Effect of Diluents for Cold Storage of Olive Flounder (Paralichthys olivaceus) Sperm

Role of antibiotics on chilled storage sperm motility of waigieu seaperch Psammoperca waigiensis (Cuvier and Valencienes, 1828)

“…The results indicate that the optimal condition for chilled storage Waigieu seaperch semen is artificial seminal plasma extender diluted at 1:3 (semen: ASP), supplemented with 200ppm gentamycin and stored at 2 o C. Discussion The motility and velocity of sperm of waigieu seaperch changed in chilled storage, indicating changes in sperm quality. Sperm quality is most accurately measured by fertilization percentage (Lim et al, 2005;Le et al, 2011). This method could not be used in our experiments because females of Waigieu seaperch did not produce mature eggs until the experiments were completed.…”

“…This method could not be used in our experiments because females of Waigieu seaperch did not produce mature eggs until the experiments were completed. When fertilization trials could not be performed, motility and velocity of sperm were used as an indicator of sperm quality (Lim et al, 2005(Lim et al, , 2006Le et al, 2011). Addition of antibiotics to undiluted semen and extender improve storage duration (Billard et al 2004;Bobe and Labbe 2009;Le et al 2011).…”

“…It reduces the need of frequent collection of milt from males, enables transportation of sperm to distant locations and prevents problems related to asynchrony in gamete production between males and females. Semen storage is affected by extenders, dilution ratio, temperature, and antibiotics (Le et al 2011;Lim et al 2006;Lim et al 2005). The presence of microorganisms in stored samples may endanger valuable germplasm by decreasing fertilization and lowering cell and viability (Segovia et al 2000).…”

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

“…In practice, cold storage of sperm promotes egg production in hatcheries because it is a low-cost and easy means of overcoming the insufficient number of males. The effect of short-term storage of fish sperm has been evaluated in marine fish species such as olive flounder (Paralichthys olivaceus) [5], marbled sole (Limanda yokohamae), brown sole (Limanda herzensteini) [6], starry flounder (Platichthys stellatus) [7], yellow croaker (Larimichthys polyactis) [8], and giant grouper (Epinephelus lanceolatus) [9]. However, to our knowledge, no study has evaluated the influence of short-term preservation of spotted halibut sperm on sperm function.…”

“…The successful storage of fish sperm depends on numerous factors, including extenders, dilution ratio, temperature, and antibiotics [5,7]. During storage, sperm motility, fertilization capacity, cell viability, and energy availability for sperm activation must be maintained [10].…”