Effect of domestic cooking on human bioavailability of naringenin, chlorogenic acid, lycopene and ?-carotene in cherry tomatoes

Bugianesi, Rossana; Salucci, M; Leonardi, C.; Ferracane, Rosalia; Catasta, Giovina; Azzini, Elena; Maiani, Giuseppe

doi:10.1007/s00394-004-0483-1

Cited by 116 publications

(83 citation statements)

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“…Several studies have shown significant effects of treatment with a small number of subjects (Hollman et al 1996;McAnlis et al 1999;Cremin et al 2001;Rechner et al 2001a;Olthof et al 2001;Bugianesi et al 2004). …”

Section: Subjectsmentioning

confidence: 99%

“…In all plasma samples, the following target compounds: CGA, CA, DHCA, FA, DHFA, apigenin and luteolin were analyzed in duplicate with and without enzymatic hydrolysis to detect free and conjugated form. Single phenol levels were evaluated by HPLC with electrochemical detector before and after enzymatic hydrolysis as described by Bugianesi et al (2004) with slight modification. Briefly for free forms, 100 ml ascorbic acid solution (1 %) was added to 0·5 ml plasma and after vortexing and protein precipitation by 500 ml 3 mol/l HCl-MeOH (1:1, v/v), free phenols were extracted by adding 2 ml ethyl acetate, followed by stirring and sonication (2 -3 min) before centrifugation at 1700 g for 5 min.…”

Section: Plasma Samplesmentioning

confidence: 99%

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Absorption and metabolism of bioactive molecules after oral consumption of cooked edible heads ofCynara scolymusL. (cultivar Violetto di Provenza) in human subjects: a pilot study

et al. 2007

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The current growing interest for natural antioxidants has led to a renewed scientific attention for artichoke, due not only to its nutritional value, but, overall, to its polyphenolic content, showing strong antioxidant properties. The major constituents of artichoke extracts are hydroxycinnamic acids such as chlorogenic acid, dicaffeoylquinic acids caffeic acid and ferulic acid, and flavonoids such as luteolin and apigenin glycosides. In vitro studies, using cultured rat hepatocytes, have shown its hepatoprotective functions and in vivo studies have shown the inhibition of cholesterol biosynthesis in human subjects. Several studies have shown the effect on animal models of artichoke extracts, while information on human bioavailability and metabolism of hydroxycinnamates derivatives is still lacking. Results showed a plasma maximum concentration of 6·4 (SD 1.8) ng/ml for chlorogenic acid after 1 h and its disappearance within 2 h (P, 0·05). Peak plasma concentrations of 19·5 (SD6·9) ng/ml for total caffeic acid were reached within 1 h, while ferulic acid plasma concentrations showed a biphasic profile with 6·4 (SD1·5) ng/ml and 8·4 (SD4·6) ng/ml within 1 h and after 8 h respectively. We observed a significant increase of dihydrocaffeic acid and dihydroferulic acid total levels after 8 h (P,0·05). No circulating plasma levels of luteolin and apigenin were present. Our study confirms the bioavailability of metabolites of hydroxycinnamic acids after ingestion of cooked edible Cynara scolymus L. (cultivar Violetto di Provenza).

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Section: Subjectsmentioning

confidence: 99%

Section: Plasma Samplesmentioning

confidence: 99%

Absorption and metabolism of bioactive molecules after oral consumption of cooked edible heads ofCynara scolymusL. (cultivar Violetto di Provenza) in human subjects: a pilot study

et al. 2007

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“…Epidemiological studies have consistently shown an inverse association between consumption of some nutritional flavonoids and the risk of human cancers at many sites [6][7][8][9]. NAR has been widely studied and has been reported to be an antioxidant [10,11] …”

Section: Introductionmentioning

confidence: 99%

Formulation and Evaluation of Polymeric Nanosuspension of Naringenin

Sumathi¹,

Sengodan²,

Sivakumar³

2017

Int J App Pharm

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, S. TAMIZHARASI, T. SIVAKUMAR Nandha College of Pharmacy and Research Institute, Erode 630052, Tamilnadu, IndiaEmail: sumoraji@gmail.comObjective: The objective of this study was to formulate and evaluate the poorly soluble drug, naringenin (NAR) into nanosuspension to increase the solubility and enhance the dissolution rate and then improve its bioavailability.Methods: Nanosuspenion of naringenin (NARNS) was prepared using high-pressure homogenization method using Soya lecithin, Polaxamer-407, Polaxamer-188, Hydroxypropyl methyl cellulose (HPMC) and Tween-80. Ten formulations were prepared to show the effect of stabilizer and its ratio. D-α-Tocopheryl polyethene glycol succinate 1000 (TPGS) was added as a co-stabilizer. All these formulations were evaluated for their particle size, PDI, zeta potential, FT-IR study, drug content, saturation solubility studies, entrapment efficiency, in vitro permeability and in vitro drug release. The formulation was further evaluated for scanning electron microscope (SEM), differential scanning calorimetry (DSC) and Powder X-ray diffraction (P-XRD) and hemocompatibility assessment.Results: All the prepared formulations were in the nano size. The optimum concentration of the stabilizer was in the formulation was found 1:1.5:1 (drug: stabilizer: co-stabilizer ratio). Dramatic effect of the particle size reduction was found by the addition of the co-stabilizer (TPGS) in formulation N2 that has P. S 80.52±0.13 nm. The solubility and dissolution of NAR in the form of NARNS were significantly higher than those of pure NAR. SEM report shows that naringenin nanosuspension revealed a smooth texture. P-XRD crystallography diffraction and DSC studies indicated that the crystalline state of NAR was converted into amorphous nature. The safety evaluation showed that NARNS provided a lower rate of erythrocyte hemolysis.Keywords: Naringenin, High-Pressure Homogenization, Nanosuspenion, Solubility, Bioavailability Conclusion:In this study, (NARNS) was successfully carried out by high-pressure homogenization technique and characterized. The physiochemical characterization shown that crystalline naringenin was converted to a polymorphic form (DSC and P-XRD Study) which evidenced by enhanced dissolution rate in comparisons of the formulation with (NAR) pure drug. The NARNS has shown 7.5±0.4 fold increased relative bioavailability when compared to the NAR. The increased drug dissolution rate may have a significant impact in absorption which in turn the improved oral bioavailability of naringenin. Thus, this delivery system may prefer to improve the dissolution of poorly soluble drugs like NAR and thus enhanced oral bioavailability. The safety evaluation showed that nanoformulation (NF2) shows a lower rate of erythrocyte hemolysis. These findings suggest that the selected formulation may represent a promising new drug formulation for intravenous administration in the treatment of certain cancers.

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“…L y c o p e n e a b s o r p t i o n h a s b e e n s h o w n t o b e a f f e c t e d b y t h e p r e s e n c e o f o t h e r carotenoids, the lipid status, and plasma antioxidant capacity (Bohm & Bitsch, 1999). However, another study found that lycopene plasma levels after consumption of cherry tomatoes are insignificantly different from the plasma base line (Bugianesi et al, 2004). Further absorption from micelles has been shown to be slow (e.g.…”

Section: Kinetics Of Phytochemical Bioavailabilitymentioning

confidence: 92%