Effect of Dietary β-Mannanase Supplementation and Palm Kernel Meal Inclusion on Laying Performance and Egg Quality in 73 Weeks Old Hens

Abstract

This study was conducted to evaluate the effect of dietary β-mannanase supplementation and palm kernel meal (PKM) inclusion (5%) on laying performance, egg quality and nutrient utilizability of laying hens with 73 weeks of age. A total of 240 Lohmann brown laying hens with average 77.5% egg production were randomly allocated with 60 hens per treatment, 4 replicates per treatment and 15 hens per replicate. Experimental design was a completely randomized design with 2 × 2 factorial arrangement, with the factors being (1) two levels of PKM (0 vs. 5%) and (2) with or without dietary β-mannanase (480 IU/kg of diet CTCzyme ® ) supplementation. All hens were housed in cages (35 cmW × 35 cmD × 40 cmH) with 2 hens per cage for six weeks feeding trial. Laying performance was recorded daily during feeding trial. Egg quality, nutrients utilizability and blood assays were done at the end of feeding trial. Egg production was improved (P<0.05) by both dietary PKM inclusion and β-mannanase combined supplementation. Either β-mannanase or PKM did not affect feed intakes and feed conversion ratio of all diets. Egg weight of hens fed diet containing 5% of PKM had heavier (P<0.05) eggs compared with hens fed without PKM. Albumen height was improved (P<0.05) by dietary mannanase supplementation. Crude fat utilization of 5% PKM diet was higher than that of no PKM diet regardless of β-mannanase supplementation. Both DM and total carbohydrate utilization were decreased (P<0.05) in hens fed 5% PKM diet. Serum IgG and yolk IgY contents of PKM groups were lower (P<0.05) than those of no PKM groups. This result showed that 5% PKM diet, independent of dietary β-mannanase supplementation, was able to improve egg production. In addition, dietary β-mannanase supplementation could be used for improving the albumen height of eggs.

<i>β</i>-Mannanase Derived from <i>Bacillus Subtilis WL-7 </i>Improves the Performance of Commercial Laying Hens Fed Low or High Mannan-Based Diets

“…In the present study, we initially analyzed the mannan contents of all feed ingredients and diets with low or high dietary mannan level were formulated to investigate the effects of the mannan level of diets and β -mannanase supplementation on egg production performance, nutrient retention, and blood metabolites in laying hens. The improved egg production performance of laying hens fed diets supplemented with β -mannanase was observed in the present experiment, which is in agreement with the data reported by Lee et al (2013) , who observed the improved egg production performance of laying hens fed maize-soybean or palm kernel meal-based diets that were supplemented with β -mannanase. Similarly, it was reported that dietary supplementation with β -mannanase improved egg production and growth performance in layers and broilers ( Wu et al , 2005 ; Zangiabadi and Torki, 2010 ).…”

“… Ray et al (1982) and Daskiran et al (2004) reported that mannan significantly reduced growth and increased feed conversion ratio in broiler chickens. Previous studies have reported that supplementation of laying hen diets with β -mannanase could improve the egg production performance and nutrient utilization of laying hens ( Wu et al , 2005 ; Zangiabadi and Torki, 2010 ; Lee et al , 2013 ). Among the different types of β -mannanase available, bacterial β -mannanase appears to be the best option due to its high production rate, ease of control, and overall lower cost.…”

Administration of Palm Kernel Meal and ß-Mannanase on Laying Hens Productivity

“…Menurut penelitian Adrizal et al (2011), pemberian bungkil inti sawit sampai 30% dengan enzim pendegradasi serat pada ayam petelur umur 48 minggu tidak memperburuk produksi telur sedangkan, penambahan ß-Mannanase pada bungkil inti sawit 5% dapat meningkatkan produksi telur (Lee et al, 2013). Beberapa peneliti telah menemukan dampak positif degradasi enzimatik ß-Mannanase pada unggas.…”

“…Berat telur dan HDP yang tidak signifikan dapat disebabkan karena jumlah kebutuhan nutrisi yang sudah mencukupi (Kim et al, 2017) dan tidak berkurang sekalipun ada penambaahan bungkil inti sawit hinggga 14% yang mengandung mannan. Parameter HDP dan berat telur senantiasa berbanding lurus dengan FI sebagaimana penelitian penggunaan bungkil inti sawit dan ß-Mannanase pada kualitas telur ayam petelur (Lee et al, 2013).…”

Influence of Hemicell® Addition on Diets Containing Different Levels of Crude Fiber on Performance of Laying Hens

“…Such poor nutrient utilization of PKM diets could be attributed to nonstarch polysaccharides (NSP) which are poorly utilized in poultry digestive system (Meng et al, 2005) and even act as anti-nutritive factors (Marquardt, 1997). Apparently supplementation of Hemicell ® in diet can break down β-mannan to mannooligosaccharides and simple sugar, and thus supply more nutrients for laying hens (Ferket et al, 2002;Hooge, 2004;Lee et al, 2013). Further, such supplementation increased feed intake and egg production in hens aged over 73 weeks.…”