EFFECT OF DIFFERENT DOSES OF SYNTHETIC HORMONE LHRH-A2 ON SERUM SEX HORMONES, OVULATION PERCENT AND EGG HATCHING RATES OF PERSIAN STURGEON Acipenser persicus

Abstract

Acipenser persicus is one of the economically valuable fishes in the Caspian Sea. Due to the economical and cultural importance of sturgeons in the world, understanding of sturgeon reproduction is necessary for successful management of their populations in aquaculture and nature. To improve the culture methods and to facilitate captive breeding programs to support restocking, it is necessary to understand the changes in steroid hormones during artificial reproduction. The administration of sturgeon luteinizing hormone -releasing hormone analogue (LHRH-A 2 ) induces changes in serum sex steroid levels. To study the effects of different doses of LHRH-A 2 hormone on induction of final maturation and ovulation of Persian sturgeon, 18 maturating females and 18 maturating males were used. All brooders were injected with LHRH-A 2 in dosages of 4, 6 and 8 µg kg -1 . In this study, LHRH-A 2 successfully induced final maturation and ovulation in females, and the highest percentage of ovulated females was observed in the groups treated with dose of 4 µg kg -1 LHRH-A 2 . There were significant differences in serum cortisol (C), testosterone (T) and Gonadotropin hormone (GTHII) concentrations at tested dosages. In hormonal treatment groups, the highest hatching rate was observed in the groups receiving 4 µg kg -1 LHRH-A 2 . Finally, the LHRH-A 2 in dose of 4 µg kg -1 is effective in stimulating oocyte maturation and ovulation in Persian sturgeon. How to CiteMohammadi, H., Khara, H., Kazemi, R. (2015): Effect of different doses of synthetic hormone LHRH-A 2 on serum sex hormones, ovulation percent and egg hatching rates of Persian sturgeon Acipenser persicus. Croatian Journal of Fisheries, 73,[58][59][60][61][62]

Spawning induction in Sterlet sturgeon (Acipenser ruthenus) with recombinant GnRH: Analysis of hormone profiles and spawning indices

“…In the current study, a positive effect of rGnRH treatment was displayed on the number of eggs per gram. It was consistent to previous research on Persian sturgeon, showing that increasing the LHRH-A2 levels from 4 to 6 µg kg -1 BW also increased the number of eggs per gram (Mohammadi et al, 2015). The egg quality and quantity from induced spawning appear to be partially related to the interaction between the stage of maturity or oocyte diameter and GnRHa dose (Ibarra and Duncan, 2007).…”

Dynamics of the content of H2O, Na, K, Ca and Mg in the eggs of bream, Abramis brama L. in natural conditions and under stress

“…Nowadays, the methods of hypophysial and hormonal injections are widely used in fish-breeding for stimulation of oocytes maturing and ovulation process [63][64][65][66][67][68][69]. It is shown that the result depends on conditions of incubation and application of hormonal preparations [61,62].…”

EFFECT OF WONDERFUL KOLA SEED MEAL (Buccholzia coriacea) ON GROWTH, MASCULINIZING POTENCY AND GONAD GROSS MORPHOLOGY OF THE NILE TILAPIA (Oreochromis niloticus, LINNAEUS 1758)

“…This strategy was first developed in Japan in the 1950s for reversing the sex of aquarium fish and carp species and commercialized in the 1970s (Baroiller and D'Cotta, 2000). In fish, control of reproduction requires gametes manipulation (Mohammadi et al, 2015).…”