Effect of diets differing in protein concentration (low vs medium) and nitrogen source (urea vs soybean meal) on in vitro rumen fermentation and on performance of finishing Italian Simmental bulls

Spanghero, M.; Mason, Federico; Zanfi, C.; Никулина, А А

doi:10.1016/j.livsci.2016.12.004

Cited by 17 publications

(17 citation statements)

References 26 publications

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“…Diets differed in terms of CP concentration (125 and 110 g/kg DM) and N source (SBM or SBM partly replaced by urea, Table 1). Results from the parent study (Spanghero et al 2017) showed no difference between experimental groups regarding DM intakes (range 9.28-9.32 kg/d), while growth rates tended to be more favourable for diets M than for L (1.37 vs. 1.28 kg/d, p < 0.10).…”

Section: Resultsmentioning

confidence: 84%

“…Regarding the down regulation of the AQP3 channel in +U diets it must be considered that a high rumen ammonia level is negatively correlated with urea influx in the rumen (Kennedy and Milligan 1980;Doranalli et al 2011). The diets containing urea had a significant post feeding increment of rumen ammonia concentration in vitro (Spanghero et al 2017) and this probably attenuated the rumen influx of urea by inactivation of the AQP3 channel. Finally, in our study, the mRNA expression of AQP7 and 10 has not shown differences between diets, but we have highlighted a high positive correlation (R 2 = 0.871) between the expressions of these two channels.…”

Section: Discussionmentioning

confidence: 99%

“…All experimental procedures met the requirements of the European Community Directive 2010/63/EU on the protection of animals used for scientific purposes and a detailed description of the in vivo trial can be found in Spanghero et al (2017). In brief, 80 IS young bulls were assigned to 16 pens (5 bulls/pen) and entered in the trial when achieving a body weight (BW) of 495 ± 58 kg (around 14 months of age).…”

Section: Animals and Treatmentsmentioning

confidence: 99%

“…Therefore, the aim of this paper was to study the impact of the dietary crude protein (CP) level (medium vs. low) and source of N (soybean meal vs. urea) on mRNA expression of UT-B urea transporter and some AQP in rumen epithelium of fattening bulls of the breed Italian Simmental (IS). The paper presents analytical results of biological samples (blood and rumen wall) collected at slaughtering from fattening IS bulls used in a feeding trial described by Spanghero et al (2017).…”

Section: Introductionmentioning

confidence: 99%

“…Ingredients, chemical composition and contents of intestinal digestible protein (PDI) of diets differing in crude protein (CP) level (medium[M] or low[L]) and nitrogen source (soybean meal or urea (adapted fromSpanghero et al 2017).…”

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confidence: 99%

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Effect of dietary nitrogen level and source on mRNA expression of urea transporters in the rumen epithelium of fattening bulls

Saccà

Corazzin

Giannico

et al. 2018

Archives of Animal Nutrition

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This paper aims to study the effect of the dietary treatments on mRNA expression of urea transporter B (UT-B) and some aquaporins (AQP) in rumen epithelium of Italian Simmental young bulls. Eighty animals allocated to 16 pens were fed from about 500 to 650 kg body weight with four experimental diets, which resulted from the combination of two crude protein levels (125 and 110 g/kg dry matter, diets M and L, respectively) and two nitrogen sources (soybean meal (SBM) or SBM partly replaced by an isonitrogenous mixture of corn and urea; diets -U and +U, respectively). At slaughtering samples of blood and rumen epithelium were collected from six bulls for each diet. Blood samples were analysed for haematological parameters and quantitative PCR was carried out on the mRNA extracted from the rumen epithelium samples. The bulls fed diets M had lower plasma concentrations of aspartate aminotransferase than those receiving diets L (78.9 vs. 88.3 U/l, p = 0.04). Plasma urea was higher (p = 0.03) for diets M and lower for diets +U (2.0 vs. 2.5 and 1.73 vs. 2.00 mmol/l, respectively, in M and L diets, p = 0.04). The effect of dietary treatments on rumen UT expression were limited to AQP3, which was down regulated (p = 0.01) in diets +U. Finally, a high positive correlation (R = 0.871) between the expressions of AQP7 and AQP10 was found. In conclusion, the AQP3 appears very responsive to dietary treatments and therefore it is a candidate to be further studied in rumen metabolism experiments. The close relationship between mRNA expression of AQP7 and AQP10 indicates a similar function of these two proteins.

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Section: Resultsmentioning

confidence: 84%