Effect of different extraction and precipitation methods on yield and quality of pectin

Abstract

An experimental study has been conducted to determine the combined effects of different extraction conditions and precipitation method on the yield and quality of high methoxyl pectin from lemon peels. Pectin was extracted using different mineral acids (HCl, HNO3 and H2SO4) at four concentration levels (0.025; 0.05; 0.1 and 0.2 m), at 70 °C for 4 h. The soluble pectin was precipitated by iso-propanol or by an aluminium sulphate, Al 2(SO 4) 3, solution at pH 4. The extraction with HCl and HNO 3, at the highest concentrations investigated, followed by aluminium precipitation led to the best results in terms of yield (22-25%), quality and gelling power of pectin with a remarkable decrease of alcohol consumption as compared to the alcoholic precipitation under the same extracting conditions

Extracción Rápida de Pectina a Partir de Cáscara de Maracuyá (Passiflora edulis flavicarpa) empleando Microondas

“…La técnica de extracción asistida por microondas se basa en el uso de la energía de microondas para conseguir que los compuestos de interés pasen rápidamente de la muestra a un disolvente adecuado, además, es una técnica que utiliza volúmenes pequeños de solvente y permite el control de una serie de parámetros que afectan a la eficacia de extracción (Seixas et al, 2014). Para el caso de la extracción de pectina de la cáscara de maracuyá se han realizado investigaciones con los métodos convencionales e hidrólisis ácida, obteniéndose rendimientos de extracción entre 10 y el 70% (Kliemann et al, 2009;Liew et al, 2014;Freitas de Oliveira et al, 2016), estudios realizados por Kalapathy y Proctor (2000) y Seggiani et al, (2009) indican que la extracción con HCl se obtienen mayores rendimientos y mejor calidad de pectina y menor consumo de alcohol en la precipitación y Aunque existen reportes de extracción de pectina de la cáscara de maracuyá asistida por microondas, estos no tienen en cuenta la variación de la concentración del ácido utilizado, el cual ofrece un gran aporte en la hidrólisis de la protopectina y exposición de la pectina al solvente (Seixas et al, 2014).…”

Influence of different treatment condition on biopolymer yield production for coagulation-flocculation process

“…The mixture was then extracted in a water bath stirrer at 80°C/ 90 o C for 0.5/ 4 h, while being stirred. The mixture was left to cool to room temperature before the pH of mixture was increased to pH 3.5 by adding KOH 0.2M to promote pectin extraction [11]. Next it was filtered through 125 mm Whatman No.1 filter paper.…”

Effect of water addition on pectin recovery from solution in centrifugal separation process

“…Different extraction procedures have been proposed on laboratory scale, depending upon the type of pectin source and expected characteristics of the final product (Sun & Hughes, 1998; Yeoh et al. , 2008; Seggiani et al. , 2009).…”

“…In industrial applications, before the separation of pectin solution, the pH is increased above three to promote pectin extraction (Seggiani et al. , 2009) and to stabilise the slurry against further hydrolysis (Yapo, 2009).…”

“…Common steps of the production process are (May, 1990) extraction of pectin by hydrolysing the organic material (usually using mineral acid), separation of pectin solution from the resulting slurry and pectin precipitation and purification. Different extraction procedures have been proposed on laboratory scale, depending upon the type of pectin source and expected characteristics of the final product (Sun & Hughes, 1998;Yeoh et al, 2008;Seggiani et al, 2009).…”