Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

Bekheet, S. A.; Gabr, A. I.; Reda, A. S.; Bahr, MK El

doi:10.3329/ptcb.v25i2.26252

Cited by 16 publications

(15 citation statements)

References 16 publications

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“…By comparing both PEG and sucrose, it could be suggested that PEG has a greater in uence on the growth of G. wallichianum than does sucrose. Similar results have been observed for Lathyrus sativus (Piwowarczyk et al 2014), Milk thistle (Bekheet et al 2016), and four varieties of durum wheat (Bouiamrine and Diouri 2012). According to Razavizadeh et al (2019), adding PEG to the medium induced osmotic stress in cultured Thymus vulgaris explants, and similar conditions were found in all plants subjected to drought stress via a reduction in the fresh weight and length of thyme explants, which was consistent with other studies on several aromatic plants (Bettaieb et al 2009;Lutts et al 2004).…”

Section: Discussionsupporting

confidence: 83%

Exploring the Potential of PEG and Sucrose as Growth Retardant of Geranium wallichianum

Ashraf,

Khan,

Baig

et al. 2023

Preprint

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Geranium wallichianum is a medicinal plant belonging to the Geraniaceae family. It is mainly found in the East Asian Himalayas. Because of its numerous ethnomedicinal uses in the community, this plant is threatened by over-exploitation and over-harvesting. Pakistan has already lost its population by more than 75%. Considering its medicinal importance, this plant must be conserved before it becomes extinct. This study aimed to provide the protocol for in vitro conservation of G. wallichianum. Owing to the plant conservation applications of osmotic stress inducers such as polyethylene glycol (PEG) and sucrose, their ability to regulate the in vitro growth of this medicinally significant plant was evaluated. Using nodal segments as explants, the effect of Murashige and Skoog (MS) medium supplemented with different concentrations of PEG (0, 0.5, and 1.5%) and sucrose (0, 4, and 8%) on various growth parameters was investigated. At 45-day intervals, PEG (0.5 and 1.5%) treatment was found to be more effective than sucrose treatment in reducing in vitro growth (0.92 and 0.57 cm), with minimum fresh (0.06 and 0.04 g) and dry weight (0.04 and 0.02 g), respectively. Total soluble sugars (4.07 and 4.12 mg/g) and total phenolic content (0.96 and 0.19 mg/g) were significantly increased by PEG treatment (0.5 and 1.5%, respectively), whereas total chlorophyll content (0.17 and 0.14 mg/g) and total protein content (2.38 and 2.32 mg/g) were decreased. In addition, Pearson correlation analysis confirmed that total phenolic content was negatively correlated with chlorophyll a, chlorophyll b, and total chlorophyll content, whereas shoot length was negatively correlated with total phenolic content but positively correlated with fresh weight and dry weight. According to the principal component analysis, total phenolics and total sugars, which clustered distinctly from the other parameters, indicated relatively higher osmotic stress responses to PEG and sucrose. Based on these findings, it appears that PEG was more effective than sucrose at slowing in vitro growth during short-term conservation.

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Section: Discussionsupporting

confidence: 83%

Exploring the Potential of PEG and Sucrose as Growth Retardant of Geranium wallichianum

Ashraf,

Khan,

Baig

et al. 2023

Preprint

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“…SCoT markers can be used effectively for both genetic diversity and similarity studies [51, 52]. SCoT markers were already reported for assessing the genetic stability of several plant species such as Cleome gynandra [53], Pittosporum eriocarpum [48] , Albizia julibrissin [54], and Simmondsia chinensis [55]. Similarly, ISSR primer based assessment of regenerans was reported in Tylophora indica [56], Simmondsia chinensis [57], Rauwolfia tetraphylla L. [58], Spilanthes calva [59], Cucumis melo L. [60], Cornus alba [61], and Zea mays [62].…”

