Effect of diode laser irradiation on the survival rate of gingival fibroblast cell cultures

Kreisler, Matthias; Daubländer, Monika; Willershausen‐Zönnchen, B.; dʼHoedt, B

doi:10.1002/lsm.1072

Cited by 151 publications

(12 citation statements)

References 13 publications

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“…The authors found that the shorter the time of exposure to the laser, the greater the proliferation of fibroblasts. Likewise, Kreisler et al 25 also consider the time of exposure to laser radiation important for the stimulation of fibroblast proliferation, which is even more relevant than the actual laser power.…”

Section: Final Considerationsmentioning

confidence: 99%

Efeitos bioestimulantes do laser de baixa potência no processo de reparo

Lins

Dantas

Lucena

et al. 2010

An. Bras. Dermatol.

138

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Abstract:The wound healing process has always been an excellent subject for researchers. The use of lowpower laser on wounds during the postoperative phase has increased the speed of the healing process. It has been implied that low power radiation affects cellular metabolic processes and promotes beneficial biological effects (analgesic, anti-inflammatory, and healing). Laser biostimulation appears to influence the behavior of the repair process. This paper aims at reviewing the most interesting aspects of the use of lowpower laser in the tissue-repair process. Key words: lasers, low-level laser therapy, wound healing Resumo: Os lasers de baixa potência promovem efeitos biológicos benéficos, de caráter analgésico, antiinflamatório e cicatrizante, por meio de um fenômeno de bioestimulação. A radiação emitida pelo laser terapêutico afeta os processos metabólicos das células-alvo, produzindo efeitos bioestimulantes que resultam na ocorrência de eventos celulares e vasculares, os quais parecem interferir diretamente no processo de reparo. Este trabalho visa estudar o fenômeno da bioestimulação e destacar os principais efeitos bioestimulantes do laser de baixa potência na reparação tecidual. Palavras-chave: Cicatrização de feridas; Lasers; Terapia a laser de baixa intensidade

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Section: Final Considerationsmentioning

confidence: 99%

Efeitos bioestimulantes do laser de baixa potência no processo de reparo

Lins

Dantas

Lucena

et al. 2010

An. Bras. Dermatol.

138

View full text Add to dashboard Cite

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“…12,[18][19][20] In others, the cells were seeded in Petri dishes and the probe was positioned at a distance of 9 cm 1 or 13 cm 21 from the cell cultures. Cells seeded in wells of 24 well plates were also irradiated at a distance of 0.5 mm 22 or 550 mm. 15 There is a significant difference in experimental methodologies when only one light source (serial procedure) has been used to irradiate a 96 well and a 24 well test plate or a Petri dish, especially when comparing the delay between irradiating the first and last sample of plate, or a limitation of the maximum power density reached in experimental protocol, because of the need to irradiate the entire area of the well bottom.…”

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confidence: 99%

A Novel 785-nm Laser Diode-Based System for Standardization of Cell Culture Irradiation

Lins

Oliveira

Guimarães

et al. 2013

Photomedicine and Laser Surgery

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Objective: The purpose of this study was to develop a novel device that concatenates alignment of infrared lasers and parallel procedure of irradiation. The purpose of this is to seek standardization of in vitro cell irradiation, which allows analysis and credible comparisons between outcomes of different experiments. Background data: Experimental data obtained from infrared laser therapies have been strongly dependent upon the irradiation setup. Although further optical alignment is difficult to achieve, in contact irradiation it usually occurs. Moreover, these methods eventually use laser in a serial procedure, extending the time to irradiate experimental samples. Methods: A LASERTable (LT) device was designed to provide similar infrared laser irradiation in 12 wells of a 24 well test plate. It irradiated each well by expanding the laser beam until it covers the well bottom, as occurs with unexpanded irradiation. To evaluate the effectiveness of this device, the spatial distribution of radiation was measured, and the heating of plain culture medium was monitored during the LT operation. The irradiation of LT (up to 25 J/cm 2 -20 mW/cm 2 ; 1.250 sec) was assessed on odontoblast-like cells adhered to the bottom of wells containing 1 mL of plain culture medium. Cell morphology and metabolism were also evaluated. Results: Irradiation with LT presented a Gaussian-like profile when the culture medium was not heated > 1°C. It was also observed that the LT made it 10 times faster to perform the experiment than did serial laser irradiation. In addition, the data of this study revealed that the odontoblast-like cells exposed to low-level laser therapy (LLLT) using the LT presented higher metabolism and normal morphology. Conclusions: The experimental LASERTable assessed in this study provided parameters for standardization of infrared cell irradiation, minimizing the time spent to irradiate all samples. Therefore, this device is a helpful tool that can be effectively used to evaluate experimental LLLT protocols.

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“…Providing laser treatment at low energy levels can be useful and beneficial for healing and regeneration, the predominance of literature suggests that LLLT occurs with visible and near-infrared wavelengths from 633 to 904nm [36][37][38]. Diode laser would be better for biostimulation due to less wavelength than the Er:YAG Laser.…”

Section: Discussionmentioning

confidence: 99%

Multiple Applications with Er:YAG Laser in Apicoectomy - A Clinical Case Report

Yu¹

2017

Dentistry

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The setting was 50 mJ/10 Hz with 100% of water for full mouth laser scaling and then ultrasonic scaling tip was used for supragingival scaling. In addition, another laser setting was applied at 50 mJ/30Hz with 100% of water for subgingival sterilization and then ultrasonic round tip was used for subgingival curettage [7,8]. Step 2. Vertical soft tissue incisionThe setting was 100 mJ/40 Hz with 100% of water for incision [in vestibular near apex of #21] (Figure 3). Step 3. Granulation tissues ablationThe setting was 400 mJ/15Hz with 100% of water. Granulation tissues were ablation thoroughly (Figures 4 and 5).

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Effect of diode laser irradiation on the survival rate of gingival fibroblast cell cultures

Cited by 151 publications

References 13 publications

Efeitos bioestimulantes do laser de baixa potência no processo de reparo

Efeitos bioestimulantes do laser de baixa potência no processo de reparo

A Novel 785-nm Laser Diode-Based System for Standardization of Cell Culture Irradiation

Multiple Applications with Er:YAG Laser in Apicoectomy - A Clinical Case Report

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