Effect of diquat on cell growth and macromolecule synthesis in cultured pneumocytes.

Abstract

The effects of diquat (DQ) on cell growth and macromolecule synthesis were investigated in cultured rat, feline and human. pneumocytes. DQ at 10-3 M and 10-5 M showed time-and dose-dependent inhibition of cell growth for 7 days. The incorporation of 3H-thymidine, 3H-uridine and 14C-leucine into DNA, RNA and protein was reduced to 0-47% by 10-3 M of DQ, and that of 14C-leucine into protein of A-549 cells was reduced to 67% by 10-5 M of DQ. Among the cells examined, A-549 cells derived from human lung were the most resistant to the inhibitory effects of DQ on cell growth and macromolecule synthesis. On the other hand, human lung embryonic fibroblast cells and L-2 cells derived from rat lung were the most sensitive to the toxicity of DQ. These results indicate that the inhibitory effects of DQ on cell growth and on macromolecule synthesis are dependent on the concentrations of DQ administrated, and that species differences in the sensitivity to DQ toxicity may exist.

Systemic responses of weaned rats to spermine against oxidative stress revealed by a metabolomic strategy

“…The above results agree with those of a previous study, which found that diquat can reduce protein synthesis in cells. 38 As a result, amino acids decreased during protein synthesis and thus caused decreased levels of the amino acids present in plasma. In this study, levels of plasma glutamate, and lysine were decreased, consistent with the function of diquat in reducing the protein synthesis in rats.…”

Continuous in vivo infusion of interferon-gamma (IFN-γ) preferentially reduces myeloid progenitor numbers and enhances engraftment of syngeneic wild-type cells in Fancc-/- mice

“…[21][22][23][24][25] IFN-␥ has been used clinically to treat a number of malignancies (reviewed in Liles 23 and Borden 24 ) and has a high therapeutic index. 25 Previously, in vitro studies in 2 murine models of Fancc, 26,27 as well as cells from FA type C patients, 28 established that cells lacking functional Fancc were hypersensitive to IFN-␥.…”