Effect of different culture systems on adipocyte differentiation-related protein (ADRP) in bovine embryos

Darwich, Abdulrahman Al; Perreau, C.; Tsikis, Guillaume; Coudert, Edouard; Touzé, Jean-Luc; Briant, Eric; Beckers, J. F.; Mermillod, Pascal; Guignot, Florence

doi:10.1016/j.anireprosci.2014.01.010

Cited by 9 publications

(6 citation statements)

References 54 publications

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“…Effects of serum on expression have already been described for other genes, such as related to apoptosis, oxidative stress [ 56 ], embryo quality, gap junctions [ 57 ] and also including those that control cGMP levels [ 17 ]. The increase in PLIN2 transcripts caused by serum had already been reported in cumulus cells [ 17 , 22 , 58 ] and bovine embryos [ 32 ]. These reports and the present study reflect a metabolic response to the enriched media commonly used in in vitro cell culture systems, favoring the accumulation of lipids.…”

Section: Discussionmentioning

confidence: 77%

“…PLIN1, PLIN2 and PLIN3 are members of the PAT family of proteins [ 27 ], which controls the interaction between lipases and LDs; indeed, PLIN2 has been shown to be associated with lipid accumulation [ 28 ]. PLIN2 has been identified in mouse [ 29 ], porcine [ 30 ] and bovine oocytes [ 31 ] and bovine embryos [ 32 ]. In these studies, PLIN2 was shown to co-localize with LDs, indicating its role as a major LD protein in these cells.…”

Section: Introductionmentioning

confidence: 99%

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The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation

et al. 2018

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This study aimed to determine the influence of cyclic guanosine 3’5’-monophosphate (cGMP) and cGMP-dependent kinase (PKG) during in vitro maturation (IVM) on lipolysis-related parameters in bovine cumulus-oocyte complexes (COCs), and on embryo development and cryosurvival. COCs were matured with cGMP/PKG modulators and assessed for metaphase II rates (MII), cGMP levels, lipid content in oocytes (OO), transcript abundance for genes involved in lipolysis (ATGL) and lipid droplets (PLIN2) in cumulus cells (CC) and OO, and presence of phosphorylated (active) hormone sensitive lipase (HSLser563) in OO. Embryo development, lipid contents and survival to vitrification were also assessed. Phosphodiesterase 5 inhibition (PDE5; cGMP-hydrolyzing enzyme) with 10-5M sildenafil (SDF) during 24 h IVM increased cGMP in COCs (56.9 vs 9.5 fMol/COC in untreated controls, p<0.05) and did not affect on maturation rate (84.3±6.4% MII). Fetal calf serum (FCS) in IVM medium decreased cGMP in COCs compared to bovine serum albumin (BSA) + SDF (19.6 vs 66.5 fMol/COC, respectively, p<0.05). FCS increased lipid content in OO (40.1 FI, p<0.05) compared to BSA (34.6 FI), while SDF decreased (29.8 and 29.6 FI, with BSA or FCS, respectively p<0.05). PKG inhibitor (KT5823) reversed this effect (38.9 FI, p<0.05). ATGL and PLIN2 transcripts were detected in CC and OO, but were affected by cGMP and PKG only in CC. HSLser563 was detected in OO matured with or without modulators. Reduced lipid content in embryos were observed only when SDF was added during IVM and IVC (27.6 FI) compared to its use in either or none of the culture periods (34.2 FI, p<0.05). Survival to vitrification was unaffected by SDF. In conclusion, cGMP and PKG are involved in lipolysis in OO and possibly in CC and embryos; serum negatively affects this pathway, contributing to lipid accumulation, and cGMP modulation may reduce lipid contents in oocytes and embryos, but without improving embryo cryotolerance.

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Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation

et al. 2018

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“…Staining and confocal microscopy for further analysis of the embryos were applied to pf day 8 blastocysts after treatment with 0.1 µg mL -1 PFNA during maturation in vitro, a method previously used to detect deviations during early embryo development [38,39]. Images taken as single channel images were converted to overlay images and saved in TIFF format.…”

Section: Staining and Image Analysismentioning

confidence: 99%

Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation

Hallberg

Kjellgren

Persson

et al. 2019

Reproductive Toxicology

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Perfluorononanoic acid (PFNA) is one of the perfluoroalkyl acids present in human tissues. In this study, effects on early embryo development after PFNA exposure were investigated using the bovine in vitro production system. Oocytes were exposed to PFNA during maturation in vitro (10 µg mL-1 and 0.1 µg mL-1), and then fertilized and cultured in parallel with control groups. Developmental parameters (cleavage, blastocyst formation) were followed and embryo quality evaluated (stage, grade). Embryos developed after exposure to 0.1 µg mL-1 were stained to distinguish nuclei, active mitochondria and neutral lipids. 10 µg mL-1 of PFNA had a severe negative effect on blastocyst formation (OR: 0.27 p<0.05), an effect not observed at 0.1 µg mL-1. However, lipid droplet distribution was significantly altered in embryos exposed to 0.1 µg mL-1 , suggesting a disturbance of lipid metabolism after exposure to sublethal levels of PFNA during oocyte maturation in vitro.

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“…Various embryo culture methods have been studied to improve the system, especially in changing the in vitro culture system (Al Darwich et al. ), including adding lipid metabolic regulators (Ruiz et al. ; Stinshoff et al.…”

Section: Introductionmentioning

confidence: 99%

Laminin‐111 Inhibits Bovine Fertilization but Improves Embryonic Development in vitro, and Receptor Integrin‐β1 is Involved in Sperm–Oocyte Binding

Lin

Huang

et al. 2016

Reprod Domestic Animals

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This study detected the distribution of laminin during embryonic formation by immunofluorescence. To determine the possible function of laminin on developmental ability of in vitro fertilized embryos, the presumptive zygotes were divided and transferred to CR1aa medium supplemented with different concentrations (0 μg/ml, 5 μg/ml, 10 μg/ml and 20 μg/ml) of laminin. To explore the association with sperm-oocyte fusion, oocytes and/or sperm were pre-incubated with laminin or anti-β1 antibody before insemination. Laminin was absent in mature oocytes and could be detected first at the 8-cell stage and then displayed an increasing tendency. Adding 10 μg/ml laminin to the culture medium improved embryonic development including cleavage rate, blastocyst rate, total cell numbers in the blastocyst and cell numbers in the inner cell mass. Laminin inhibited sperm-oocyte fusion when incubated with oocytes and/or sperm before in vitro fertilization, and only integrin-β1 of sperm was involved in sperm-oocyte binding. Inhibition may be caused by blocking β1, but why laminin inhibits fertilization is still unknown. The results suggest that laminin plays an important role during embryonic formation and has a negative function in sperm-oocyte fusion, but improves embryonic development. However, only integrin-β1 is involved in sperm-oocyte binding.

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Effect of different culture systems on adipocyte differentiation-related protein (ADRP) in bovine embryos

Cited by 9 publications

References 54 publications

The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation

The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation

Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation

Laminin‐111 Inhibits Bovine Fertilization but Improves Embryonic Development in vitro, and Receptor Integrin‐β1 is Involved in Sperm–Oocyte Binding

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