Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depikRasbora tawarensis(Pisces: Cyprinidae) eggs after short‐term cryopreservation

Abstract

Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non‐permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non‐permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservation of Rasbora tawarensis sperm. A completely randomized experimental design was used in this study which involves two types of cryoprotectant and their combination at different concentrations, namely 5% DMSO, 5% egg yolk, 5% DMSO + 5% egg yolk and 2.5% DMSO + 2.5% egg yolk. Every treatment was conducted in three replicates. Combination of 5% DMSO + 5% egg yolk gave the best results cryoprotectant treatment had significant effects on sperm motility, fertilization and hatching rate of the R. tawarensis eggs (p < .05). It is concluded that the best proportion of cryoprotectants for sperm cryopreservation in this species is 5% DMSO + 5% egg yolk.

Vital Analysis of Cryopreserved Sperm of Marbled Flounder, Pseudopleuronectes yokohamae

“…% DNA in tail 0.6 ± 0.1 a 0.7 ± 0.1 a 0.9 ± 0.1 a 0.9 ± 1.2 a 0.9 ± 1.2 a Tail moment (µ) 0.1 ± 0.0 a 0.1 ± 0.0 a 0.1 ± 0.0 a 0.1 ± 0.0 a 0.1 ± 0.0 a The present study revealed that extender-3 was not suitable for the cryopreservation of marbled flounder sperm. However, it has been previously reported that extender-3 is suitable for cryopreserving sperm of Rasbora tawarensis (Muchlisin et al, 2020) and yellow catfish, Pelteobagrus fulvidraco (Pan et al, 2008). The present findings suggest that the absence of sugar in extender-3 might be responsible for the reduced motility of postthaw sperm, although a positive impact of sugar-based extenders (extender-1 and extender-2) on the sperm cryopreservation has been described in the previous section because such extender can additionally act as a non-penetrating CPA (Rusco et al, 2019).…”

Effect of cryoprotectant on the motility, viability, fertilization, and DNA integrity of naleh fish Barbonymus sp. (Cyprinidae) sperm

“…Thus, the fresh sperm were characterized based on pH, color, and consistency, and also for initial quality (motility and viability). Also, only sperm with minimum motility above 60% were used for cryopreservation (Muchlisin et al, 2020).…”

“…In cryopreservation, cryoprotective agent (CPA) is known to play an important role during this process, and therefore, it must be known which type of CP is suitable for species to species, this is because the CPA is species dependent (Chao & Liao, 2001;Sarder et al, 2012). There are generally two types of CPA, intracellular (ICPA) and extracellular (ECPA) (Muchlisin, 2005;Muchlisin et al, 2020). The ICPA possesses the capacity to penetrate sperm cells and confer internal protection through the formation of ice crystals, while the latter confers an external shield from temperature shock, both during cryopreservation and thawing (Muchlisin et al, 2020).…”

“…There are generally two types of CPA, intracellular (ICPA) and extracellular (ECPA) (Muchlisin, 2005;Muchlisin et al, 2020). The ICPA possesses the capacity to penetrate sperm cells and confer internal protection through the formation of ice crystals, while the latter confers an external shield from temperature shock, both during cryopreservation and thawing (Muchlisin et al, 2020). Some of the commonly used intracellular CPAs include DMSO (dimethylsulfoxide), glycerol, methanol, ethanol, and ethylene glycol, while egg yolk, honey, and glucose have been adopted as extracellular.…”

“…Several studies demonstrated better output while using a combination of both types, compared to an individual application. For example, Abinawanto et al (2013Abinawanto et al ( , 2018 used a combination of methanol and egg yolk for botia Chrombotia macracanthus sperm and Java barb Barbonymus gonionotus, while Muchlisin et al (2020) used a combination of egg yolk and DMSO for depik fish Rasbora tawarensis spermatozoa. Currently, there is no information on the preferred combination of ICPA and ECPA for naleh fish spermatozoa.…”