Effect of Different Doses of Doxorubicin on Pituitary Gland and Some Testicular Function in Adult Male Rabbits

Abstract

The present study was designed to search out the effect of different doses of doxorubicin some functions of pituitary gland and testes in adult male rabbits. Twenty adult male rabbits were randomly divided into four equal groups and treated for 28 days as follows: first group (control) were injected with normal saline; second group (GI) were injected with doxorubicin 3 mg/ kg B.W., while animals in the third group (GII) and fourth group (GIII) injected with 3.5 and 4 mg/ kg body weight of doxorubicin respectively, all animals were injected twice a week via ear vein. At the end of experiment fasting blood (8-10 hrs) samples were collected. Blood was drawn by cardiac puncture technique and serum was collected for measuring the hormones, Follicular Stimulating Hormone (FSH), Luteinizing Hormone (LH), and Testosterone (T). In addition, sections from pituitary gland and testes were taken for histopathological studies. The results showed significant increase (P<0.05) in serum FSH, LH and T levels in group (GIII) as compared to GI, GI and control groups. Beside significant increases in T levels was observed in GII as compared to control group. Sever histopathological changes was observed in testes including thickness of basement membrane, protienous material in lumen of somniferous tubules and pituitary gland showed fibrosis, faculation of epithelial cell in all treated groups. In conclusion different doses of doxorubicin have detrimental effect on pituitary gland and male reproductive system of rabbits

Electrocardiographically study of protective effect of Eruca Sativa M. versus doxorubicin induced cardiotoxicity in rabbits

“…Thirty-five adult rabbits (n=35) will randomly divided into four group (7 rabbits/each group) as following: (A) first group: seven rabbits will received distal water (D.W) orally (-ve control), (B) Second group: fourteen rabbits divided to two subgroup equally administrated with doxorubicin 4 mg/kg B.W (Al-okaily et al,2013) (+ve control) intraperitoneally (I.P) between day to day for 14 days to induce cardio toxicity (Swamy et al, 2011) (first subgroup leave two weeks then killed, while second subgroup leave to 28 days without treatment), (C) Third group: seven rabbits administrated with Eruca sativa mill (gergeer) Leaves ethanolic extract daily for 28 days Orally by stainless gavage needle, (D) Fourth group: seven rabbits administrated with Eruca sativa(gergeer) Leaves ethanolic extract orally daily for 14 days as protective then we give doxorubicine 4 mg/kg B.W (Al-okaily et al, 2013) intraperitoneally (I.P) between day to day for 14 days (Swamy et al, 2011). While continuing to give Eruca sativa mill (Gergeer) orally for 28 days.…”

LD50 and affective dose of Eruca sativa mill (gergeer) ethanolic extract

“…The pilot study of Eruca Sativa Mill in rabbits as in ( The calculated value of LD50 of Eruca sativa in the rabbit was estimated by "up and down" method, the result of rabbit were shown in table (1)(2)(3) for each group. The Troponin-I level of the control positive group revealed a significant increase (P<0.05) as compare with G3,G4,G5 and control negative group (Dox) with no significant variance (P<0.05) in comparison with G1 at dose (25 mg/kg B.W) and G2 at dose (50 mg/kg).…”

“…The cTn-I level of the G3 at dose (100 mg/kg B.W) exhibited a significant elevation (P>0.05) is compared with treated G4,G5 group at a dose (200,400 mg/kg B.W) and control negative group with a significant reduction (P<0.05) is compared with G1, G2 group and control positive group ; while treated G4 ,G5 group at dose(200 ,400 mg/kg B.W) exhibited a significant decline (P>0.05) is compared with treated G1,G2,G3 group and control positive at a dose (25,50,100 mg/kg B.W) and there was no statistical different (P>0.05) when compared with control negative group. As in table (1)(2)(3)(4).…”