Effect of different liquid cultures of live yeast strains on performance, ruminal fermentation and microbial protein synthesis in lambs

Abstract

Three yeast strains, Kluyveromyces marximanus NRRL-3234 (KM), Saccharomyces cerevisiae NCDC-42 (SC) and Saccharomyces uvarum ATCC-9080 (SU), and a mixed culture (1:1:1 ratio) were evaluated for their value as probiotics in lamb feeding in two experiment. In experiment I and II, 20 and 30 pre-weaner lambs were fed for 63 and 60 days in two and three equal groups respectively. All lambs were offered ad libitum a creep mixture and Zizyphus nummularia leaves, and yeasts were dosed orally. In experiment I, one group received no yeast, the other of the mixed culture (1.5-2 x 10(10) live cells/ml). In experiment II, yeast cultivation was modified yielding 1.5-2 x 10(13) live cells/ml. Lambs of the three experimental groups received 1 ml/kg live weight of one of the individual yeasts. Feed intake did not differ among groups of both experiments with the exception of SC-supplemented lambs in experiment II which showed a trend to higher intakes per kg metabolic body weight and in percentage of body weight when compared with KM- and SU-supplemented lambs. Supplementation of the mixed yeast culture had no effect on intakes of digestible crude protein and metabolisable energy, nutrient digestibility, nitrogen balance and rumen fermentation characteristics (pH, ammonia, volatile fatty acid concentration, protozoa count) and urinary allantoin as an indicator of microbial protein synthesis. The same was true for comparisons in experiment II except ciliate protozoa counts, which showed a trend to be the highest with SU and the lowest with SC. The results of present study show that the response of lambs to supplemented live yeast cultures is inconsistent, as it lacked to have an effect in the present study, and that differences among strains were small, even when supplemented at a much higher live cell count.

The effect of live Saccharomyces cerevisiae yeast in the diet of rams on the digestibility of nutrients, nitrogen and mineral retention, and blood serum biochemical parameters

“…Marden et al (6) observed increased total tract digestibility of NDF and ADF when dairy cows were fed a diet with live yeast. In contrast to the cited authors, Tripathi et al (7) showed that neither NDF nor ADF were influenced when the diet was supplemented with live S. cerevisiae. The yeast additive can reduce lactate accumulation in the rumen (6) by increasing the count of lactate-utilizing bacteria, particularly Selenomonas ruminantium, and also by decreasing the number of the lactate-producing bacteria, Streptococcus bovis (3).…”

“…In the current study, the total tract digestibility of other nutrients did not increase when yeast was added to the diet. Most literature data show no improvement in digestibility of dry matter and organic matter when live yeast is added to the ration (7,16,17). In several trials, higher dry and organic matter digestibility was reported (6,18) when live S. cerevisiae cells were supplemented, due to the increased structural carbohydrate digestibility.…”

Role of probiotics in nutrition and health of small ruminants

“…Several microbial species, mainly bacteria (lactic acid and non-lactic acid bacteria), yeasts (dairy strains) or fungi are considered as probiotics (Tripathi et al 2008).…”

“…Some researchers have found no effect in rumen pH of small ruminants (Doreau et al 1998, Tripathi et al 2008), whilst others have recorded an increase in rumen pH (Radev 1999, Abd El-Ghani 2004. In contrast, Kowalik et al (2011) and Tripathi and Karim (2011) have reported a reduction in rumen pH after supplementation with Saccharomyces uvarum (ATCC9080; SU) or an equal mixture of Kluyveromyces marximanus (NRRL3234; KM), Saccharomyces cerevisiae (NCDC42; SC) and S. uvarum (ATCC9080; SU) to growing lambs.…”

Effect of Celmanax on feed intake, live weight gain and nematode control in growing sheep

“…Increased live weight gain and feed conversion upon supplementation with Celmanax ® was associated to improved efficiency of fermentation (stimulation of microbial growth, activity and increased proportion of available carbon towards microbial protein synthesis), that might have diverted balanced supply of nutrient towards cell growth Tripathi and Karim, 2011). Others have observed increased live weight gain and feed conversion due to supplementation with yeast (Abdelrahman and Hunaiti, 2008;Lesmeister et al, 2004;Tripathi and Karim, 2011), while others have not (Titi et al, 2008;Tripathi et al, 2008). This study demonstrated that Celmanax® supplementation have the potential of improving intake, live weight gain and feed conversion efficiency, hence its application as possible feed additives in small ruminants.…”