Objective: To develop a novel analytical method for simultaneous determination of two triterpenic acids by high-performance thin layer chromatography in methanol and dichloromethane extracts of Eucalyptus globulus leaf. Ursolic acid was also isolated from Eucalyptus globulus leaf. Materials and Methods: Two triterpenic acids (ursolic and oleanolic acid) were extracted using methanol and dichloromethane as the extraction solvents. Study for total triterpenoids present in Eucalyptus globulus leaves was carried out which shows considerable amount of terpenoids present. Because of the similarity of chemical structure, the prechromatographic derivatization was necessary to separate these triterpenic acids. The samples were treated by 1% iodine solution in chloroform directly on the chromatographic plate and developed with the mobile phase consisting of petroleum ether, ethyl acetate and acetone (7.8:2.2:0.1, v/v/v). After drying, the plates were sprayed with 10% (v/v) ethanol solution of sulfuric acid and heated to 120 °C for 3 min. Quantification was performed in absorbance/transmittance mode at a wavelength of 345 nm. The developed HPTLC method was validated for linearity, precision and accuracy.
Among the loose flowers the jasmine is very important and remunerative crop. Multiuse of these flowers like used as loose flowers as well as for extraction of essential oil makes it more remunerative. The Indian J. grandiflorum compared favorably with that of Spanish jasmine, both in yield and quality of oil. The flower is in high demand in places such as Mumbai, besides the coastal region. Local traders collect jasmine flowers from the farmers directly. Jasmine begins to flower in the second year onwards or sometimes even earlier, but economic yields are obtained only from the third year. Prices are decided at the auction centre. Poor women in other cities across India can adopt jasmine cultivation as an activity to earn income and eradicate poverty. Moreover, this activity takes place early morning and the women are free to undertake other work during the day. Though the jasmine is a high capital intensive crop but its cultivation is quite profitable. The investment in jasmine is economically feasible and financially sound. Jasmine is labour-intensive crop, it provide more man days labour per year.
The present experiment entitled "Effect of pre-plant soaking of corms in bio-regulators on growth, flowering, yield and quality in gladiolus (Gladiolus grandiflorus L.) cv. Rani." was carried out at the Jambuvadi Farm, Department of Horticulture, J. A.U., Junagadh (Gujarat) during 2019 to 2020. The results of the study indicated that, the pre-plant soaking of corms in bio-regulator GA3 (150 ppm) was found effective for earlier sprouting (6.90 days), maximum plant height (78.74 cm) and number of leaves (13.62). Regarding flowering characters, the GA3 150 ppm responded best especially in commercial traits like spike length (86.82 cm), rachis length (40.30 cm), number of floret/spike (10.63), diameter of 2 nd floret (13.09 cm) and duration of flowering (22.34 days).
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