Effect of different extraction techniques on quantification of oleanolic and ursolic acid in Lamii albi flos

Wójciak–Kosior, Magdalena; Sowa, Ireneusz; Kocjan, Ryszard; Nowak, Renata

doi:10.1016/j.indcrop.2012.11.018

Cited by 55 publications

(45 citation statements)

References 42 publications

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“…Quantification of betulinic acid (BA) in the extracts was performed by HPLC Agilent 1200 series from Agilent Technologies Inc. (Santa Clara, California, USA) according to a method previously described [19] with some modifications. Briefly, separation was carried out using a C-18 reverse phase column (250 x 4.6 mm, 5 μm), version B.04.03.…”

Section: Discussionmentioning

confidence: 99%

“…The use of UAE to recover triterpenic acids from different plant matrix has been recently studied [18][19][20] and has proved to present several advantages in comparison with conventional solid-liquid extraction. These advantages include reduction of the amount of solvent required, time and temperature, which represents an important factor when extracting thermolabile compounds [21].…”

Section: Introductionmentioning

confidence: 99%

“…Also PLE to recover triterpenic acids from different botanical sources has been previously reported [19,23]. PLE uses high pressures in order to remain solvents in liquid state beyond their normal boiling point.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recovery of betulinic acid from plane tree (Platanus acerifolia L.)

Pinilla

López-Padilla

Vicente

et al. 2014

The Journal of Supercritical Fluids

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Betulinic acid (3β, hydroxy-lup-20(29)-en-28-oic acid) is a bioactive triterpenic acid which was identified in various botanical sources and in considerable amounts in the bark of plane tree (Platanus acerifolia L.). In this work, the recovery of betulinic acid from plane tree bark was studied using different liquid solvent based extraction methods, namely Solid-Liquid Extraction (SLE), Ultrasound Assisted Extraction (UAE) and Pressurized Liquid Extraction (PLE). Furthermore, preliminary studies of the Supercritical Fluid Extraction (SFE) of plane tree bark are also reported.The liquid solvent based extraction techniques (SLE, UAE and PLE) were carried out using ethanol and ethyl acetate, and produced a recovery of betulinic acid in the range 10-15 mg per g of bark, with concentrations around 25-35 % mass. A betulinic acid enrichment in the ethanolic extracts was possible by means of a simple precipitation step adding water. The precipitate contained 42-46 % mass of betulinic acid and high 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 recovery (> 95%). Increasing the extraction temperature, by means of the PLE assays, has not resulted in an improvement of betulinic acid recovery. Manuscript Click here to view linked ReferencesThe preliminary SFE assays produced lower recoveries of betulinic acid (0.5-8 mg/g) with respect to liquid extraction. The addition of ethanol as cosolvent produced a significant improvement of both betulinic acid recovery and concentration in the SFE extract.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Recovery of betulinic acid from plane tree (Platanus acerifolia L.)

Pinilla

López-Padilla

Vicente

et al. 2014

The Journal of Supercritical Fluids

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“…In recent years they became the subject of many publications because of their various activities combined with low toxicity. 2 Eucalyptus spp. (family Myrtaceae) originated in Australia, but these plants now grow in almost all tropical and sub-tropical areas and are cultivated in many other climates.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Bioactive Triterpene acids (Ursolic acid and Oleanolic acid) in Different Extracts of Eucalyptus globulus (L) by HPTLC Method

Gupta¹,

Maheta²,

Chauhan³

et al. 2017

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Objective: To develop a novel analytical method for simultaneous determination of two triterpenic acids by high-performance thin layer chromatography in methanol and dichloromethane extracts of Eucalyptus globulus leaf. Ursolic acid was also isolated from Eucalyptus globulus leaf. Materials and Methods: Two triterpenic acids (ursolic and oleanolic acid) were extracted using methanol and dichloromethane as the extraction solvents. Study for total triterpenoids present in Eucalyptus globulus leaves was carried out which shows considerable amount of terpenoids present. Because of the similarity of chemical structure, the prechromatographic derivatization was necessary to separate these triterpenic acids. The samples were treated by 1% iodine solution in chloroform directly on the chromatographic plate and developed with the mobile phase consisting of petroleum ether, ethyl acetate and acetone (7.8:2.2:0.1, v/v/v). After drying, the plates were sprayed with 10% (v/v) ethanol solution of sulfuric acid and heated to 120 °C for 3 min. Quantification was performed in absorbance/transmittance mode at a wavelength of 345 nm. The developed HPTLC method was validated for linearity, precision and accuracy.

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“…Different techniques have been employed for the extraction of OA such as heat reflux, hydrotropic extraction, microwave assisted extraction, and supercritical fluid extraction [11] [12]. Heat reflux extraction and maceration extraction techniques have drawbacks of requiring longer extraction time and relatively large volumes of solvents.…”

Section: Introductionmentioning

confidence: 99%

Ionic Liquid Based Ultrasonic-Assisted Extraction of Oleanolic Acid from Grape Seeds

Zhang¹,

Li²,

Qian³

et al. 2017

OALib

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Ionic liquid based ultrasonic-assisted extraction (ILUAE) was successfully applied to extract Oleanolic acid (OA) from grape seeds. Different kinds of 1-alkyl-3-methylimidazolium ionic liquids with different kinds of cations and anions were studied and 1-butyl-3-methylimidazolium chloride ([C 4 mim]Cl) solution was selected as extractants. The optimal conditions of ILUAE were determined by single factor experiments and Box-Behnken design as follows: [C 4 mim]Cl concentration of 0.7 mol/L, extraction time of 4 h, liquid-solid ratio of 15:1 mL/g, ultrasonic power of 195 W, the ultrasonication time of 13 min, and extraction temperature of 48˚C·min. The extraction yield of OA was 13.4 mg/g, which is well in close agreement with the value predicted by the model. Compared with the regular ultrasonic-assisted extraction (UAE), the proposed approach exhibited 1.64 times higher efficiency, which indicated that ILUAE has a broad prospect for sample preparation of the bioactive compounds.

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Effect of different extraction techniques on quantification of oleanolic and ursolic acid in Lamii albi flos

Cited by 55 publications

References 42 publications

Recovery of betulinic acid from plane tree (Platanus acerifolia L.)

Recovery of betulinic acid from plane tree (Platanus acerifolia L.)

Simultaneous Quantification of Bioactive Triterpene acids (Ursolic acid and Oleanolic acid) in Different Extracts of Eucalyptus globulus (L) by HPTLC Method

Ionic Liquid Based Ultrasonic-Assisted Extraction of Oleanolic Acid from Grape Seeds

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