Effect of Donor Embryo Stage on Development of Nuclear Transplants in Cattle

Aono, Fumihito; Kono, Toru; Sotomaru, Yusuke; Takahashi, Tomoko; Ogihara, Isao; Sekizawa, Fumio; Arai, Touru; Nakahara, Tsutomu

doi:10.1262/jrd.40.5_j35

Cited by 3 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The utilization of Ca 2+ -free solution for cell disaggregation has also been reported for the purpose of weakening intercellular adhesion [ 95 , 96 , 97 ]. In the embryo nuclear transfer procedure, trypsin solution is used to prepare donor cells from cleavage-stage embryos [ 98 , 99 ]. However, the differences in the effectiveness of these media for blastomere separation and the subsequent effects on embryo development have not yet been clarified.…”

Section: Establishment Of Efficient Production System For Monozygoticmentioning

confidence: 99%

The contribution of efficient production of monozygotic twins to beef cattle breeding

Hashiyada

2017

Journal of Reproduction and Development

View full text Add to dashboard Cite

Production of sires with high breeding potential is indispensable for prompt and reliable breeding using their semen in the cattle industry. Currently, in Japan, we aim to further the production of Japanese black sires via a new breeding system that uses genetically homologous monozygotic twins so that better growth performance and carcass traits can be translated to the increased production of beef with higher economic value. Several studies have reported that monozygotic twins are produced by embryo bisection. On the other hand, with the evolution and stabilization of in vitro fertilization technology, it has become possible to produce multiple monozygotic twin calves from blastomeres separated from a cleavage-stage embryo. This review attempts to clarify breeding practices through revalidation of the factors that affect the production efficiency of monozygotic twin calves by embryo bisection. Furthermore, the establishment of a system for monozygotic twin embryo production via the simplified technique of blastomere separation is reviewed while showing data from our previously performed studies.

show abstract

Section: Establishment Of Efficient Production System For Monozygoticmentioning

confidence: 99%

The contribution of efficient production of monozygotic twins to beef cattle breeding

Hashiyada

2017

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

“…Another important factor influencing the developmental ability of nuclear transferred eggs is the combination of the cell cycle of the donor nucleus and recipient cytoplasm [18]. Aono et al [19] reported that the developmental ability of nuclear transferred bovine oocytes to blastocysts receiving a blastomere from in vitro fertilized embryos cultured for 116 h was significantly higher than that from embryos cultured for 68, 92 or 140 h. They suggested that such a difference occurred because the cell cycle of the donor nucleus was at a different stage. In the present study, the donor nucleus (blastomere) was fused with enucleated oocytes which were previously parthenogenetically activated.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Age of Donor Embryos on the Developmental Ability of Bovine Nuclear Transferred Eggs In Vitro.

Ohkoshi

Hara

Kato

et al. 1997

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. The effect of the age of a donor embryo on the developmental ability of bovine nuclear transferred eggs was examined. A blastomere of an in vitro fertilized embryo cultured for 3.5 to 6.5 days, or an inner cell mass cell (ICM) from a blastocyst cultured for 7.5 days was fused with an enucleated, preactivated oocyte matured in vitro by electrical stimulation. Fused eggs were cocultured with cumulus cells for 10 days in vitro. The fusion rate of blastomeres from day 3.5 to 6.5 embryos with enucleated oocytes (78 to 88%) and the developmental ability of nuclear transferred eggs to blastocysts (6 to 10%) were not significantly affected by the age of the donor embryo. However, the fusion rate of the ICM cell was significantly low (28%). Although nuclear transferred eggs receiving an ICM cell developed to 2-cell (73%) and 8-cell (22%) stages at the same rates as those obtained in other groups (68 to 81% for 2-cell, 30 to 34% for 8-cell), none of them developed to blastocysts.

show abstract

“…The use of activated oocytes reduced the frequency of abnormal karyomorphism of NT embryos (1/46 vs. 17/56, P<0.01), and the productivity of NT embryos was further increased by (1) the use of a mix activation method for parthenogenetic activation of un-aged oocytes [35] and (2) synchronizing the cell cycle between donor nuclei and an activated recipient ooplasm [36]. The improvements enabled by these developed techniques subsequently allowed the production of approximately six cloned embryos from a single donor embryo [37].…”

Section: Application Of the Developmental Engineering Utilizing The Tmentioning

confidence: 99%

Application Study of Developmental Engineering for Livestock Production

Ushijima

2005

J. Reprod. Dev.

View full text Add to dashboard Cite

Abstract. This paper describes several technical improvements in developmental engineering for livestock production, including their practical utility in the field. The artificial production of monozygotic twins via embryo splitting is shown to increase embryo productivity, while embryo sexing capability provides added value without compromising offspring productivity, with both techniques being adequate for practical field applications. It is also shown that: (1) the development of nuclear transfer utilizing oocytes collected from slaughtered ovaries and matured in vitro enables producing a large number of cloned embryos, (2) the intracytoplasmic injection of somatic cell improves the productivity of nuclear transplantation, and (3) the injection of sperm increases the rate of normal oocytes with male and female pronuclei allowing further preimplantation development. Finally, the removal of cytoplasmic lipid droplets from embryos following centrifugation alters an embryo's intrinsic sensitivity to low temperature allowing long-term preservation. Collectively, these techniques have clearly provided improvements in developmental engineering for livestock production.

show abstract

Effect of Donor Embryo Stage on Development of Nuclear Transplants in Cattle

Cited by 3 publications

References 12 publications

The contribution of efficient production of monozygotic twins to beef cattle breeding

The contribution of efficient production of monozygotic twins to beef cattle breeding

Effects of the Age of Donor Embryos on the Developmental Ability of Bovine Nuclear Transferred Eggs In Vitro.

Application Study of Developmental Engineering for Livestock Production

Contact Info

Product

Resources

About