chlorophyll a fluorescence parameters, e.g., maximum quantum yield of photosystem II (Fv/Fm) and non-photochemical quenching (NPQ), were observed in both infected leaves compared to the control, especially at 72 hpi. The oldest infected leaf, of approximately 30% of the B. juncea plants, grown under long-day and continuous light conditions showed a ‘green island’ phenotype in the form of a green ring surrounding an area of necrosis at 48 hpi. This phenomenon was also reflected in changes in the chloroplast’s ultrastructure and accelerated senescence (yellowing) in the form of expanding chlorosis. Further research should investigate the mechanism and physiological aspects of ‘green islands’ formation in this pathosystem.

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Section: Day Length-dependent Negative Regulation Of Photosynthesismentioning

confidence: 87%

Section: Day Length-dependent Negative Regulation Of Photosynthesismentioning

confidence: 99%

The Effect of Photoperiod on Necrosis Development, Photosynthetic Efficiency and ‘Green Islands’ Formation in Brassica juncea Infected with Alternaria brassicicola

Macioszek

Sobczak

Skoczowski

et al. 2021

IJMS

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“…The following parameters were measured in the leaves of rape plants. Chlorophyll content was measured by ethanol-acetone extraction [30]. Malondialdehyde (MDA) content was measured by two-component spectrophotometry [31].…”

Section: Measurement Of Physiological Parameters and Biomassmentioning

confidence: 99%

Effect of Molybdenum on Plant Physiology and Cadmium Uptake and Translocation in Rape (Brassica napus L.) under Different Levels of Cadmium Stress

Han

Wang

Wan

et al. 2020

IJERPH

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This study investigated the beneficial effect of molybdenum (Mo) application on rape plants (Brassica napus L.) grown in a soil polluted by cadmium (Cd). A pot experiment was conducted to determine how different concentrations of exogenous Mo (0, 50, 100, and 200 mg/kg) affect plant physiology, biomass, photosynthesis, cation uptake, and Cd translocation and enrichment in rape plants under Cd stress (0.5 and 6.0 mg/kg). Under single Cd treatment, plant physiological and biochemical parameters, biomass parameters, leaf chlorophyll fluorescence parameters, and macroelement uptake of rape plants decreased, while their malonaldehyde content, proline content, non-photochemical quenching coefficient, and Cd uptake significantly increased, compared to those of the control group (p-values < 0.05). High-Cd treatment resulted in much larger changes in these parameters than low-Cd treatment. Following Mo application, the accumulation of malondialdehyde and proline decreased in the leaves of Cd-stressed plants; reversely, the contents of soluble protein, soluble sugar, and chlorophyll, and the activities of superoxide dismutase and glutathione peroxidase, all increased compared to those of single Cd treatment (p-values < 0.05). Exogenous Mo application promoted shoot and root growth of Cd-stressed plants in terms of their length, fresh weight, and dry weight. The negative effect of Cd stress on leaf chlorophyll fluorescence was substantially mitigated by applying Mo. Exogenous Mo also improved the uptake of inorganic cations, especially potassium (K+), in Cd-stressed plants. After Mo application, Cd uptake and accumulation were inhibited and Cd tolerance was enhanced, but Cd translocation was less affected in Cd-stressed plants. The mitigation effect of Mo on Cd stress in rape was achieved through the immobilization of soil Cd to reduce plant uptake, and improvement of plant physiological properties to enhance Cd tolerance. In conclusion, exogenous Mo can effectively reduce Cd toxicity to rape and the optimal Mo concentration was 100 mg/kg under the experimental conditions.

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“…The total content of SPh was determined using FolinCiocalteu reagent (Singleton & Rossi 1965;Fornalé et al 2014;Hura et al 2015;Jesus et al 2015). The absorbance was measured at 760 nm.…”

Section: Soluble Phenolics (Sph) and Pal Activitymentioning

confidence: 99%

Effects of low-temperature hardening on the biochemical response of winter oilseed rape seedlings inoculated with the spores of Leptosphaeria maculans

Hura

Hura²,

Rapacz

et al. 2015

Biologia

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The aim of the study was to assess the effects of low-temperature hardening (2 • C) on the biochemical compounds and processes that can increase resistance of winter rape to inoculation with Leptosphaeria maculans spores. The study involved an evaluation of the entire pool of phenolic compounds, L-phenylalanine ammonia lyase (PAL) activity, excitation intensity for blue and green fluorescence, catalase (CAT) activity, respiration intensity and heat emission from leaf tissues. All the measurements were performed 24 and 72 hours after the inoculation. Low-temperature hardening, which preceded the inoculation of rape seedlings with spores of L. maculans, caused a significant increase in CAT activity and the level of phenolic compounds. The observed changes in PAL activity reflected the changes in phenolics content. The hardened plants showed a significantly higher intensity of blue fluorescence excitation at 24 and 72 hours after the inoculation, as compared to the non-hardened seedlings. Increased content of phenolic compounds and PAL and catalase activity triggered by the temperature of 2 • C and maintained for 24 hours after the inoculation, may confirm the stimulating effect of the hardening temperature. Intensified emission of blue fluorescence indicating saturation of a cell wall with phenolic compounds makes the cell wall structure less stretchy, more tight and leakproof, and thereby hinders fungal growth through plant tissue.

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Carbohydrate, phenolic and antioxidant level in relation to chlorophyll a content in oilseed winter rape (Brassica napus L.) inoculated with Leptosphaeria maculans

Cited by 10 publications

References 59 publications

The Effect of Photoperiod on Necrosis Development, Photosynthetic Efficiency and ‘Green Islands’ Formation in Brassica juncea Infected with Alternaria brassicicola

The Effect of Photoperiod on Necrosis Development, Photosynthetic Efficiency and ‘Green Islands’ Formation in Brassica juncea Infected with Alternaria brassicicola

Effect of Molybdenum on Plant Physiology and Cadmium Uptake and Translocation in Rape (Brassica napus L.) under Different Levels of Cadmium Stress

Effects of low-temperature hardening on the biochemical response of winter oilseed rape seedlings inoculated with the spores of Leptosphaeria maculans

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