Effect of different growth regulators and genotype onin vitropropagation of jojoba

Abstract

Explants of jojoba (Simmondsia chinensis (Link) Schn.) from randomly selected plants were propagated in vitro. On modified Murashige-Skoog (MS) medium supplemented with 1 mg/litre BA (N 6 -benzyl-adenine) there was a 4.6-fold increase in shoot numbers at 30 days. After 15 days on modified MS medium supplemented with 3 mg/litre IBA (indolebutyric acid) c. 25% of the shoots had developed roots. The best exflasking survival rate of the rooted plantlets (90%) was observed when the plantlets were transferred to an organic mixture, Agrosoil™. Subsequent growth rates in Agrosoil™ were higher than in other potting media tested. On the other hand, the response of each clone was highly variable, some exhibiting 75% root formation at 60 days while others displayed little response.

Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

“…All the examined concentrations of BA produced short were primary shoots and a smaller number of nodes per primary shoot than those observed in MS. The results of the present study are in agreement with those reported by Llorente and Apostolo (1998). They reported that there was a 4.6-fold increase in shoot numbers of jojoba at 30 days on MS supplemented with 1 mg/l BA.…”

“…Moreover, jojoba plants derived from tissue culture grow more vigorously than seedlings or rooted cuttings, and yield a significantly larger population after first year growth. Some reports on in vitro propagation using various explants such as auxiliary buds (Chaturvedi and Sharma 1989, Llorente and Apostolo 1998), nodal explants (Tyagi and Prakash 2004, Hegazi et al 2014) and shoot tips (Sardana and Batra 1998) are available. In vitro rooting percentage ranged from 20 and 95% depending on the clone and the specific trial.…”

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

“…Similar results were reported in Camellia sinensis Linnaeus and Musa acuminata Colla (Mondal et al 1998;Farahani et al 2008). The effect of alone 6-benzylaminopurine (BAP) and in combination with auxin on micropropagation of Jojoba has been documented by different rates of shoot multiplication (Driver, Kuniyuki 1984;Chaturvedi, Sharma 1989;Mills et al 1997;Llorente, Apóstolo 1998;Tyagi, Prakash 2004;Singh et al 2008). In the present study, TDZ alone could give rise to 20 shoots.…”

“…This may be the reason why during the present study TDZ might have scored higher numbers of shoots. BAP is reported to produce a higher number of shoots per explant, however, it induces callus and hyperhydrated shoots (Llorente, Apóstolo 1998, 2000Mills et al 2004;Tyagi, Prakash 2004). We could achieve more than 90% in vitro rooting, which was higher than earlier (Chaturvedi, Sharma 1989;Mills et al 1997;Tyagi, Prakash 2004).…”

“…Vegetative propagation could be used for the generation of selected clones with limited success (Loreto et al 1998). In order to overcome the difficulties in vegetative propagation methods for micropropagation have been developed using an in vitro rooting method (Chaturvedi, Sharma 1989;Mills et al 1997;Llorente, Apóstolo 1998;Roussos et al 1999;Hamama et al 2001;Tyagi, Prakash 2004;Llorente et al 2007;Singh et al 2008). Ex vitro rooting is a promising method, skipping the phase of in vitro rooting, shortens the micropropagation cycle by 2-3 weeks or more and so minimizes the time period of the plantlet generation (Debergh, Maene 1981;Martin 2003).…”