Effect of different extenders on ram sperm traits during storage

Abstract

The aim of the study was to test commercial extenders used for short-term and long-term sperm preservation. Semen was collected in the reproduction season, i.e. from June to December. The ejaculates were obtained from single services and the routine analysis of the semen was performed immediately after the collection. The examination included semen volume, colour and texture, sperm concentration and motility, ejaculate turbulence and percentage of sperm with abnormal morphology. The semen was diluted with an extender in the ratio of 1:4. The processed semen was transported in an insulated container at 16–18 °C to the laboratory and stored in a stationary thermostat under the same temperature. Sperm motility tests were performed 24, 48, 72 and 96 hours after the placement in to thermostat. Ejaculates diluted with Ovipro, Optidyl, Triladyl and Andromed CSS gave very good results of viability (81.23 %–83.41 %) after 24 hours of storage. After 48 hours, Ovipro, Andromed, Optidyl and Triladyl gave values above 75 %. The Triladyl extender proved to be a good stabilizing agent, showing consistent results during a long-term storage. It was chosen as a control one for overall assessment. Other preservation media did not show any improving or worsening effects. The extender Ovipro showed a high motility effect in the first 48 hours only, and hence it appears to be the best solution for the short-term preservation.

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

“…Sperm viability after storage at 4 o C varied with extender in ram (Rakha et al, 2013). Previous studies observed that Triladyl showed better sperm viability after 24 h (81.2%) of storage at 4 o C compared with semen stored in other extenders (78.6% for Andromed or 77.9% for Biladyl; Hegedűšova et al, 2012). In order to get a satisfactory conception rate, cold semen should have >70% viable spermatozoa in bovine (Nilani et al, 2012).…”

“…The most common semen extenders for sheep are based on Tris and egg yolk. There were studies conducted to compare ram semen quality after chilling diluted with commercial extenders (Kasimanickam et al, 2011;Hegedűšova et al, 2012). However, there are no reports regarding chilled preservation of indigenous ram semen with commercial specially Triladyl and tris fructose egg yolk (TFE) extenders and their effects on PR in Indigenous ewes in Bangladesh.…”

Effect of Supplementation of Glutathione and α-Tocopherol in Tris and Skim Milk Based Extenders on Motility Parameters of Ram Semen at Refrigeration Temperature

“…Many kind of extenders like TRIS-egg yolk, skim milk, sodium citrate-egg yolk, coconut milk etc. are used in addition to many other traditional extenders successfully in preserving ram semen (Hegedusova et al, 2012). El-Gaafary (1987) suggested that skim milk extender was found to be a good medium for preserving the fertilizing capacity of ram spermatozoa for three h at 5ºC during storage.…”