Effect of Different Wound Dressings on Cell Viability and Proliferation

Paddle-Ledinek, Joanne E.; Nasa, Zeyad; Cleland, Heather

doi:10.1097/01.prs.0000225439.39352.ce

Cited by 180 publications

(124 citation statements)

References 21 publications

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“…Based on the MTT data one could expect approximately 100% cell death, which was supported by light microscope pictures. This is also supported by previous work showing that silver ions are toxic to skin cells in vitro (Barnea, Weiss & Gur, 2010;Burd et al, 2007; Paddle- Ledinek, Nasa & Cleland, 2006;Poon & Burd, 2004).…”

Section: Discussionsupporting

confidence: 76%

“…Our data are in consistence with the work done by Čolić et al (Čolić, Mihajlović, Mathew, Naseri & Kokol, 2015) showing reduced metabolic activity and cell proliferation, but low cytotoxicity for CNF. Also AquaCel ® has previously been shown to give a reduction in cell proliferation, but not affecting survival in keratinocytes (Paddle-Ledinek, Nasa & Cleland, 2006). CNF dispersion (50 µg/ml) did not affect the fibroblasts or the keratinocytes during the 24 h incubation, showing that a potential leakage of CNFs in a wound could be acceptable.…”

Section: Discussionmentioning

confidence: 97%

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Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Nordli

Chinga-Carrasco²,

Rokstad

et al. 2016

Carbohydrate Polymers

125

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 Cellulose nanofibril dispersion (50 µg/ml) did not affect the cytotoxicity or metabolic activity of Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes  Aerogels made of cellulose nanofibrils induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death  Cytokine profiling measuring 27 cytokines revealed that the keratinocytes and fibroblasts did not induce cytokines upon direct exposure to the CNF materials. Due to the nano dimension of the CNFs, the aerogels had a high moisture-holding capacity (~7500%) 3 AbstractWood cellulose nanofibrils have been suggested as a potential wound healing material, but its utilization is limited by FDA requirements regarding endotoxin levels. In this study a method using sodium hydroxide followed by TEMPO mediated oxidation was developed to produce ultrapure cellulose nanofibrils (CNF), with an endotoxin level of 45 endotoxin units/g (EU/g) cellulose. Scanning transmission electron microscopy (S(T)EM) revealed a highly nanofibrillated structure (lateral width of 3.7±1.3 nm).Assessment of cytotoxicity and metabolic activity on Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes was done. CNF-dispersion of 50 g/ml did not affect the cells. CNF-aerogels induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death. Cytokine profiling revealed no induction of the 27 cytokines tested upon exposure to CNF. The moisture-holding capacity of aerogels was relatively high (~7500%), compared to a commercially available wound dressing (~2500%),indicating that the CNF material is promising as dressing material for management of wounds with a moderate to high amount of exudate.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 97%

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Nordli

Chinga-Carrasco²,

Rokstad

et al. 2016

Carbohydrate Polymers

125

View full text Add to dashboard Cite

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“…Owing to its antibacterial property, silver is used for the production of SiNPs containing medical products, such as wound dressings, devices, and catheters, to lower the incidence of bacterial infections. 13 However, Paddle-Ledinek et al 14 found that extracts from wound dressings containing SiNPs were more toxic to keratinocytes among those nanomaterials tested. SiNPs are well known to be toxic to various tissues, such as lung, liver, brain, vessels, and reproductive organs.…”

mentioning

confidence: 99%

In vivo toxicologic study of larger silica nanoparticles in mice

Chan¹,

Liu²,

Chiau³

et al. 2017

IJN

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Silica nanoparticles (SiNPs) are being studied and used for medical purposes. As nanotechnology grows rapidly, its biosafety and toxicity have frequently raised concerns. However, diverse results have been reported about the safety of SiNPs; several studies reported that smaller particles might exhibit toxic effects to some cell lines, and larger particles of 100 nm were reported to be genotoxic to the cocultured cells. Here, we investigated the in vivo toxicity of SiNPs of 150 nm in various dosages via intravenous administration in mice. The mice were observed for 14 days before blood examination and histopathological assay. All the mice survived and behaved normally after the administration of nanoparticles. No significant weight change was noted. Blood examinations showed no definite systemic dysfunction of organ systems. Histopathological studies of vital organs confirmed no SiNP-related adverse effects. We concluded that 150 nm SiNPs were biocompatible and safe for in vivo use in mice.

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“…An optimum microenvironment and absence of cytotoxic factors are essential for the phase of epithelization of the wound healing process. In-vitro studies show that Acticoat is toxic to both keratinocytes and fibroblasts, suppressing proliferation at the same time (Andrew et al, 2005;Burd et al, 2007;Paddle-Ledinek et al, 2006). The monitored patients' clinical results do not demonstrate such an in-vitro effect.…”

Section: Discussionmentioning

confidence: 99%