Effect of cryoprotectant on the motility, viability, fertilization, and DNA integrity of naleh fish Barbonymus sp. (Cyprinidae) sperm

Abstract

Naleh fish Barbonymus sp. is a commercial freshwater fish, which is indigenous to Aceh, Indonesia. The population of this species has declined over the years as a result of habitat perturbations and overfishing. Hence, the crucial need to develop a cryopreservation method to support breeding programs. This involved the use of a cryoprotectant as an important component. The objective of this study, therefore, was to explore the best cryoprotectant for naleh fish spermatozoa, and a total of five types were tested. These include the DMSO, Methanol, Ethanol, Glycerol, and Ethylene Glycol at a similar concentration of 10%, which were individually combined with 15% egg yolk, and every treatment was performed in three replications. Conversely, Ringer’s solution was adopted as an extender, and the sperm was cryopreserved in liquid nitrogen for 15 days. The results showed significant influence on sperm motility and viability, as well as egg fertility of naleh fish (P <0.05), although the DMSO provided the best outcome, compared to others at 47.17%, 50.13%, and 45.67%, respectively. Furthermore, DNA fragmentation had not occurred in the fresh and cryopreserved sperm samples, indicating the protective effect of tested cryoprotectants. It is concluded that the 10% DMSO and 15% egg yolk is the best cryoprotectant for naleh fish spermatozoa.

The effect of cryoprotectant on the viability and motility of goldfish Carassius aurtus sperm after 48 hours refrigerated

“…Egg yolk protein and lecithin function to protect sperm from damage caused by temperature shock by maintaining the integrity of lipoproteins in the plasma membrane of spermatozoa (Zambrowicz et al, 2014;Sunwoo and Gujral 2015). Therefore, the use of intracellular and extracellular cryoprotectants provides optimal protection as recorded in this study, the similar finding was also reported by several researchers (Muthmainnah et al, 2018;Muchlisin et al, 2020;Maulida et al, 2021).…”

“…The effectiveness of DMSO as a cryoprotectant has been reported in several studies. Maulida et al (2021) reported that it was effective for preserving naleh fish Barbonymus sp. sperm.…”

“…Meanwhile, extracellular cryoprotectant has a large molecular weight and size that prevents it from passing through the sperm cell membrane, thereby protecting the cell from outside, particularly from temperature shock and the toxic effects of diluent and cryoprotectant (De-Andrade et al, 2014;Muchlisin et al, 2015a;Muchlisin et al, 2015b;Maulida et al, 2020). The commonly used intracellular cryoprotectant includes Dimethyl sulfoxide (DMSO), Glycerol, Methanol, Ethanol, and Ethylene Glycol (Abinawanto et al, 2012;Abinawanto et al, 2013;Muchlisin et al, 2015a;Maulida et al, 2021). Meanwhile, the popular extracellular cryoprotectant includes honey, glucose, skim milk, egg yolk, coconut water, soybean milk, and lactose (Abinawanto et al, 2013;Bozkurt et al, 2014;Eriani et al, 2017;Muchlisin et al, 2020;Abinawanto et al, 2021).…”

The effect of extender on the quality of climbing perch ( Anabas testudineus ) sperm after refrigerated storage

“…However, several fish species have shown a slightly acidic semen pH, such as common carp Cyprinus carpio L. (Pradana et al, 2020) and Barbonymus sp. (Maulida et al, 2021). Some other fish species have alkaline sperm, such as Schizothorax progastus (Agarwal et al, 2013), Schizothorax richardsonii (Raghuvanshi et al, 2019) and Labeo rohita (Bibi et al, 2021).…”

Acoustic-mechanical effect on the sperm of sturgeon fish using piezoactuators

“…Over the past decade, significant progress has been made in the process of cryopreservation of fish sperm, and, as a result, fertilization. Progress was associated with the use of new cryopreservation methods, including the search for new tread compositions [7][8] and high-speed freezing modes [9], which lead to better cryo-survival of spermatozoa.…”