Section: Resultsmentioning

confidence: 99%

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Rohela

Jogam

Bylla

et al. 2019

BioMed Research International

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Rauwolfia tetraphylla L. is an important medicinal plant species which is well known for its pharmaceutically important alkaloids. In the present study, we are reporting about its conservation by in vitro clonal multiplication through the standardized protocol of indirect regeneration by using leaf and stem based callus and assessment of genetic fidelity of acclimated plantlets by start codon targeted (SCoT), inter simple sequence repeats (ISSR), and randomly amplified polymorphic DNA (RAPD) marker based analysis. Initially friable callus was induced in maximum amounts (378.7, 323.8, and 412.8 in mg) from leaf, root, and stem explants on Murashige and Skoog (MS) media supplemented with 5.0 mg/L, 3.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) and 5.0 mg/L of naphthalene acetic acid (NAA), respectively. Shoot regeneration with the maximum number of shoot buds (25 and 20) was obtained from leaf and stem calluses on MS media supplemented with TDZ (0.25 mg/L) + BAP (2 mg/L). The regenerated shoots were rooted successfully with maximum rooting percentage of 98.0 on full strength MS media amended with IAA (1.0 mg/L) and IBA (1.0 mg/L). The regenerated plantlets were hardened using 2:1 ratio of sterile garden soil and sand, followed by acclimatization in field conditions with 86% of survival. SCoT, ISSR, and RAPD primers based polymerase chain reaction (PCR) analysis was carried out to check possible genetic variations in micro propagated plants in comparison with mother plant. Among the ten SCoT (S), ISSR (R), and RAPD (OPA) primers used, S2, R10, and OPA3 has given good amplification with scorable DNA bands. The results revealed that the regenerated plants did not have any polymorphism with mother plant. Hence, the in vitro regenerated R. tetraphylla plantlets were confirmed as true-to-type.

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“…Micro propagation offers opportunities for production of elite and true to type plants from the selected stock plant of jojoba in a short time independent of the season 4 . In this respect, considerable differences among clones in response to growth regulators at all stages of in vitro propagation have been reported.…”

Section: Introductionmentioning

confidence: 99%

Zeatin and Thidiazuron Induced Embryogenic Calli From In Vitro Leaf and Stem of Jojoba (Simmondsia chinensis)

El-Ashry

Gabr

Bekheet

2017

Pakistan J. of Biological Sciences

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Background and Objective: Jojoba is a promising industrial plant, which recommended with pharmaceutical benefits. The present study was conducted to stimulate embryogenic calli formation from jojoba using zeatin and thidiazuron (TDZ), as well as determination of the antioxidant activity of proliferated calli. Materials and Methods: For callus induction, leaf and stem explants derived from in vitro grown shootlets, were cultured on Murashige and Skoog (MS) medium with different combinations of 0.5 mg LG 1 benzyl adenine (BA) or kinetin with 2,4-Dichlorophenoxyacetic acid (2,4-D), Naphthalene acetic acid (NAA) and picloram at 0.5 or 1mg LG 1 . To stimulate embryogenic calli, friable callus were transferred to woody plant medium (WPM) supplemented with different concentrations of zeatin or TDZ. Antioxidant activity of different treatments was determined using hexane or petroleum ether extraction. Data was analyzed as mean±standard deviation (SD). Results: The MS medium supplemented with 0.5 mg LG 1 BA+0.5 or 1 mg LG 1 picloram was the best treatment to obtain friable calli from both explants types. WPM medium supplemented with 2 mg LG 1 zeatin gave the highest percentage of embryogenic calli derived from leaf explants. While the highest percentage of embryogenic calli derived from stem explants was registered using 1 or 4 mg LG 1 TDZ containing medium. Embryogenic calli originated from leaves explants on 1.5 mg LG 1 zeatin showed promising activity of antioxidant with hexane extraction. However, embryogenic calli originated from stem explants on 1 mg LG 1 TDZ showed the highest antioxidant activity with petroleum ether extraction. Conclusion: TDZ has promising effect on embryogenic callus induction from stem explants. While, zeatin has promising effect on embryogenic callus induction from leaf explants.

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Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

Cited by 16 publications

References 16 publications

Exploring the Potential of PEG and Sucrose as Growth Retardant of Geranium wallichianum

Exploring the Potential of PEG and Sucrose as Growth Retardant of Geranium wallichianum

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Zeatin and Thidiazuron Induced Embryogenic Calli From In Vitro Leaf and Stem of Jojoba (Simmondsia chinensis)

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