Comparison of commercial Triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in Bangladeshi indigenous sheep (Ovis aries)

Rekha, Azizunnesa; Zohara, Begum Fatema; Bari, Farida Yeasmin; Alam, Golam Shahi

doi:10.1016/j.smallrumres.2015.12.007

Cited by 15 publications

(16 citation statements)

References 21 publications

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“…Sheep insemination pipette with an excentrical tip (Minitube, Germany) with 0.25 ml semen straw was introduced in to the cervix and tried to push forward by manipulating through the cervical rings. Semen was then expelled from the pipette as deeply as possible in the cervix (Kumar and Naqvi, 2014;Rekha et al, 2016).…”

Section: Transcervical Ai In Indigenous Ewesmentioning

confidence: 99%

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

Rekha¹,

Zohara²,

Bari³

et al. 2016

Anim Reprod

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Two different extenders were compared for their effects on preservation of semen from Indigenous rams and on pregnancy rate (PR) in Indigenous ewes. Semen was collected from nine Indigenous rams (Ovis aries) once a week using an artificial vagina. Each ejaculate was divided into 2 aliquots, diluted with either commercial (Triladyl ® ) or locally manufactured (tris, fructose, citric acid, egg yolk: TFE, prepared in own laboratory) extenders and kept at 4°C for 48 h. Motility, viability, functional integrity and morphological changes were evaluated at 0, 24 and 48 h. Synchronized oestrus ewes inseminated transcervically with 24 and 48 h of preserved chilled semen diluted with Triladyl and TFE extenders separately. Semen preserved in Triladyl had better motility, viability, and functional integrity at 24 and 48 h (P < 0.001) than did in TFE. The morphologically normal spermatozoa up to 48 h of preservation did not differ between extenders. However, in abnormalities studied, Triladyl had detrimental effect on sperm acrosome and TFE on sperm tail (P < 0.001) at 24 and 48 h of preservation. But, midpiece was not affected by any extender (P > 0.05) over the entire period of preservation. The quality of semen decreased (P < 0.001) with increasing preservation time for both extenders. The extenders did not differ (P > 0.05) the overall PR after transcervical AI (TCAI) in ewes. Increased preservation time (48 h) negatively affected the PR in TFE extended semen compared with than that of Triladyl. The results suggest that the quality of chilled semen (motility, viability, and functional integrity) is more improved when preserved in Triladyl than if extended with a TFE. PR may higher when TCAI is performed with chilled semen preserved in Triladyl for a longer time than TFE. However, TFE extender may be used to dilute the semen for chilling and used in TCAI to get similar PR of Triladyl up to 24 h of preservation.

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Section: Transcervical Ai In Indigenous Ewesmentioning

confidence: 99%

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

Rekha¹,

Zohara²,

Bari³

et al. 2016

Anim Reprod

Self Cite

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“…Functional membrane integrity (FMI). FMI was evaluated by the hypoosmotic test (HOST) (Rekha et al, 2016). For this, 50 μL of thawed semen was mixed with 200 μL of a prewarmed (37 °C) hypoosmotic solution of 5.4% sucrose (100 mOsmol/l) in Falcon tubes, and incubated at 37 °C for minutes.…”

Section: Structural Membrane Integrity (Smi)mentioning

confidence: 99%

Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen

Varela-Giraldo

Úsuga-Suárez

Duque-Cortes

et al. 2020

Rev. colomb. cienc. pecu.

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“…Usually, during freezing-thawing process of semen, the sperm undergoes several vital biological and functional changes due to cold shocks and cryo-injuries, especially affects membranes that distresses their functional abilities (Nur et al, 2010;Chelucci et al, 2015). To establish a simple and sustainable cryopreservation protocol for ram semen in Bangladesh, efforts are focused on different types of diluents, dilution and freezing methods (Mahmuda et al, 2015;Rekha et al, 2016;Rekha et al, 2018;Jha et al, 2019). Evidence from previous studies, a hand-made tris-fructose-citrate (20% egg yolk and 5-7% glycerol) and commercial diluents Triladyl ® , two-steps dilution (fractional procedure) and three-steps manual freezing (in LN2 vapourusing styrofoam) are seemed to be recommended protocol for freezing ram semen in Bangladesh, already extended for field fertility testing (Rekha et al 2016;Rekha et al 2018;Jha et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…To establish a simple and sustainable cryopreservation protocol for ram semen in Bangladesh, efforts are focused on different types of diluents, dilution and freezing methods (Mahmuda et al, 2015;Rekha et al, 2016;Rekha et al, 2018;Jha et al, 2019). Evidence from previous studies, a hand-made tris-fructose-citrate (20% egg yolk and 5-7% glycerol) and commercial diluents Triladyl ® , two-steps dilution (fractional procedure) and three-steps manual freezing (in LN2 vapourusing styrofoam) are seemed to be recommended protocol for freezing ram semen in Bangladesh, already extended for field fertility testing (Rekha et al 2016;Rekha et al 2018;Jha et al, 2019). However, more effort needs to standardize and select a sustainable frozen ram semen production technology, both in terms of dilutes and freezing methods.…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Freezing Methods and Diluents Types on Post-Thaw Sperm Quality of Ram Sperm

Jha¹,

Alam²,

Bari³

2020

Res. Agric. Livest. Fish.

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The effect of freezing methods and diluents types on post-thaw sperm quality of Bangladeshi ram semen was studied. Two freezing methods and three diluents was tested as pooling effects (freezing methods or diluents) on post-thaw sperm parameters; sperm motility (SM), viability (SV), plasma membrane integrity (SPMI) and acrosome integrity (SAI), respectively. From selected ten rams, eight ejaculates were used for each freezing group (freezing methods × diluents). Semen samples were diluted by using two-steps for hand-made tris-based diluents (20% egg yolk): D1 (7% glycerol) and D2 (5% glycerol), and one-step dilution for commercial diluents: D3 (Triladyl®) at 35°C. After 4h of equilibration of temperature at 5°C, diluted semen samples was aspirated into 0.25 mL straws, and sealed. Straws were frozen in liquid nitrogen (LN2) vapour using two methods: F1 (manually in Styrofoam box, using three-steps method; +5°C to -80°C at -11.33°C/min, -80°C to -120°C at -26.66°C/min, and -120°C to -140°C at - 13.33°C/min) and F2 (programmable bio-freezer, using two-steps method; +5°C to -100°C at - 20°C/min and -100°C to -140°C at -10°C/min). Two semen straws from each batch were evaluated (37°C for 20 sec) for sperm parameters. In pool effects between freezing methods; SAI differed significantly (P < 0.001). The SM (56%) and SV (72%) were observed competitive. However, SPMI (67.58 ± 2.02%) and SAI (76.13 ± 1.42%) were higher in F1. Among diluents, SM (P < 0.006), SV (P < 0.008), SPMI (P < 0.012) and SAI (P < 0.019) differed significantly. The SM (61.25 ± 1.80%), SV (77.13 ± 1.47%), SPMI (68.31 ± 1.91%) and SAI (74.75 ± 1.64%) were highest in D3. In conclusion, the combination of manual freezing (three-steps) and handmade tris-based diluents (20% egg yolk, 5% glycerol) is suitable and sustainable method for cryopreservation of ram semen. Res. Agric., Livest. Fish.7(2): 235-241, August 2020

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Comparison of commercial Triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in Bangladeshi indigenous sheep (Ovis aries)

Cited by 15 publications

References 21 publications

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen

A Comparison of Freezing Methods and Diluents Types on Post-Thaw Sperm Quality of Ram Sperm

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